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if(id == 'd2013630')

{

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ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1>Name of participants: Magnus B, Alexander W, Nils A, Mikael S, Anders E <h2>Ongoing constructs:</h2>75. pEL3A15 - CrtB 25. pSB1C3-CrtY 27. pEL3K16-red 51.pEL3C18-red 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 L. reuteri: Lb1. CF48-pLR581 Lb2. 1063-pLUL631 Lb3. DSM 20016-pVs2 Lb4. 100-23-noplasmid Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP Lb8. 100-23-pGT232 Lb12. DSM 20016-noplasmid Lb14. DSM 20016-pLUL631 <~~strong~~>L. plantarum:</~~strong~~> Lb5. 256-rifR-pAMβ1 L. lactis: Lb13. MG1363-pJP059 Lb15. unknown-pGus Lb16. MG1363-noplasmid Lb10. 256-noplasmid Lb11. 36E-noplasmid Lb9. JH2-2 pAMβ1 <h2>Todays work:</h2> Transformation61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red 75. pEL3A15 - CrtB Digest27. pEL3K16-red 51.pEL3C18-red Ligation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly. O/N Screening25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h3>Results</h3> Re-streak Lb13. Lc. lactis MG1363 pJP059: Good growth Lb16. Lc. lactis MG1363 noplasmid: Good growth Lb15. Lc. lactis MG1363-pGUS: Good growth 19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed 75. pEL3A15 - CrtB failed </div>';

+

ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1>Name of participants: Magnus B, Alexander W, Nils A, Mikael S, Anders E <h2>Ongoing constructs:</h2>E. coli: 75. pEL3A15 - CrtB 25. pSB1C3-CrtY 27. pEL3K16-red 51.pEL3C18-red 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 L. reuteri: Lb1. CF48-pLR581 Lb2. 1063-pLUL631 Lb3. DSM 20016-pVs2 Lb4. 100-23-noplasmid Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP Lb8. 100-23-pGT232 Lb12. DSM 20016-noplasmid Lb14. DSM 20016-pLUL631 L. plantarum: Lb5. 256-rifR-pAMβ1 L. lactis: Lb13. MG1363-pJP059 Lb15. unknown-pGus Lb16. MG1363-noplasmid Lb10. 256-noplasmid Lb11. 36E-noplasmid Lb9. JH2-2 pAMβ1 <h2>Todays work:</h2> Transformation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red 75. pEL3A15 - CrtB Digest 27. pEL3K16-red 51.pEL3C18-red Ligation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly. O/N Screening 25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h3>Results</h3> Re-streak Lb13. Lc. lactis MG1363 pJP059: Good growth Lb16. Lc. lactis MG1363 noplasmid: Good growth Lb15. Lc. lactis MG1363-pGUS: Good growth 19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed 75. pEL3A15 - CrtB failed </div>';

}

else if(id == 'd2013629')

{

-

ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br~~><p~~>Name of participants: Magnus B, Alexander W, Nils~~'+ '~~A, Anders E, Mikael S '+

+

ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1> Name of participants: Magnus B, Alexander W, Nils A, Anders E, Mikael S <h2>Ongoing constructs:</h2>L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli (strain D5α): 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24.B0034-CrtI 38. pEL3A16-red 42. pSB4S15-red 57.B0034-Idi 75. pEL3A15-CrtB 76. pEL3K16-CrtI 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Todays work</h2>PCR purification: CP1 CP8 CP11 CP29 CP30 CP41 Cp44 Digestion: 27. pEL3K16-red 51.pEL3C18-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR) Ligation: 24.3 B0034-CrtI -> 27. pEL3K16-red 23.2 pSB1C3-CrtB -> 38. pEL3A16-red Transformation: 75. pEL3A15 – CrtB* 76. pEL3K16 – CrtI* *Cancelled, wrong solution was used -> redo tomorrow. Re-streak: 1.2 pSB1C3-red (from frozen stock) 7. pSB3K3 (”) 60. PK401 dam Plasmid preparation: 42. pSB4S15-red O/N Screening PCR: 25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF (19,20,21(with DMSO dilution series(7,9,12)) PCR (screening?): 57.B0034-Idi IspA Gel electrophoresis: 57.B0034-Idi IspA 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 38. pEL3A16-red (digest) 23.1 pSB1C3-CrtB (digest) 23.2 pSB1C3-CrtB (digest) 27. pEL3K16-red (digest) 24.3 B0034-CrtI (digest) 24.4 B0034-CrtI (digest) <h2>Results</h2>Today: Plasmid preparation: 42.1 pSB4S15-red klon 1: 27.4 ng/µL 42.2 pSB4S15-red klon 2: 21.8 ng/µL 42.3 pSB4S15-red klon 3: 24.3 ng/µL 42.4 pSB4S15-red klon 4: 25.3 ng/µL Gel electrophoresis: 57.B0034-Idi: still not good after three attempts IspA: eventually good 38. pEL3A16-red (digest): failed, redo subcloning tomorrow 23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 27. pEL3K16-red (digest): succeded 24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow 24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow Transformation: 75. pEL3A15 - CrtB failed PCR purification (of promotors):* CP1: 40 ng/µl CP8: 41,3 ng/µl CP11: 46,7 ng/µl CP29: 40 ng/µl CP30: 58 ng/µl CP41: 41,9 ng/µl Cp44: 42,3 ng/µl *PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor constructs (not 77) from annealing of R and F (without PCR-cleaning). Previous day: ON screening: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions </div>';

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'<h2>Ongoing constructs:</h2>'+

+

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'L. reuteri: '+

+

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'1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP '+

+

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'8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 '+

+

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'10. 256-noplasmid 11. 36E-noplasmid '+

+

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'E. faecalis: '+

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'9. JH2-2 pAMβ1 '+

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'L. lactis: '+

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'13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid '+

+

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'E.coli (strain D5α): '+

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'19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

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'23. pSB1C3-CrtB 24.B0034-CrtI 38. pEL3A16-red 42. pSB4S15-red 57.B0034-Idi 75. pEL3A15-CrtB '+

+

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'76. pEL3K16-CrtI 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

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'66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP '+

+

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'71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 '+

+

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'<h2>Todays work</h2>'+

+

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'PCR purification: '+

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'CP1 CP8 CP11 CP29 CP30 CP41 Cp44 '+

+

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'Digestion: '+

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'27. pEL3K16-red 51.pEL3C18-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

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'66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP '+

+

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'71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP '+

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'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR) '+

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'Ligation: '+

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'24.3 B0034-CrtI -> 27. pEL3K16-red 23.2 pSB1C3-CrtB -> 38. pEL3A16-red '+

+

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'Transformation: '+

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'75. pEL3A15 – CrtB* 76. pEL3K16 – CrtI* *Cancelled, wrong solution was used -> redo tomorrow. '+

+

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'Re-streak: '+

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'1.2 pSB1C3-red (from frozen stock) 7. pSB3K3 (”) 60. PK401 dam '+

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'Plasmid preparation: '+

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'42. pSB4S15-red '+

+

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'O/N Screening PCR: '+

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'25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

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'(19,20,21(with DMSO dilution series(7,9,12)) '+

+

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'PCR (screening?): '+

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'57.B0034-Idi IspA '+

+

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'Gel electrophoresis: '+

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'57.B0034-Idi IspA 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF '+

+

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'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 38. pEL3A16-red (digest) 23.1 pSB1C3-CrtB (digest) 23.2 pSB1C3-CrtB (digest) '+

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'27. pEL3K16-red (digest) 24.3 B0034-CrtI (digest) 24.4 B0034-CrtI (digest) '+

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'<h2>Results</h2>'+

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'Today: '+

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'Plasmid preparation: '+

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'42.1 pSB4S15-red klon 1: 27.4 ng/µL 42.2 pSB4S15-red klon 2: 21.8 ng/µL 42.3 pSB4S15-red klon 3: 24.3 ng/µL '+

+

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'42.4 pSB4S15-red klon 4: 25.3 ng/µL '+

+

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'Gel electrophoresis: '+

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'57.B0034-Idi: still not good after three attempts IspA: eventually good 38. pEL3A16-red (digest): failed, redo subcloning tomorrow '+

+

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'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow '+

+

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'27. pEL3K16-red (digest): succeded 24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow '+

+

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'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow '+

+

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'Transformation: '+

+

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'75. pEL3A15 - CrtB failed '+

+

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'PCR purification (of promotors):* '+

+

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'CP1: 40 ng/µl CP8: 41,3 ng/µl CP11: 46,7 ng/µl CP29: 40 ng/µl CP30: 58 ng/µl CP41: 41,9 ng/µl Cp44: 42,3 ng/µl '+

+

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'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+

+

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'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+

+

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'constructs (not 77) from annealing of R and F (without PCR-cleaning). '+

+

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'Previous day: '+

+

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'ON screening: '+

+

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'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions '+

+

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'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions <br~~></p~~></div>';

+

}

else if(id == 'd2013628')

{

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ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1~~><p~~>Name of participants: '+

+

ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1>Name of participants: Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W <h2>Ongoing constructs:</h2>L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57.B0034-Idi ispA <h2>Todays work</h2>Frozen stock: Lb13. Lc. lactis MG1363 pJP059 clone 2 Lb15. Lc. lactis unknown pGus clone 2 Lb16. Lc. lactis MG1363 noplasmid clone 2 Digest: 25.pSB1C3-CrtY Transformation: 24.pSB1C3-CrtI 23.pSB1C3-CrtB Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)” Lb15. Lc. lactis MG1363 pGus clone 2 “I, II, III(2x1.5 ml culture)” 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 Re-streaks: Lb3. reuteri DSM20016 pVS2 Lb8. reuteri 100-23 pGT232* Lb11. plantarum 36E Lb13. Lc. lactis MG1363 pJP059 Lb16. Lc. lactis MG1363 noplasmid *from frozen stock Streaking of strains: Lb15. Lc. lactis MG1363 plasmid unknown pGus: Lb18. Lb. delbrueckii bulgaricus (from freeze dried yoghurt culture) Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) O/N of restreak from Erik Gullberg: 42. pSB4S15-red, clones 1 and 2 PCR: 57.B0034-Idi* ispA* 19.1** 20.1** 21.1** * Amplified from D5α-chromosome. ** Now with DMSO Gel electrophoresis: 57.B0034-Idi ispA 19.1 20.1 21.1 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS O/N Screening: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 <h2>Results</h2>Previous day: Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4) → Store in freezer, try again with new protocol Transformation: Lb10. plantarum 256 pLUL364: No growth Lb11. plantarum 36E pVS2: No growth Lb12. Lc. lactis DSM20016-pVS2: No growth → Try again later with new competent cells Wednesday 26/6: Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with antibiotics Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today Today: Plasmid preparation: Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “II”: 7,5 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl → Mark and store in freezer. Gel electrophoresis: 57.B0034-Idi: hard do decipher results, redo tomorrow ispA: (“) 19.1: failed 20.1: failed 21.1: failed 43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28) 44. pSB1C3-B0034-His-TAL: failed (“) 45. pSB1C3-B0034-4CL: failed (“) 46. pSB1C3-B0034-His-4CL: failed (“) 47. pSB1C3-B0034-STS: failed (image 2 2013.06.28) 48. pSB1C3-B0034-His-STS: failed (“) <h2>Other experiments</h2>THMS buffer preparation Aliquoting of sigma water Preparation of antibiotic-free LB-agar plates Dilution of promotor-oligonucleotides from stock to 5 µM: CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R CP29_F CP29_R CP41_F CP41_R Dilution of promotor oligonucleotide from pellet to 100 µM: usp45_mut_F Phosphorylation of promotor-oligonucleotides: CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R CP29_F CP29_R CP41_F CP41_R</div>';

-

'Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M.,'+

+

-

' Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W '+

+

-

'<h2>Ongoing constructs:</h2>'+

+

-

'L. reuteri: '+

+

-

'1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid '+

+

-

'6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid '+

+

-

'14. DSM 20016-pLUL631(B?) '+

+

-

'L. plantarum: '+

+

-

'5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 '+

+

-

'L. lactis: '+

+

-

'13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid '+

+

-

'E.coli: '+

+

-

'3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 15. pSB4K5-RBS-TAL'+

+

-

'-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034'+

+

-

'-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034'+

+

-

'-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034'+

+

-

'-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034'+

+

-

'-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3'+

+

-

'-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3'+

+

-

'-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. '+

+

-

'pSB1C3-B0034-His-STS 57.B0034-Idi ispA '+

+

-

'<h2>Todays work</h2>'+

+

-

'Frozen stock: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059 clone 2 Lb15. Lc. lactis unknown pGus clone 2 Lb16. Lc. lactis'+

+

-

' MG1363 noplasmid clone 2 '+

+

-

'Digest: '+

+

-

'25.pSB1C3-CrtY '+

+

-

'Transformation: '+

+

-

'24.pSB1C3-CrtI 23.pSB1C3-CrtB '+

+

-

'Plasmid preparation: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)” Lb15. Lc. lactis MG1363 '+

+

-

'pGus clone 2 “I, II, III(2x1.5 ml culture)” 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 '+

+

-

'7. pSB3K3-red 11. pSB4A15 '+

+

-

'Re-streaks: '+

+

-

'Lb3. reuteri DSM20016 pVS2 Lb8. reuteri 100-23 pGT232* Lb11. plantarum 36E Lb13. Lc. lactis'+

+

-

' MG1363 pJP059 Lb16. Lc. lactis MG1363 noplasmid *from frozen stock '+

+

-

'Streaking of strains: '+

+

-

'Lb15. Lc. lactis MG1363 plasmid unknown pGus: Lb18. Lb. delbrueckii bulgaricus (from freeze dried'+

+

-

' yoghurt culture) Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) '+

+

-

'O/N of restreak from Erik Gullberg: '+

+

-

'42. pSB4S15-red, clones 1 and 2 '+

+

-

'PCR: '+

+

-

'57.B0034-Idi* ispA* 19.1** 20.1** 21.1** * Amplified from D5α-chromosome. '+

+

-

'** Now with DMSO '+

+

-

'Gel electrophoresis: '+

+

-

'57.B0034-Idi ispA 19.1 20.1 21.1 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL '+

+

-

'45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-'+

+

-

'STS '+

+

-

'O/N Screening: '+

+

-

'19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker'+

+

-

'-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC'+

+

-

'-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker'+

+

-

'-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-'+

+

-

'CrtB-B0034-CrtI-Linker-Zif268 '+

+

-

'<h2>Results</h2>'+

+

-

'Previous day: '+

+

-

'Plasmid preparation: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4) → Store in freezer, try again '+

+

-

'with new protocol '+

+

-

'Transformation: '+

+

-

'Lb10. plantarum 256 pLUL364: No growth Lb11. plantarum 36E pVS2: No growth Lb12. Lc. lactis '+

+

-

'DSM20016-pVS2: No growth → Try again later with new competent cells '+

+

-

'Wednesday 26/6: '+

+

-

'Streaking of strains: '+

+

-

'Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today '+

+

-

'Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with '+

+

-

'antibiotics Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations '+

+

-

'(1x, 5x & 10x) → Re-streak today '+

+

-

'Today: '+

+

-

'Plasmid preparation: '+

+

-

'Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 '+

+

-

'“II”: 7,5 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl '+

+

-

'Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl'+

+

-

' Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl → Mark and store in freezer. '+

+

-

'Gel electrophoresis: '+

+

-

'57.B0034-Idi: hard do decipher results, redo tomorrow ispA: (“) 19.1: failed 20.1: failed'+

+

-

' 21.1: failed 43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28) 44. pSB1C3-B0034-His-TAL: '+

+

-

'failed (“) 45. pSB1C3-B0034-4CL: failed (“) 46. pSB1C3-B0034-His-4CL: failed (“) 47. '+

+

-

'pSB1C3-B0034-STS: failed (image 2 2013.06.28) 48. pSB1C3-B0034-His-STS: failed (“) '+

+

-

'<h2>Other experiments</h2>'+

+

-

'THMS buffer preparation '+

+

-

'Aliquoting of sigma water '+

+

-

'Preparation of antibiotic-free LB-agar plates '+

+

-

'Dilution of promotor-oligonucleotides from stock to 5 µM: '+

+

-

'CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R '+

+

-

'CP29_F CP29_R CP41_F CP41_R '+

+

-

'Dilution of promotor oligonucleotide from pellet to 100 µM: '+

+

-

'usp45_mut_F '+

+

-

'Phosphorylation of promotor-oligonucleotides: '+

+

-

'CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R '+

+

-

'CP29_F CP29_R CP41_F CP41_R~~ ~~</div>';

+

}

else if(id == 'd2013627')

@@ Line 211: / Line 211: @@
   if(id == 'd2013630')
   {
-  ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Mikael S, Anders E<br><br><h2>Ongoing constructs:</h2>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><b>L. reuteri:</b><br>Lb1. CF48-pLR581<br>Lb2. 1063-pLUL631<br>Lb3. DSM 20016-pVs2<br>Lb4. 100-23-noplasmid<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br>Lb8. 100-23-pGT232<br>Lb12. DSM 20016-noplasmid<br>Lb14. DSM 20016-pLUL631<br><br><strong>L. plantarum:</strong><br>Lb5. 256-rifR-pAMβ1<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br>Lb16. MG1363-noplasmid<br><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br>Lb9. JH2-2 pAMβ1<br><br><h2>Todays work:</h2><br><b>Transformation</b>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>75. pEL3A15 - CrtB<br><br><b>Digest</b>27. pEL3K16-red<br>51.pEL3C18-red<br><br><b>Ligation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly.<br><br><b>O/N Screening</b>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h3>Results</h3><br><b>Re-streak</b><br>Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br></div>';
+  ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Mikael S, Anders E<br><br><h2>Ongoing constructs:</h2><b>E. coli:</b><br>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><b>L. reuteri:</b><br>Lb1. CF48-pLR581<br>Lb2. 1063-pLUL631<br>Lb3. DSM 20016-pVs2<br>Lb4. 100-23-noplasmid<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br>Lb8. 100-23-pGT232<br>Lb12. DSM 20016-noplasmid<br>Lb14. DSM 20016-pLUL631<br><br><b>L. plantarum:</b><br>Lb5. 256-rifR-pAMβ1<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br>Lb16. MG1363-noplasmid<br><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br>Lb9. JH2-2 pAMβ1<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>75. pEL3A15 - CrtB<br><br><b>Digest</b><br>27. pEL3K16-red<br>51.pEL3C18-red<br><br><b>Ligation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly.<br><br><b>O/N Screening</b><br>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h3>Results</h3><br><b>Re-streak</b><br>Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br></div>';
   }
   else if(id == 'd2013629')
   {
- ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><p><b>Name of participants:</b> Magnus B, Alexander W, Nils'+ 'A, Anders E, Mikael S<br><br>'+
+	ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Anders E, Mikael S<br><br><h2>Ongoing constructs:</h2><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli (strain D5α):</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Todays work</h2><b>PCR purification:</b><br>CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br><b>Digestion:</b><br>27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br><b>Ligation:</b><br>24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br><b>Transformation:</b><br>75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br><b>Re-streak:</b><br>1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br><b>Plasmid preparation:</b><br>42. pSB4S15-red<br><br><b>O/N Screening PCR:</b><br>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>(19,20,21(with DMSO dilution series(7,9,12))<br><br><b>PCR (screening?):</b><br>57.B0034-Idi<br>IspA<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br><h2>Results</h2><b>Today:</b><br><b>Plasmid preparation:</b><br>42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br><b>Transformation:</b><br>75. pEL3A15 - CrtB failed<br><br><b>PCR purification (of promotors):*</b><br>CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor constructs (not 77) from annealing of R and F (without PCR-cleaning).<br><br><b>Previous day:</b><br><b>ON screening:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></div>';
-'<h2>Ongoing constructs:</h2>'+
-'<b>L. reuteri:</b><br>'+
-'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>'+
-'8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>'+
-'10. 256-noplasmid<br>11. 36E-noplasmid<br><br>'+
-'<b>E. faecalis:</b><br>'+
-'9. JH2-2 pAMβ1<br><br>'+
-'<b>L. lactis:</b><br>'+
-'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
-'<b>E.coli (strain D5α):</b><br>'+
-'19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
-'23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>'+
-'76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
-'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
-'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+
-'<h2>Todays work</h2>'+
-'<b>PCR purification:</b><br>'+
-'CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br>'+
-'<b>Digestion:</b><br>'+
-'27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
-'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
-'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>'+
-'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br>'+
-'<b>Ligation:</b><br>'+
-'24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br>'+
-'<b>Transformation:</b><br>'+
-'75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br>'+
-'<b>Re-streak:</b><br>'+
-'1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'42. pSB4S15-red<br><br>'+
-'<b>O/N Screening PCR:</b><br>'+
-'25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
-'(19,20,21(with DMSO dilution series(7,9,12))<br><br>'+
-'<b>PCR (screening?):</b><br>'+
-'57.B0034-Idi<br>IspA<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
-'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>'+
-'27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br>'+
-'<h2>Results</h2>'+
-'<b>Today:</b><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>'+
-'42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>'+
-'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>'+
-'27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>'+
-'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br>'+
-'<b>Transformation:</b><br>'+
-'75. pEL3A15 - CrtB failed<br><br>'+
-'<b>PCR purification (of promotors):*</b><br>'+
-'CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>'+
-'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+
-'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+
-'constructs (not 77) from annealing of R and F (without PCR-cleaning).<br><br>'+
-'<b>Previous day:</b><br>'+
-'<b>ON screening:</b><br>'+
-'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
-'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
   }
 else if(id == 'd2013628')
   {
-  ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1><p><b>Name of participants: </b>'+
+	ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1><b>Name of participants: </b>Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W<br><br><h2>Ongoing constructs:</h2><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>ispA<br><br><h2>Todays work</h2><b>Frozen stock:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 2<br>Lb15. Lc. lactis unknown pGus clone 2<br>Lb16. Lc. lactis MG1363 noplasmid clone 2<br><br><b>Digest:</b><br>25.pSB1C3-CrtY<br><br><b>Transformation:</b><br>24.pSB1C3-CrtI<br>23.pSB1C3-CrtB<br><br><b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)”<br>Lb15. Lc. lactis MG1363 pGus clone 2 “I, II, III(2x1.5 ml culture)”<br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br><br><b>Re-streaks:</b><br> Lb3. reuteri DSM20016 pVS2<br>Lb8. reuteri 100-23 pGT232*<br>Lb11. plantarum 36E<br>Lb13. Lc. lactis MG1363 pJP059<br>Lb16. Lc. lactis MG1363 noplasmid<br>*from frozen stock<br><br><b>Streaking of strains:</b><br> Lb15. Lc. lactis MG1363 plasmid unknown pGus:<br>Lb18. Lb. delbrueckii bulgaricus (from freeze dried yoghurt culture) <br>Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) <br><br><b>O/N of restreak from Erik Gullberg:</b><br>42. pSB4S15-red, clones 1 and 2<br><br><b>PCR:</b><br>57.B0034-Idi*<br>ispA*<br>19.1**<br>20.1**<br>21.1**<br>* Amplified from D5α-chromosome. <br>** Now with DMSO<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi<br>ispA<br>19.1<br>20.1<br>21.1<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>O/N Screening:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br><br><h2>Results</h2><b>Previous day:</b><br><b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4)<br>→ Store in freezer, try again with new protocol <br><br><b>Transformation:</b><br> Lb10. plantarum 256 pLUL364: No growth<br>Lb11. plantarum 36E pVS2: No growth<br>Lb12. Lc. lactis DSM20016-pVS2: No growth<br>→ Try again later with new competent cells<br><br><b>Wednesday 26/6:</b><br><b>Streaking of strains:</b><br> Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br>Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with antibiotics<br>Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br><br><b>Today:</b><br> <b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “II”: 7,5 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl <br>Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ<br>Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl<br>Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl<br>→ Mark and store in freezer.<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi: hard do decipher results, redo tomorrow <br>ispA: (“)<br>19.1: failed<br>20.1: failed<br>21.1: failed<br>43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>46. pSB1C3-B0034-His-4CL: failed (“)<br>47. pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br><br><h2>Other experiments</h2><b>THMS buffer preparation</b><br><b>Aliquoting of sigma water</b><br><b>Preparation of antibiotic-free LB-agar plates</b><br><b>Dilution of promotor-oligonucleotides from stock to 5 µM:</b><br>CP1_F<br>CP1_R<br>CP11_F<br>CP11_R <br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F <br>CP30_R <br>CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br><b>Dilution of promotor oligonucleotide from pellet to 100 µM:</b><br>usp45_mut_F<br><br><b>Phosphorylation of promotor-oligonucleotides:</b><br>CP1_F <br>CP1_R <br>CP11_F <br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>CP29_F<br>CP29_R<br>CP41_F<br>CP41_R</div>';
-'Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M.,'+
-' Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W<br><br>'+
-'<h2>Ongoing constructs:</h2>'+
-'<b>L. reuteri:</b><br>'+
-'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>'+
-'6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>'+
-'14. DSM 20016-pLUL631(B?)<br><br>'+
-'<b>L. plantarum:</b><br>'+
-'5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br>'+
-'<b>L. lactis:</b><br>'+
-'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
-'<b>E.coli:</b><br>'+
-'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL'+
-'-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034'+
-'-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034'+
-'-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034'+
-'-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034'+
-'-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3'+
-'-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3'+
-'-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. '+
-'pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>ispA<br><br>'+
-'<h2>Todays work</h2>'+
-'<b>Frozen stock:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059 clone 2<br>Lb15. Lc. lactis unknown pGus clone 2<br>Lb16. Lc. lactis'+
-' MG1363 noplasmid clone 2<br><br>'+
-'<b>Digest:</b><br>'+
-'25.pSB1C3-CrtY<br><br>'+
-'<b>Transformation:</b><br>'+
-'24.pSB1C3-CrtI<br>23.pSB1C3-CrtB<br><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)”<br>Lb15. Lc. lactis MG1363 '+
-'pGus clone 2 “I, II, III(2x1.5 ml culture)”<br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>'+
-'7. pSB3K3-red<br>11. pSB4A15<br><br>'+
-'<b>Re-streaks:</b><br>'+
-'Lb3. reuteri DSM20016 pVS2<br>Lb8. reuteri 100-23 pGT232*<br>Lb11. plantarum 36E<br>Lb13. Lc. lactis'+
-' MG1363 pJP059<br>Lb16. Lc. lactis MG1363 noplasmid<br>*from frozen stock<br><br>'+
-'<b>Streaking of strains:</b><br>'+
-'Lb15. Lc. lactis MG1363 plasmid unknown pGus:<br>Lb18. Lb. delbrueckii bulgaricus (from freeze dried'+
-' yoghurt culture) <br>Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) <br><br>'+
-'<b>O/N of restreak from Erik Gullberg:</b><br>'+
-'42. pSB4S15-red, clones 1 and 2<br><br>'+
-'<b>PCR:</b><br>'+
-'57.B0034-Idi*<br>ispA*<br>19.1**<br>20.1**<br>21.1**<br>* Amplified from D5α-chromosome. <br>'+
-'** Now with DMSO<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi<br>ispA<br>19.1<br>20.1<br>21.1<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>'+
-'45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-'+
-'STS<br><br>'+
-'<b>O/N Screening:</b><br>'+
-'19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker'+
-'-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC'+
-'-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker'+
-'-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-'+
-'CrtB-B0034-CrtI-Linker-Zif268<br><br>'+
-'<h2>Results</h2>'+
-'<b>Previous day:</b><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4)<br>→ Store in freezer, try again '+
-'with new protocol <br><br>'+
-'<b>Transformation:</b><br>'+
-'Lb10. plantarum 256 pLUL364: No growth<br>Lb11. plantarum 36E pVS2: No growth<br>Lb12. Lc. lactis '+
-'DSM20016-pVS2: No growth<br>→ Try again later with new competent cells<br><br>'+
-'<b>Wednesday 26/6:</b><br>'+
-'<b>Streaking of strains:</b><br>'+
-'Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br>'+
-'Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with '+
-'antibiotics<br>Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations '+
-'(1x, 5x & 10x) → Re-streak today<br><br>'+
-'<b>Today:</b><br>'+
-'<b>Plasmid preparation:</b><br>'+
-'Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 '+
-'“II”: 7,5 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl <br>'+
-'Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ<br>Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl'+
-'<br>Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl<br>→ Mark and store in freezer.<br><br>'+
-'<b>Gel electrophoresis:</b><br>'+
-'57.B0034-Idi: hard do decipher results, redo tomorrow <br>ispA: (“)<br>19.1: failed<br>20.1: failed'+
-'<br>21.1: failed<br>43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: '+
-'failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>46. pSB1C3-B0034-His-4CL: failed (“)<br>47. '+
-'pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br><br>'+
-'<h2>Other experiments</h2>'+
-'<b>THMS buffer preparation</b><br>'+
-'<b>Aliquoting of sigma water</b><br>'+
-'<b>Preparation of antibiotic-free LB-agar plates</b><br>'+
-'<b>Dilution of promotor-oligonucleotides from stock to 5 µM:</b><br>'+
-'CP1_F<br>CP1_R<br>CP11_F<br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>'+
-'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br>'+
-'<b>Dilution of promotor oligonucleotide from pellet to 100 µM:</b><br>'+
-'usp45_mut_F<br><br>'+
-'<b>Phosphorylation of promotor-oligonucleotides:</b><br>'+
-'CP1_F<br>CP1_R<br>CP11_F<br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>'+
-'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br></p></div>';
   }
 else if(id == 'd2013627')

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