"
Team:DTU-Denmark/Notebook/30 June 2013
From 2013.igem.org
(Difference between revisions)
(Created page with "=208 lab= == Main purposes today == New PCR with x7-polymerase. ==who were in the lab== Kristian ==Procedure== Made PCRs on pZA21, GFP SF TAT, GFP SF Sec and RFP all sampl...") |
(→208 lab) |
||
| Line 22: | Line 22: | ||
==Results== | ==Results== | ||
| - | + | ||
| + | [[File:30.06.13 all PCR products with x7 first part.jpg|thumb|left|The gel from the days PCR. From first lane is a 100 bp ladder and thereafter the well number follows the numbers of the tubes -1. Note that tube number 15 is missing]] | ||
| + | [[File:30.06.13 all PCR products with x7 second part.jpg|thumb|left|The gel from the days PCR. From first lane is a 100 bp ladder and thereafter tube 20,21,22,23 and 24. Note that there is also primer blur in subsequent wells to the right; these are just double test wells to test previous PCR products]] | ||
| + | |||
== Conclusion from today == | == Conclusion from today == | ||
... | ... | ||
Revision as of 03:58, 1 July 2013
Contents |
208 lab
Main purposes today
New PCR with x7-polymerase.
who were in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, GFP SF Sec and RFP all samples where made in duplicates.
In tube 1+2+3+4+5+6 where pZA21. In 7+8+9+10+11+12 GFP SF TAT In 13+14+15+16+17+18 GFP SF Sec In 19+20+21+22+23+24 RFP
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.
Results
Conclusion from today
...
