Team:UT Dallas/week8

From 2013.igem.org

(Difference between revisions)

Latest revision as of 02:08, 28 September 2013

<a name="h.mdl7xxxdjt4h"></a>8/23

<a name="h.5xsqt7ge99ee"></a>Miniprep/nanodrop

<a name="h.ctzr6b52iix8"></a>i5-45-2 ComA → i5-46-14 → 23.4 ng/ul

<a name="h.gja1o1xcsept"></a>i5-45-3 ComA → i5-46-15

<a name="h.c02sd7dnsuxi"></a>i5-43-12 (CP- Scr-pSur - RBS-GFP) → i5-46-16 → 250 ng/ul

<a name="h.v826cclo51xq"></a>i5-11-5 →129.8

<a name="h.a133mmz89cfs"></a>i5-11-5 →129.9

<a name="h.m6kr3jcqty82"></a>i5-11-5 →157.6

<a name="h.wpg2no967d3i"></a>i5-11-5 →125.4

<a name="h.ylsjxgz2umn1"></a>i5-11-5 →112.5

<a name="h.w0dwoegdqnoo"></a>Test Digestion

<a name="h.utwwqkctwand"></a>E/P used

<a name="h.4jvmna5jr96h"></a>	<a name="h.w6isy7n1z7ha"></a>i5-46-14	<a name="h.qma88sakg5g"></a>i5-46-15	<a name="h.af2dhot2q19u"></a>i5-46-16
<a name="h.mweie01wos47"></a>DNA	<a name="h.x7agldda9we4"></a>10	<a name="h.45ua2a67k5ak"></a>11	<a name="h.gjmx3d51vw6d"></a>1
<a name="h.20zvhl9aedd3"></a>Buffer	<a name="h.ceih3pa3mra4"></a>2	<a name="h.j00xod57omxh"></a>2	<a name="h.1js16x5pnmwb"></a>2
<a name="h.uusqov85j404"></a>E	<a name="h.9rw52ce0ezcv"></a>0	<a name="h.e02k9fcsbmmu"></a>0	<a name="h.ijynz4yv0qah"></a>1
<a name="h.ux6fp0ay10jy"></a>P	<a name="h.m1cymlp4oruj"></a>1	<a name="h.hlb3ljrx8b46"></a>1	<a name="h.grt85umlt6kt"></a>1
<a name="h.l5srj41q69np"></a>Water	<a name="h.7i29fh4kdg3"></a>7	<a name="h.xjal23kcxves"></a>6	<a name="h.slues9am83vc"></a>15
<a name="h.ai4na2btkv19"></a>Total	<a name="h.ghbiqqy43fx"></a>20	<a name="h.6sdztehr1ah5"></a>20	<a name="h.sin6a82e27eb"></a>20
<a name="h.a3fhmuu8alye"></a>	<a name="h.u12h1jpzx0g2"></a>1	<a name="h.g4y0epj01up4"></a>2	<a name="h.fe0gi7ev1hhd"></a>3

<a name="h.hjb6jkt8i9j6"></a>Picked from glycerol stock

<a name="h.e2mgxko1y8we"></a>i5-47-1 ComB → from i5-22-1

<a name="h.dsgka111eja8"></a>i5-47-2 Com C → from i5-22-4

<a name="h.xrujmyk02uc0"></a>i5-22-5 Scr

<a name="h.bw8baiv3ogae"></a>i5-22-6 pFru

<a name="h.u4i83vrwug3b"></a>8/24

<a name="h.ptn8jdlrr7w3"></a>Miniprep and Nanodrop

<a name="h.tmxjgtc0kp2l"></a>i5-46-10 → 68.9

<a name="h.cnp2rwemrkux"></a>i5-46-11→ 40.4

<a name="h.hgyx1mskxf1w"></a>i5-46-1 → 198.2

<a name="h.2fo4ma7ic9b0"></a>i5-46-2 → 160.1

<a name="h.fonkqhabrm56"></a>ComA mutagenesis #1 (attempt 2)

<a name="h.eajl4vssk8vz"></a>	<a name="h.cq4v0p75fhn4"></a>i5-40-1
<a name="h.eu841w90i5ee"></a>QS	<a name="h.zjvg2gl8bkj"></a>25
<a name="h.jc8jzw6rg3dt"></a>F-primer	<a name="h.quc8laf5n2wn"></a>1.1
<a name="h.t73em28z47jq"></a>R-primer	<a name="h.28dhcswukz7r"></a>1.1
<a name="h.o31epurx3w35"></a>DNA	<a name="h.2k1sml5dv115"></a>1
<a name="h.yq3kvf6ecuau"></a>Water	<a name="h.mq838o83wvu8"></a>22
<a name="h.7lyxy4jpewm3"></a>Total	<a name="h.mzc8i2vwrc2l"></a>50
<a name="h.790r9g43m7b0"></a>	<a name="h.nam1h6z4oqhk"></a>i5-48-1

<a name="h.opisrjjglaut"></a>8/24

<a name="h.9fkso0reaxid"></a>Test Digestion

<a name="h.av8bh9leadg2"></a>	<a name="h.937o07p46yh0"></a>i5-46-10	<a name="h.qoa3kq48ld9s"></a>i5-46-11
<a name="h.lxxzdnyedh0v"></a>DNA	<a name="h.5tiy5wjwas98"></a>4.5	<a name="h.tpd8h8ot67u0"></a>7.5
<a name="h.tof73lpt01uh"></a>Buffer	<a name="h.f3n4bu52grue"></a>2	<a name="h.o3xwyz9lhwsr"></a>2
<a name="h.aze4k6hm3f2c"></a>X	<a name="h.79sa6kc0xyw"></a>2	<a name="h.q0hax18eg4og"></a>2
<a name="h.amtl2yc5gtdk"></a>S	<a name="h.2kv0rbtkabhj"></a>2	<a name="h.bey9n683gs87"></a>2
<a name="h.j7vqnmqzj303"></a>Water	<a name="h.at8k5b7e9nmc"></a>9.5	<a name="h.75ot1094hg26"></a>6.5
<a name="h.626j8f7huchj"></a>Total	<a name="h.opk0dtqcu2er"></a>20	<a name="h.o9cttmwxa8i9"></a>20
<a name="h.apfttnkfbz1x"></a>	<a name="h.6qonn7toamjb"></a>1	<a name="h.gdvxojmpb711"></a>2

<a name="h.5oi3ljbgr3mj"></a>	<a name="h.oaerhkteii3o"></a>-5-46-20	<a name="h.ww2lyic5v41z"></a>15-46-11
<a name="h.o0qt8dt13d4n"></a>DNA	<a name="h.oay07o87p1k9"></a>4.5	<a name="h.t52aw9ml8nn9"></a>7.5
<a name="h.6vyfe3lnvbhb"></a>Buffer	<a name="h.xuifyolcredz"></a>2	<a name="h.vnkvn7dxjueh"></a>2
<a name="h.rhrll65pumag"></a>E	<a name="h.8iumcsv4f9c9"></a>2	<a name="h.l0kksl5wcpgp"></a>2
<a name="h.20im5rrj82mx"></a>Water	<a name="h.kcvh9bujka92"></a>11.5	<a name="h.nfemvspw05pr"></a>8.5
<a name="h.vmj0rt4ol9q6"></a>Total	<a name="h.9ozg7f4aizjs"></a>20	<a name="h.uzy9t4lyti1j"></a>20
<a name="h.8hlsu9obui5o"></a>	<a name="h.1eidt6ph9hhi"></a>3	<a name="h.8isovkrkpecs"></a>4

<a name="h.8bmlbu8dgtkd"></a>Digestion

<a name="h.g6359lfayv0o"></a>	<a name="h.xedyysfsnvi2"></a>i5-47-1 ComB	<a name="h.wmwgz93pllb4"></a>i5-47-2 ComC
<a name="h.y88xro578pw7"></a>DNA	<a name="h.noacn9y9ei3o"></a>10	<a name="h.jzvx5h6yrbku"></a>12.5
<a name="h.v41g7kbsny7f"></a>Buffer	<a name="h.3iotgafuvcim"></a>5	<a name="h.cwh670qlrx6t"></a>5
<a name="h.sy2g5omkqw51"></a>E	<a name="h.27djc45aaoqy"></a>0	<a name="h.xcllte99hz9i"></a>0
<a name="h.45c20xmk97kl"></a>X	<a name="h.a1w7vywdfjw1"></a>1	<a name="h.zed4jb1ehc6d"></a>0
<a name="h.qr9m788dha7q"></a>S	<a name="h.28mooqcl9msj"></a>0	<a name="h.cjvi5jxli9o1"></a>1
<a name="h.kglnxw624vxq"></a>P	<a name="h.g66qdacedbt1"></a>1	<a name="h.7p6us0h2mchy"></a>1
<a name="h.c9ysqtjz68es"></a>Water	<a name="h.sxyrab5wg1w0"></a>33	<a name="h.52asrxxrsaz9"></a>30.5
<a name="h.4hiyrvjph74t"></a>Total	<a name="h.s4od6vlql4jg"></a>50	<a name="h.6ez910a6d9nk"></a>50

<a name="h.q4yq7ejlqme2"></a>Glycerol stock

<a name="h.hk79umej219x"></a>made from i5-46-12, i5-46-13

<a name="h.qucz63u7f4un"></a>Miniprep/nanodrop

<a name="h.jglx03ebwusl"></a>i5-22-5 (ScR) → i5-49-1 → 151.3

<a name="h.4faac6oo6m92"></a>i5-22-6 (pFru) → i5-49-2 → 243.3

<a name="h.wa97pgbvpzfj"></a>i5-46-12 (CP-Cra-pFru-RBS-GFP) → i5-49-3 → 144.5

<a name="h.4dvg4275wfg9"></a>i5-46-13 (CP-ScR-pFru-RBS-GFP) → i5-49-4 → 294.8

<a name="h.yi49iqrxpeyv"></a>Transformation

<a name="h.blp16xoar170"></a>i5-48-1 → i5-49-5 → 20 colonies

<a name="h.8hpm03ekc96b"></a>+ control (i5-11-5)

<a name="h.2tf6ooi2s6dj"></a>i5-46-16 → i5-49-6 → 30 colonies

<a name="h.wmc4rwad9ogm"></a>+ control (i5-11-5) → 150+ colonies

<a name="h.vofk4hpdzlfx"></a>Miniprep

<a name="h.rjlj715ycbc3"></a>i5-49-1 ComB → 150.7

<a name="h.xdprn312zwgg"></a>i5-49-2 ComC → 129.3

<a name="h.xj9616emb6zw"></a>Digestion

<a name="h.crhm9o1ap5ia"></a>	<a name="h.qw10ikui1bss"></a>i5-49-1	<a name="h.sdsmjcv45kyk"></a>i5-49-2
<a name="h.7tnkjt38aq0f"></a>DNA	<a name="h.8w2v8m7wviha"></a>14	<a name="h.o7cjy19yx1r9"></a>16
<a name="h.fssjekkfcw5"></a>Buffer	<a name="h.u9hn4z96wmog"></a>5	<a name="h.sgrbfqcsvbbo"></a>5
<a name="h.a7o8eoqij20v"></a>E	<a name="h.p0etf680n5jj"></a>0	<a name="h.1gfz3kwpzqb6"></a>0
<a name="h.vjzfen6sbuwt"></a>X	<a name="h.kcj15mi4aeut"></a>2.5	<a name="h.8v412vgf19w"></a>0
<a name="h.drpk3mbv39qo"></a>S	<a name="h.hvia2xanyz3d"></a>0	<a name="h.k2aq61tqtj3y"></a>2.5
<a name="h.i2kc1ieqocsh"></a>P	<a name="h.2uo933r4b8v2"></a>2.5	<a name="h.l3vs5rjbh12u"></a>2.5
<a name="h.k8vukgqc6e9m"></a>Water	<a name="h.ukbjar9e8ffp"></a>26	<a name="h.3dcxfs6p2top"></a>24

<a name="h.ha9gd621wus0"></a>Picking colonies

<a name="h.mnqycw5pabs5"></a>i5-49-5: 2 colonies i5-50-1, i5-50-2

<a name="h.ayr4ugq2sf4s"></a>i5-49-6: 1 colonies i5-50-3

<a name="h.mg228z8xx7fh"></a>Gel extraction

<a name="h.ywr7reggkz4f"></a>i5-50-1 ComB → 14.5

<a name="h.q6znc7yp6lb"></a>i5-50-2 ComC → 25.8

<a name="h.ynt0199bjmol"></a>8/26

<a name="h.vwa77gh8p8hf"></a>PCR

<a name="h.bjtev2ev8kec"></a>DNA i5-12-1

<a name="h.jymnywsnzftg"></a>F primer i5-10-10

<a name="h.sor7zpvo1nyg"></a>Reverse primer i5-7-10

<a name="h.nnjosx6c953"></a>	<a name="h.mtrb3iva0it0"></a>DEX
<a name="h.2axkjrqujymf"></a>G5	<a name="h.gnl6tjyvfu67"></a>25
<a name="h.exw1v9jv8rwt"></a>Primer F	<a name="h.vnfm7cumds74"></a>2.5
<a name="h.20mo9g2t0ts2"></a>Reverse	<a name="h.xoxs64p5vs1"></a>2.5
<a name="h.d3b19h8f6syn"></a>DNA	<a name="h.6zg4qvl4ud81"></a>5
<a name="h.3c8s3vxykvpt"></a>Water	<a name="h.4gc4v09aziij"></a>15
<a name="h.pjtvrbhao4l1"></a>Total	<a name="h.wbj7jxrujxto"></a>50

<a name="h.7kelk7kp46pk"></a>Glycerol Stock

<a name="h.uumk1ldo0ask"></a>i5-50-1 → 35.6

<a name="h.r6m1zi81uk0u"></a>i5-50-2 → 33.2

<a name="h.xv5rofx7ede6"></a>i5-50-3 → 103.2

<a name="h.8rzq6uem2bhv"></a>Miniprep

<a name="h.w8wrw9n4p3fr"></a>i5-50-1 → 35.6

<a name="h.vhot6id0l7lx"></a>i5-50-2 → 33.2

<a name="h.1td2t5tup2o2"></a>i5-50-3 → 103.2

<a name="h.p1rl4jcvl9mr"></a>Test Digestion

<a name="h.cvocygaovlgl"></a>	<a name="h.uaabmyzdxdoz"></a>i5-50-1	<a name="h.rdoglttqhd4"></a>i5-50-2
<a name="h.n12ylg36w345"></a>DNA	<a name="h.5jrd8hc43isx"></a>7	<a name="h.5pp98sgbef3p"></a>7
<a name="h.3fn8x6u1x7sy"></a>Buffer	<a name="h.63du1a6h1pr9"></a>2	<a name="h.oof408vnrdpz"></a>2
<a name="h.ijxh2t3aix43"></a>E	<a name="h.wtn9bfj3bkm0"></a>1	<a name="h.apqd8k3r9z72"></a>1
<a name="h.kt1psnx4c65"></a>P	<a name="h.t0cug9vmf5og"></a>1	<a name="h.tyw1cb560oxs"></a>1
<a name="h.jqywe4o71ph5"></a>Water	<a name="h.tyn20marrm8l"></a>9	<a name="h.4zm3e1uc9al0"></a>9
<a name="h.yv91lfqe7onv"></a>Total	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.hcq4nhhd3a3y"></a>20

<a name="h.mpptd6ozuq7t"></a>Mutagenesis #2

<a name="h.cvocygaovlgl"></a>	<a name="h.uaabmyzdxdoz"></a>i5-50-1	<a name="h.rdoglttqhd4"></a>i5-50-2
<a name="h.n12ylg36w345"></a>Q5	<a name="h.5jrd8hc43isx"></a>25	<a name="h.5pp98sgbef3p"></a>25
<a name="h.3fn8x6u1x7sy"></a>F - 4	<a name="h.63du1a6h1pr9"></a>1.1	<a name="h.oof408vnrdpz"></a>1.1
<a name="h.ijxh2t3aix43"></a>R - 4	<a name="h.wtn9bfj3bkm0"></a>1.1	<a name="h.apqd8k3r9z72"></a>1.1
<a name="h.kt1psnx4c65"></a>DNA	<a name="h.t0cug9vmf5og"></a>4	<a name="h.tyw1cb560oxs"></a>4
<a name="h.jqywe4o71ph5"></a>Water	<a name="h.tyn20marrm8l"></a>19	<a name="h.4zm3e1uc9al0"></a>19
<a name="h.yv91lfqe7onv"></a>Total	<a name="h.c9zf9yk69c7q"></a>50	<a name="h.hcq4nhhd3a3y"></a>50

<a name="h.wh82q8fzdx8i"></a>Ligation Com B and C combined insert : i5-50-1 vector: i5-50-2

<a name="h.cvocygaovlgl"></a>	<a name="h.uaabmyzdxdoz"></a>3:1	<a name="h.rdoglttqhd4"></a>0:1
<a name="h.n12ylg36w345"></a>Insert	<a name="h.5jrd8hc43isx"></a>4	<a name="h.5pp98sgbef3p"></a>0
<a name="h.3fn8x6u1x7sy"></a>Vector	<a name="h.63du1a6h1pr9"></a>2	<a name="h.oof408vnrdpz"></a>2
<a name="h.ijxh2t3aix43"></a>Buffer	<a name="h.wtn9bfj3bkm0"></a>2	<a name="h.apqd8k3r9z72"></a>2
<a name="h.kt1psnx4c65"></a>Ligase	<a name="h.t0cug9vmf5og"></a>1	<a name="h.tyw1cb560oxs"></a>1
<a name="h.jqywe4o71ph5"></a>Water	<a name="h.tyn20marrm8l"></a>11	<a name="h.4zm3e1uc9al0"></a>15
<a name="h.yv91lfqe7onv"></a>Total	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.hcq4nhhd3a3y"></a>20

<a name="h.68gouos0t8on"></a>Digestion

<a name="h.cvocygaovlgl"></a>	<a name="h.uaabmyzdxdoz"></a>i5-50-4	<a name="h.uaabmyzdxdoz"></a>Unknown	<a name="h.rdoglttqhd4"></a>i5-11-5
<a name="h.n12ylg36w345"></a>DNA	<a name="h.5jrd8hc43isx"></a>30	<a name="h.5jrd8hc43isx"></a>5	<a name="h.5pp98sgbef3p"></a>15
<a name="h.3fn8x6u1x7sy"></a>Buffer	<a name="h.63du1a6h1pr9"></a>5	<a name="h.63du1a6h1pr9"></a>2	<a name="h.oof408vnrdpz"></a>5
<a name="h.ijxh2t3aix43"></a>E	<a name="h.wtn9bfj3bkm0"></a>0	<a name="h.wtn9bfj3bkm0"></a>1	<a name="h.apqd8k3r9z72"></a>0
<a name="h.kt1psnx4c65"></a>X	<a name="h.t0cug9vmf5og"></a>1	<a name="h.t0cug9vmf5og"></a>0	<a name="h.tyw1cb560oxs"></a>1
<a name="h.jqywe4o71ph5"></a>S	<a name="h.tyn20marrm8l"></a>1	<a name="h.tyn20marrm8l"></a>0	<a name="h.4zm3e1uc9al0"></a>1
<a name="h.yv91lfqe7onv"></a>Water	<a name="h.c9zf9yk69c7q"></a>13	<a name="h.c9zf9yk69c7q"></a>12	<a name="h.hcq4nhhd3a3y"></a>28
<a name="h.yv91lfqe7onv"></a>Total	<a name="h.c9zf9yk69c7q"></a>50	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.hcq4nhhd3a3y"></a>50

<a name="h.fdk7q9mba35o"></a>These became i5 - 51 - 1/2/3

<a name="h.d61fs12b3you"></a>I5-51-3 was dephosphorylated

<a name="h.bsn9fco33fm3"></a>Transformation

<a name="h.lkvxwkpi9inq"></a>I5-52-1 and 2 . Zero colonies due to incorrect antibiotic

<a name="h.dvzb3eumrern"></a>I5-50-1 Became I5-52-3 and had zero colonies

<a name="h.kpi3hgrkk06a"></a>I5-50-2 became I5-52-4 and had 3 colonies which were picked and became I5 - 52 - 5/6

<a name="h.o2qd218hcvia"></a>8/28

<a name="h.z7xzj4gx195z"></a>Ligation of Dex and “Vector”

<a name="h.cvocygaovlgl"></a>	<a name="h.uaabmyzdxdoz"></a>1:1	<a name="h.uaabmyzdxdoz"></a>2:1	<a name="h.uaabmyzdxdoz"></a>0:1	<a name="h.rdoglttqhd4"></a>1:0
<a name="h.n12ylg36w345"></a>Dex	<a name="h.5jrd8hc43isx"></a>4	<a name="h.5jrd8hc43isx"></a>4	<a name="h.5jrd8hc43isx"></a>4	<a name="h.5pp98sgbef3p"></a>0
<a name="h.3fn8x6u1x7sy"></a>Vector	<a name="h.63du1a6h1pr9"></a>2.5	<a name="h.63du1a6h1pr9"></a>5	<a name="h.63du1a6h1pr9"></a>0	<a name="h.oof408vnrdpz"></a>2.5
<a name="h.ijxh2t3aix43"></a>Buffer	<a name="h.wtn9bfj3bkm0"></a>2	<a name="h.wtn9bfj3bkm0"></a>2	<a name="h.wtn9bfj3bkm0"></a>2	<a name="h.apqd8k3r9z72"></a>2
<a name="h.kt1psnx4c65"></a>Ligase	<a name="h.t0cug9vmf5og"></a>1	<a name="h.t0cug9vmf5og"></a>1	<a name="h.t0cug9vmf5og"></a>1	<a name="h.tyw1cb560oxs"></a>1
<a name="h.jqywe4o71ph5"></a>Water	<a name="h.tyn20marrm8l"></a>10.5	<a name="h.tyn20marrm8l"></a>8	<a name="h.tyn20marrm8l"></a>13	<a name="h.4zm3e1uc9al0"></a>14.5
<a name="h.yv91lfqe7onv"></a>Total	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.hcq4nhhd3a3y"></a>20

<a name="h.yq1mt5ngoaik"></a>Glycerol stock and miniprep

<a name="h.bwfe5kefh6yq"></a>I5 - 52 -5

<a name="h.mhtj9jx85y47"></a>I5 - 52 - 6

<a name="h.11290m459lpi"></a>Test DIgestion

<a name="h.cvocygaovlgl"></a>	<a name="h.uaabmyzdxdoz"></a>3I5 - 52 - 5	<a name="h.rdoglttqhd4"></a>I5 - 52 - 6
<a name="h.n12ylg36w345"></a>DNA	<a name="h.5jrd8hc43isx"></a>2	<a name="h.5pp98sgbef3p"></a>2
<a name="h.3fn8x6u1x7sy"></a>Buffer	<a name="h.63du1a6h1pr9"></a>2	<a name="h.oof408vnrdpz"></a>2
<a name="h.ijxh2t3aix43"></a>P	<a name="h.wtn9bfj3bkm0"></a>1	<a name="h.apqd8k3r9z72"></a>1
<a name="h.jqywe4o71ph5"></a>Water	<a name="h.tyn20marrm8l"></a>15	<a name="h.4zm3e1uc9al0"></a>15
<a name="h.yv91lfqe7onv"></a>Total	<a name="h.c9zf9yk69c7q"></a>20	<a name="h.hcq4nhhd3a3y"></a>20

<a name="h.fas6mntge4hv"></a>8/29

<a name="h.20xszn4oh76"></a>Replate

<a name="h.jigeqed11pyu"></a>I5 - 52 - 1 ----> 30+ colonies picked 2 I5 - 53 - 3 /4

<a name="h.4ysas0nwzukk"></a>I5 - 52 - 2 -----> 2 colonies

<a name="h.al5641i72xkl"></a>+ control ----> to many to count

<a name="h.2uyflmn7hzph"></a>2:1 ----> 6 colonies picked 2 I5 - 53 - 1 /2

<a name="h.fhckggg7zx9a"></a>1:1 ----> 5 colonies picked 1 I5 - 53 - 3

<a name="h.88b9a4mqw4u2"></a>0:1 ----> 0 colonies

<a name="h.ia4hh2fc1wiw"></a>1:0 ----> 0 colonies

<a name="h.w1mqbcgc9x04"></a>8/30

<a name="h.t9s8v0s3mrbt"></a>Miniprep & glycerol stock

<a name="h.tr8obmqxvn8d"></a>i5-53-1 → 108.9 (Dex)

<a name="h.z6eevbn2vu48"></a>i5-53-2 → 68.2

<a name="h.7mbw71lwms"></a>i5-53-3 → 116.9

<a name="h.j97ebnmhdns"></a>i5-53-4 → 161.7

<a name="h.9l17k1vdd687"></a>i5-53-5 → 146.7

<a name="h.2xg96aqk56yf"></a>Test Digestion

<a name="h.jzxenyw7z10t"></a>	<a name="h.4dsjfbhfrt2d"></a>i5-53-1	<a name="h.hpqskj2yh0ls"></a>	<a name="h.sdeeyuuas1kr"></a>i5-53-2	<a name="h.z35qqmds4j7b"></a>	<a name="h.xsrdkm6arlzc"></a>i5-53-1
<a name="h.8g8oecg33y1h"></a>DNA	<a name="h.fsvgzvsveyp5"></a>3	<a name="h.u4x4pwb32xyh"></a>3	<a name="h.64o3enmaumpx"></a>4	<a name="h.p8gha1wi5ftz"></a>4	<a name="h.ble4gjdleyrh"></a>3
<a name="h.5judswqx5yh1"></a>Buffer	<a name="h.9leaszgnui47"></a>2	<a name="h.r3cg8c5fbcdj"></a>2	<a name="h.1f4bcq5tl6y9"></a>2	<a name="h.q5101progiyp"></a>2	<a name="h.7yzz4x74c4ur"></a>2
<a name="h.z52ftdeypach"></a>E	<a name="h.ulqqywcuqi1r"></a>0	<a name="h.uzp2wjjw6jtb"></a>1	<a name="h.1cip9iacbrxz"></a>0	<a name="h.zh9gl3vu2odv"></a>0	<a name="h.2iinh1xhd4x2"></a>0
<a name="h.ro9dtybg9nox"></a>X	<a name="h.icx0i2i8duw9"></a>1	<a name="h.2lxgr2dz6cmo"></a>0	<a name="h.qvqcosvr4vcs"></a>1	<a name="h.txtb1uhfaq1t"></a>0	<a name="h.kjtybjtl8v3l"></a>0
<a name="h.sij17ar8gzgs"></a>S	<a name="h.854p4hypd9g"></a>1	<a name="h.uf87lbnoq2pi"></a>0	<a name="h.lew0bb7boo9p"></a>1	<a name="h.iq7b48no3h3o"></a>0	<a name="h.lq82ityimcwu"></a>0
<a name="h.ok6cwya4xpni"></a>P	<a name="h.l8r57jfbyf1k"></a>0	<a name="h.nqoqcwja3ru"></a>0	<a name="h.ou251ggef9qd"></a>0	<a name="h.4aemxrr9k3s"></a>1	<a name="h.74t2rwnwd4yd"></a>1
<a name="h.a1ie3z3bl7lz"></a>Water	<a name="h.wln2xyrkqjfx"></a>13	<a name="h.qjzd2k2bva2r"></a>14	<a name="h.ro1sy7xs7j7w"></a>12	<a name="h.eu4fw6737bny"></a>13	<a name="h.32ls6ya5pqye"></a>14
<a name="h.8tn5qvylt28q"></a>	<a name="h.5lx3npuz2iy7"></a>1	<a name="h.3plt6owxzhh0"></a>2	<a name="h.fnv7k6itrst2"></a>4	<a name="h.dnapl3ccjii6"></a>5	<a name="h.q81jqb7i1qs3"></a>3

<a name="h.fcspzxtte3b"></a>Test Digestion

<a name="h.eeyov5jivxat"></a>	<a name="h.kl7kbbseapg"></a>i5-53-4	<a name="h.s41l5rmf0bs4"></a>i5-53-5
<a name="h.fltgkpshrcbs"></a>DNA	<a name="h.b98jpscwqwbk"></a>4	<a name="h.aiqojitkw7vg"></a>4
<a name="h.x6xp5der2ug6"></a>Buffer	<a name="h.3gqf5wvsnyaw"></a>2	<a name="h.bb0mvrqei4rj"></a>2
<a name="h.8tcc2l1ta2d5"></a>P	<a name="h.dpcty77txhfk"></a>12	<a name="h.rw0lqg7rrew3"></a>12
<a name="h.us4t3thw066u"></a>Water	<a name="h.luk7ndt8o8j1"></a>1	<a name="h.z1rrnjiz6k0h"></a>1
<a name="h.ywqcom2kyyz8"></a>Total	<a name="h.mubupcvrfmz8"></a>20	<a name="h.rycyzuyt9e8t"></a>20

<a name="h.u4fh26gjgad5"></a>Test Digestion

<a name="h.rs3sueh9t6ge"></a>	<a name="h.t1je2tbxp68i"></a>i5-52-5	<a name="h.sfqg13l4zbcp"></a>i5-52-6
<a name="h.hz6jkau4rfs6"></a>DNA	<a name="h.oimc6tdk15jl"></a>4	<a name="h.5v3pzda74g8r"></a>4
<a name="h.3ijw5h97ghxu"></a>Buffer	<a name="h.bzlfbrbu9tnj"></a>2	<a name="h.eo2dmvwtebsg"></a>2
<a name="h.ye9tnj8vo3cp"></a>Water	<a name="h.x1keh7vo5pkv"></a>13	<a name="h.ugqtocesucsy"></a>13
<a name="h.93ewrgz4fbjg"></a>P	<a name="h.3vv55o7v7onu"></a>1	<a name="h.mqmyn3aias9t"></a>1
<a name="h.3jz94te8z8mv"></a>Total	<a name="h.svrvg9osp1eh"></a>20	<a name="h.ebqv9yeqx72u"></a>20

Revision as of 21:06, 23 September 2013 (view source) Alecspin (Talk \| contribs) (Replaced content with "Doesn't exist yet") ← Older edit		Latest revision as of 02:08, 28 September 2013 (view source) Alecspin (Talk \| contribs)
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-	~~Doesn't exist yet~~	+	<p class="c0 c11"><a name="h.ofxdus4hkgqb"></a></p><p class="c0"><a name="h.mdl7xxxdjt4h"></a><span>8/23</span></p><p class="c0"><a name="h.5xsqt7ge99ee"></a><span>Miniprep/nanodrop</span></p><p class="c0"><a name="h.ctzr6b52iix8"></a><span>i5-45-2 ComA → i5-46-14 → 23.4 ng/ul </span></p><p class="c0"><a name="h.gja1o1xcsept"></a><span>i5-45-3 ComA → i5-46-15</span></p><p class="c0 c11"><a name="h.a2hhtku9i6io"></a></p><p class="c0"><a name="h.c02sd7dnsuxi"></a><span>i5-43-12 (CP- Scr-pSur - RBS-GFP) → i5-46-16 → 250 ng/ul</span></p><p class="c0 c11"><a name="h.yfcv88m0stw2"></a></p><p class="c0"><a name="h.v826cclo51xq"></a><span>i5-11-5 →129.8</span></p><p class="c0"><a name="h.a133mmz89cfs"></a><span>i5-11-5 →129.9</span></p><p class="c0"><a name="h.m6kr3jcqty82"></a><span>i5-11-5 →157.6</span></p><p class="c0"><a name="h.wpg2no967d3i"></a><span>i5-11-5 →125.4</span></p><p class="c0"><a name="h.ylsjxgz2umn1"></a><span>i5-11-5 →112.5</span></p><p class="c0 c11"><a name="h.rvzpbptm1ezc"></a></p><p class="c0"><a name="h.w0dwoegdqnoo"></a><span>Test Digestion </span></p><p class="c0"><a name="h.utwwqkctwand"></a><span>E/P used</span></p><p class="c0 c11"><a name="h.ayylrj3mzles"></a></p><a href="#" name="13743df1e47439cebe0d57b03ca33d93feaf0ea9"></a><a href="#" name="79"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c2"><p class="c0 c11 c3"><a name="h.4jvmna5jr96h"></a></p></td><td class="c2"><p class="c0 c3"><a name="h.w6isy7n1z7ha"></a><span>i5-46-14</span></p></td><td class="c2"><p class="c0 c3"><a name="h.qma88sakg5g"></a><span>i5-46-15</span></p></td><td class="c2"><p class="c0 c3"><a name="h.af2dhot2q19u"></a><span>i5-46-16</span></p></td></tr><tr><td class="c2"><p class="c0 c3"><a name="h.mweie01wos47"></a><span>DNA</span></p></td><td class="c2"><p class="c0 c3"><a name="h.x7agldda9we4"></a><span>10</span></p></td><td class="c2"><p class="c0 c3"><a name="h.45ua2a67k5ak"></a><span>11</span></p></td><td class="c2"><p class="c0 c3"><a name="h.gjmx3d51vw6d"></a><span>1</span></p></td></tr><tr><td class="c2"><p class="c0 c3"><a name="h.20zvhl9aedd3"></a><span>Buffer</span></p></td><td class="c2"><p class="c0 c3"><a name="h.ceih3pa3mra4"></a><span>2</span></p></td><td class="c2"><p class="c0 c3"><a name="h.j00xod57omxh"></a><span>2</span></p></td><td class="c2"><p class="c0 c3"><a name="h.1js16x5pnmwb"></a><span>2</span></p></td></tr><tr><td class="c2"><p class="c0 c3"><a name="h.uusqov85j404"></a><span>E</span></p></td><td class="c2"><p class="c0 c3"><a name="h.9rw52ce0ezcv"></a><span>0</span></p></td><td class="c2"><p class="c0 c3"><a name="h.e02k9fcsbmmu"></a><span>0</span></p></td><td class="c2"><p class="c0 c3"><a name="h.ijynz4yv0qah"></a><span>1</span></p></td></tr><tr><td class="c2"><p class="c0 c3"><a name="h.ux6fp0ay10jy"></a><span>P</span></p></td><td class="c2"><p class="c0 c3"><a name="h.m1cymlp4oruj"></a><span>1</span></p></td><td class="c2"><p class="c0 c3"><a name="h.hlb3ljrx8b46"></a><span>1</span></p></td><td class="c2"><p class="c0 c3"><a name="h.grt85umlt6kt"></a><span>1</span></p></td></tr><tr><td class="c2"><p class="c0 c3"><a name="h.l5srj41q69np"></a><span>Water</span></p></td><td class="c2"><p class="c0 c3"><a name="h.7i29fh4kdg3"></a><span>7</span></p></td><td class="c2"><p class="c0 c3"><a name="h.xjal23kcxves"></a><span>6</span></p></td><td class="c2"><p class="c0 c3"><a name="h.slues9am83vc"></a><span>15</span></p></td></tr><tr><td class="c2"><p class="c0 c3"><a name="h.ai4na2btkv19"></a><span>Total</span></p></td><td class="c2"><p class="c0 c3"><a name="h.ghbiqqy43fx"></a><span>20</span></p></td><td class="c2"><p class="c0 c3"><a name="h.6sdztehr1ah5"></a><span>20</span></p></td><td class="c2"><p class="c0 c3"><a name="h.sin6a82e27eb"></a><span>20</span></p></td></tr><tr><td class="c2"><p class="c0 c11 c3"><a name="h.a3fhmuu8alye"></a></p></td><td class="c2"><p class="c0 c3"><a name="h.u12h1jpzx0g2"></a><span class="c13">1</span></p></td><td class="c2"><p class="c0 c3"><a name="h.g4y0epj01up4"></a><span class="c13">2</span></p></td><td class="c2"><p class="c0 c3"><a name="h.fe0gi7ev1hhd"></a><span class="c13">3</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.loc71y9hu8so"></a></p><p class="c0"><a name="h.hjb6jkt8i9j6"></a><span>Picked from glycerol stock </span></p><p class="c0"><a name="h.e2mgxko1y8we"></a><span>i5-47-1 ComB → from i5-22-1</span></p><p class="c0"><a name="h.dsgka111eja8"></a><span>i5-47-2 Com C → from i5-22-4</span></p><p class="c0 c11"><a name="h.grx4fxl896qc"></a></p><p class="c0"><a name="h.xrujmyk02uc0"></a><span>i5-22-5 Scr</span></p><p class="c0"><a name="h.bw8baiv3ogae"></a><span>i5-22-6 pFru</span></p><p class="c0 c11"><a name="h.4664ixq2wmon"></a></p><p class="c0"><a name="h.u4i83vrwug3b"></a><span>8/24</span></p><p class="c0"><a name="h.ptn8jdlrr7w3"></a><span>Miniprep and Nanodrop</span></p><p class="c0"><a name="h.tmxjgtc0kp2l"></a><span>i5-46-10 → 68.9</span></p><p class="c0"><a name="h.cnp2rwemrkux"></a><span>i5-46-11→ 40.4</span></p><p class="c0"><a name="h.hgyx1mskxf1w"></a><span>i5-46-1 → 198.2</span></p><p class="c0"><a name="h.2fo4ma7ic9b0"></a><span>i5-46-2 → 160.1</span></p><p class="c0 c11"><a name="h.x3xqsmwotbr2"></a></p><p class="c0"><a name="h.fonkqhabrm56"></a><span>ComA mutagenesis #1 (attempt 2)</span></p><p class="c0 c11"><a name="h.sikgxiwscv5"></a></p><a href="#" name="416c308b3c7a057b9d1019e0b06e4a9b724f69cb"></a><a href="#" name="80"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c39"><p class="c0 c11 c3"><a name="h.eajl4vssk8vz"></a></p></td><td class="c39"><p class="c0 c3"><a name="h.cq4v0p75fhn4"></a><span>i5-40-1</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.eu841w90i5ee"></a><span>QS</span></p></td><td class="c39"><p class="c0 c3"><a name="h.zjvg2gl8bkj"></a><span>25</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.jc8jzw6rg3dt"></a><span>F-primer</span></p></td><td class="c39"><p class="c0 c3"><a name="h.quc8laf5n2wn"></a><span>1.1</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.t73em28z47jq"></a><span>R-primer</span></p></td><td class="c39"><p class="c0 c3"><a name="h.28dhcswukz7r"></a><span>1.1</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.o31epurx3w35"></a><span>DNA</span></p></td><td class="c39"><p class="c0 c3"><a name="h.2k1sml5dv115"></a><span>1</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.yq3kvf6ecuau"></a><span>Water</span></p></td><td class="c39"><p class="c0 c3"><a name="h.mq838o83wvu8"></a><span>22</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.7lyxy4jpewm3"></a><span>Total</span></p></td><td class="c39"><p class="c0 c3"><a name="h.mzc8i2vwrc2l"></a><span>50</span></p></td></tr><tr><td class="c39"><p class="c0 c11 c3"><a name="h.790r9g43m7b0"></a></p></td><td class="c39"><p class="c0 c3"><a name="h.nam1h6z4oqhk"></a><span>i5-48-1</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.p82knvv8sdwl"></a></p><p class="c0"><a name="h.opisrjjglaut"></a><span>8/24</span></p><p class="c0"><a name="h.9fkso0reaxid"></a><span>Test Digestion </span></p><p class="c0 c11"><a name="h.s0qmtglz8cq0"></a></p><a href="#" name="7e2b33290663b04596aead84630932ec36e19769"></a><a href="#" name="81"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c1"><p class="c0 c11 c3"><a name="h.av8bh9leadg2"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.937o07p46yh0"></a><span>i5-46-10</span></p></td><td class="c1"><p class="c0 c3"><a name="h.qoa3kq48ld9s"></a><span>i5-46-11</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.lxxzdnyedh0v"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5tiy5wjwas98"></a><span>4.5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tpd8h8ot67u0"></a><span>7.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.tof73lpt01uh"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.f3n4bu52grue"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.o3xwyz9lhwsr"></a><span>2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.aze4k6hm3f2c"></a><span>X</span></p></td><td class="c1"><p class="c0 c3"><a name="h.79sa6kc0xyw"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.q0hax18eg4og"></a><span>2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.amtl2yc5gtdk"></a><span>S</span></p></td><td class="c1"><p class="c0 c3"><a name="h.2kv0rbtkabhj"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.bey9n683gs87"></a><span>2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.j7vqnmqzj303"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.at8k5b7e9nmc"></a><span>9.5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.75ot1094hg26"></a><span>6.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.626j8f7huchj"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.opk0dtqcu2er"></a><span>20</span></p></td><td class="c1"><p class="c0 c3"><a name="h.o9cttmwxa8i9"></a><span>20</span></p></td></tr><tr><td class="c1"><p class="c0 c11 c3"><a name="h.apfttnkfbz1x"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.6qonn7toamjb"></a><span class="c13">1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.gdvxojmpb711"></a><span class="c13">2</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.xi0g8dwsxrsg"></a></p><a href="#" name="b93987e1c461f5e138836bfb6a3216f1522eb426"></a><a href="#" name="82"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c1"><p class="c0 c11 c3"><a name="h.5oi3ljbgr3mj"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.oaerhkteii3o"></a><span>-5-46-20</span></p></td><td class="c1"><p class="c0 c3"><a name="h.ww2lyic5v41z"></a><span>15-46-11</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.o0qt8dt13d4n"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.oay07o87p1k9"></a><span>4.5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.t52aw9ml8nn9"></a><span>7.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.6vyfe3lnvbhb"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.xuifyolcredz"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.vnkvn7dxjueh"></a><span>2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.rhrll65pumag"></a><span>E</span></p></td><td class="c1"><p class="c0 c3"><a name="h.8iumcsv4f9c9"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.l0kksl5wcpgp"></a><span>2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.20im5rrj82mx"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.kcvh9bujka92"></a><span>11.5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.nfemvspw05pr"></a><span>8.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.vmj0rt4ol9q6"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.9ozg7f4aizjs"></a><span>20</span></p></td><td class="c1"><p class="c0 c3"><a name="h.uzy9t4lyti1j"></a><span>20</span></p></td></tr><tr><td class="c1"><p class="c0 c11 c3"><a name="h.8hlsu9obui5o"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.1eidt6ph9hhi"></a><span class="c13">3</span></p></td><td class="c1"><p class="c0 c3"><a name="h.8isovkrkpecs"></a><span class="c13">4</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.4mo59qkgcqx0"></a></p><p class="c0"><a name="h.8bmlbu8dgtkd"></a><span>Digestion </span></p><p class="c0 c11"><a name="h.4h1imecvc2ux"></a></p><a href="#" name="095f513337f59b77a9c61af718ba1cc74cecfa8b"></a><a href="#" name="83"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c1"><p class="c0 c11 c3"><a name="h.g6359lfayv0o"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.xedyysfsnvi2"></a><span>i5-47-1 ComB</span></p></td><td class="c1"><p class="c0 c3"><a name="h.wmwgz93pllb4"></a><span>i5-47-2 ComC</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.y88xro578pw7"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.noacn9y9ei3o"></a><span>10</span></p></td><td class="c1"><p class="c0 c3"><a name="h.jzvx5h6yrbku"></a><span>12.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.v41g7kbsny7f"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.3iotgafuvcim"></a><span>5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.cwh670qlrx6t"></a><span>5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.sy2g5omkqw51"></a><span>E</span></p></td><td class="c1"><p class="c0 c3"><a name="h.27djc45aaoqy"></a><span>0</span></p></td><td class="c1"><p class="c0 c3"><a name="h.xcllte99hz9i"></a><span>0</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.45c20xmk97kl"></a><span>X</span></p></td><td class="c1"><p class="c0 c3"><a name="h.a1w7vywdfjw1"></a><span>1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.zed4jb1ehc6d"></a><span>0</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.qr9m788dha7q"></a><span>S</span></p></td><td class="c1"><p class="c0 c3"><a name="h.28mooqcl9msj"></a><span>0</span></p></td><td class="c1"><p class="c0 c3"><a name="h.cjvi5jxli9o1"></a><span>1</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.kglnxw624vxq"></a><span>P</span></p></td><td class="c1"><p class="c0 c3"><a name="h.g66qdacedbt1"></a><span>1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.7p6us0h2mchy"></a><span>1</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.c9ysqtjz68es"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.sxyrab5wg1w0"></a><span>33</span></p></td><td class="c1"><p class="c0 c3"><a name="h.52asrxxrsaz9"></a><span>30.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.4hiyrvjph74t"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.s4od6vlql4jg"></a><span>50</span></p></td><td class="c1"><p class="c0 c3"><a name="h.6ez910a6d9nk"></a><span>50</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.92fiyo219boc"></a></p><p class="c0"><a name="h.q4yq7ejlqme2"></a><span>Glycerol stock </span></p><p class="c0"><a name="h.hk79umej219x"></a><span>made from i5-46-12, i5-46-13</span></p><p class="c0 c11"><a name="h.8bilibs5sol8"></a></p><p class="c0"><a name="h.qucz63u7f4un"></a><span>Miniprep/nanodrop </span></p><p class="c0"><a name="h.jglx03ebwusl"></a><span>i5-22-5 (ScR) → i5-49-1 → 151.3</span></p><p class="c0"><a name="h.4faac6oo6m92"></a><span>i5-22-6 (pFru) → i5-49-2 → 243.3</span></p><p class="c0"><a name="h.wa97pgbvpzfj"></a><span>i5-46-12 (CP-Cra-pFru-RBS-GFP) → i5-49-3 → 144.5</span></p><p class="c0"><a name="h.4dvg4275wfg9"></a><span>i5-46-13 (CP-ScR-pFru-RBS-GFP) → i5-49-4 → 294.8</span></p><p class="c0 c11"><a name="h.5qdhtdva7v7r"></a></p><p class="c0"><a name="h.yi49iqrxpeyv"></a><span>Transformation </span></p><p class="c0"><a name="h.blp16xoar170"></a><span>i5-48-1 → i5-49-5 → 20 colonies</span></p><p class="c0"><a name="h.8hpm03ekc96b"></a><span>+ control (i5-11-5)</span></p><p class="c0"><a name="h.2tf6ooi2s6dj"></a><span>i5-46-16 → i5-49-6 → 30 colonies</span></p><p class="c0"><a name="h.wmc4rwad9ogm"></a><span>+ control (i5-11-5) → 150+ colonies</span></p><p class="c0 c11"><a name="h.eud8k4834cq8"></a></p><p class="c0"><a name="h.vofk4hpdzlfx"></a><span>Miniprep</span></p><p class="c0"><a name="h.rjlj715ycbc3"></a><span>i5-49-1 ComB → 150.7</span></p><p class="c0"><a name="h.xdprn312zwgg"></a><span>i5-49-2 ComC → 129.3</span></p><p class="c0 c11"><a name="h.brg61ziu1srk"></a></p><p class="c0"><a name="h.xj9616emb6zw"></a><span>Digestion </span></p><p class="c0 c11"><a name="h.7ub31y9l7an6"></a></p><a href="#" name="53a1828e0d2eacfa6aad16ee2f1898008b2ad0db"></a><a href="#" name="84"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c1"><p class="c0 c11 c3"><a name="h.crhm9o1ap5ia"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.qw10ikui1bss"></a><span>i5-49-1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.sdsmjcv45kyk"></a><span>i5-49-2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.7tnkjt38aq0f"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.8w2v8m7wviha"></a><span>14</span></p></td><td class="c1"><p class="c0 c3"><a name="h.o7cjy19yx1r9"></a><span>16</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.fssjekkfcw5"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.u9hn4z96wmog"></a><span>5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.sgrbfqcsvbbo"></a><span>5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.a7o8eoqij20v"></a><span>E</span></p></td><td class="c1"><p class="c0 c3"><a name="h.p0etf680n5jj"></a><span>0</span></p></td><td class="c1"><p class="c0 c3"><a name="h.1gfz3kwpzqb6"></a><span>0</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.vjzfen6sbuwt"></a><span>X</span></p></td><td class="c1"><p class="c0 c3"><a name="h.kcj15mi4aeut"></a><span>2.5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.8v412vgf19w"></a><span>0</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.drpk3mbv39qo"></a><span>S</span></p></td><td class="c1"><p class="c0 c3"><a name="h.hvia2xanyz3d"></a><span>0</span></p></td><td class="c1"><p class="c0 c3"><a name="h.k2aq61tqtj3y"></a><span>2.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.i2kc1ieqocsh"></a><span>P</span></p></td><td class="c1"><p class="c0 c3"><a name="h.2uo933r4b8v2"></a><span>2.5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.l3vs5rjbh12u"></a><span>2.5</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.k8vukgqc6e9m"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.ukbjar9e8ffp"></a><span>26</span></p></td><td class="c1"><p class="c0 c3"><a name="h.3dcxfs6p2top"></a><span>24</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.cw3vrm3ov790"></a></p><p class="c0"><a name="h.ha9gd621wus0"></a><span>Picking colonies</span></p><p class="c0"><a name="h.mnqycw5pabs5"></a><span>i5-49-5: 2 colonies i5-50-1, i5-50-2</span></p><p class="c0"><a name="h.ayr4ugq2sf4s"></a><span>i5-49-6: 1 colonies i5-50-3</span></p><p class="c0 c11"><a name="h.5uy8tygsoqeo"></a></p><p class="c0"><a name="h.mg228z8xx7fh"></a><span>Gel extraction </span></p><p class="c0"><a name="h.ywr7reggkz4f"></a><span>i5-50-1 ComB → 14.5</span></p><p class="c0"><a name="h.q6znc7yp6lb"></a><span>i5-50-2 ComC → 25.8</span></p><p class="c0 c11"><a name="h.ydg54m5p5sza"></a></p><p class="c0"><a name="h.ynt0199bjmol"></a><span>8/26</span></p><p class="c0"><a name="h.vwa77gh8p8hf"></a><span>PCR</span></p><p class="c0"><a name="h.bjtev2ev8kec"></a><span>DNA i5-12-1 </span></p><p class="c0"><a name="h.jymnywsnzftg"></a><span>F primer i5-10-10</span></p><p class="c0"><a name="h.sor7zpvo1nyg"></a><span>Reverse primer i5-7-10</span></p><p class="c0 c11"><a name="h.7iuidd3hg7m0"></a></p><a href="#" name="c5c4aef7df8a91b52ce699600c2ac0fa82fd414b"></a><a href="#" name="85"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c39"><p class="c0 c11 c3"><a name="h.nnjosx6c953"></a></p></td><td class="c39"><p class="c0 c3"><a name="h.mtrb3iva0it0"></a><span>DEX</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.2axkjrqujymf"></a><span>G5</span></p></td><td class="c39"><p class="c0 c3"><a name="h.gnl6tjyvfu67"></a><span>25</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.exw1v9jv8rwt"></a><span>Primer F</span></p></td><td class="c39"><p class="c0 c3"><a name="h.vnfm7cumds74"></a><span>2.5</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.20mo9g2t0ts2"></a><span>Reverse</span></p></td><td class="c39"><p class="c0 c3"><a name="h.xoxs64p5vs1"></a><span>2.5</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.d3b19h8f6syn"></a><span>DNA</span></p></td><td class="c39"><p class="c0 c3"><a name="h.6zg4qvl4ud81"></a><span>5</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.3c8s3vxykvpt"></a><span>Water</span></p></td><td class="c39"><p class="c0 c3"><a name="h.4gc4v09aziij"></a><span>15</span></p></td></tr><tr><td class="c39"><p class="c0 c3"><a name="h.pjtvrbhao4l1"></a><span>Total</span></p></td><td class="c39"><p class="c0 c3"><a name="h.wbj7jxrujxto"></a><span>50</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.a4itktb0w8aq"></a></p><p class="c0"><a name="h.7kelk7kp46pk"></a><span>Glycerol Stock</span></p><p class="c0"><a name="h.uumk1ldo0ask"></a><span>i5-50-1 → 35.6</span></p><p class="c0"><a name="h.r6m1zi81uk0u"></a><span>i5-50-2 → 33.2</span></p><p class="c0"><a name="h.xv5rofx7ede6"></a><span>i5-50-3 → 103.2</span></p><p class="c0 c11"><a name="h.gtyoudjvcr6d"></a></p><p class="c0"><a name="h.8rzq6uem2bhv"></a><span>Miniprep</span></p><p class="c0"><a name="h.w8wrw9n4p3fr"></a><span>i5-50-1 → 35.6</span></p><p class="c0"><a name="h.vhot6id0l7lx"></a><span>i5-50-2 → 33.2</span></p><p class="c0"><a name="h.1td2t5tup2o2"></a><span>i5-50-3 → 103.2</span></p><p class="c0 c11"><a name="h.fzrlvnz6z5vb"></a></p><p class="c0"><a name="h.p1rl4jcvl9mr"></a><span>Test Digestion </span></p><p class="c0 c11"><a name="h.ybnm5ncfmeus"></a></p><a href="#" name="2239632add60c199730e43beb39531084ee6f5b7"></a><a href="#" name="86"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c1"><p class="c0 c11 c3"><a name="h.cvocygaovlgl"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>i5-50-1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.rdoglttqhd4"></a><span>i5-50-2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.n12ylg36w345"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>7</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5pp98sgbef3p"></a><span>7</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.3fn8x6u1x7sy"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.oof408vnrdpz"></a><span>2</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.ijxh2t3aix43"></a><span>E</span></p></td><td class="c1"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.apqd8k3r9z72"></a><span>1</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.kt1psnx4c65"></a><span>P</span></p></td><td class="c1"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyw1cb560oxs"></a><span>1</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.jqywe4o71ph5"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>9</span></p></td><td class="c1"><p class="c0 c3"><a name="h.4zm3e1uc9al0"></a><span>9</span></p></td></tr><tr><td class="c1"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c1"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>20</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.rgeo2er9joo6"></a></p><p class="c0"><a name="h.mpptd6ozuq7t"></a><span>Mutagenesis #2</span></p><p class="c0 c11"><a name="h.rgr76ehar58m"></a></p><p class="c0 c11"><a name="h.ybnm5ncfmeus"></a></p><a href="#" name="334f667ce12180f29e8406a8cebd4936a23acd83"></a><a href="#" name="87"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr class="c14"><td class="c1"><p class="c0 c11 c3"><a name="h.cvocygaovlgl"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>i5-50-1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.rdoglttqhd4"></a><span>i5-50-2</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.n12ylg36w345"></a><span>Q5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>25</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5pp98sgbef3p"></a><span>25</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.3fn8x6u1x7sy"></a><span>F - 4</span></p></td><td class="c1"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>1.1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.oof408vnrdpz"></a><span>1.1</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.ijxh2t3aix43"></a><span>R - 4</span></p></td><td class="c1"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>1.1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.apqd8k3r9z72"></a><span>1.1</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.kt1psnx4c65"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>4</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyw1cb560oxs"></a><span>4</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.jqywe4o71ph5"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>19</span></p></td><td class="c1"><p class="c0 c3"><a name="h.4zm3e1uc9al0"></a><span>19</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>50</span></p></td><td class="c1"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>50</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.yeq53hog2nqz"></a></p><p class="c0 c11"><a name="h.5pst3suunqe9"></a></p><p class="c0 c11"><a name="h.qgn98zk3uxir"></a></p><p class="c0 c11"><a name="h.mrpy5zez3447"></a></p><p class="c0 c11"><a name="h.3314pw32qthd"></a></p><p class="c0 c11"><a name="h.wxv5wz8l0dth"></a></p><p class="c0 c11"><a name="h.wqfxnoxbgns7"></a></p><p class="c0 c11"><a name="h.u08g97e5ffcy"></a></p><p class="c0 c11"><a name="h.s9exftplvlo2"></a></p><p class="c0 c11"><a name="h.gyf247lct3x6"></a></p><p class="c0 c11"><a name="h.6tu3yvd83bn"></a></p><p class="c0"><a name="h.wh82q8fzdx8i"></a><span>Ligation Com B and C combined          insert : i5-50-1         vector:    i5-50-2</span></p><p class="c0 c11"><a name="h.ybnm5ncfmeus"></a></p><a href="#" name="5af96474f648af8b9d7bb9d783b3909d2e739aad"></a><a href="#" name="88"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr class="c14"><td class="c1"><p class="c0 c11 c3"><a name="h.cvocygaovlgl"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>3:1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.rdoglttqhd4"></a><span>0:1</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.n12ylg36w345"></a><span>Insert</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>4</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5pp98sgbef3p"></a><span>0</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.3fn8x6u1x7sy"></a><span>Vector</span></p></td><td class="c1"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.oof408vnrdpz"></a><span>2</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.ijxh2t3aix43"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.apqd8k3r9z72"></a><span>2</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.kt1psnx4c65"></a><span>Ligase</span></p></td><td class="c1"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyw1cb560oxs"></a><span>1</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.jqywe4o71ph5"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>11</span></p></td><td class="c1"><p class="c0 c3"><a name="h.4zm3e1uc9al0"></a><span>15</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c1"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>20</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.we08d2or53fg"></a></p><p class="c0 c11"><a name="h.ozewhdabftcc"></a></p><p class="c0"><a name="h.68gouos0t8on"></a><span>Digestion</span></p><p class="c0 c11"><a name="h.ybnm5ncfmeus"></a></p><a href="#" name="860552a604a3bb7d492846b67b9ba0e01623f04b"></a><a href="#" name="89"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr class="c14"><td class="c2"><p class="c0 c11 c3"><a name="h.cvocygaovlgl"></a></p></td><td class="c2"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>i5-50-4</span></p></td><td class="c2"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>Unknown</span></p></td><td class="c2"><p class="c0 c3"><a name="h.rdoglttqhd4"></a><span>i5-11-5</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.n12ylg36w345"></a><span>DNA</span></p></td><td class="c2"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>30</span></p></td><td class="c2"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>5</span></p></td><td class="c2"><p class="c0 c3"><a name="h.5pp98sgbef3p"></a><span>15</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.3fn8x6u1x7sy"></a><span>Buffer</span></p></td><td class="c2"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>5</span></p></td><td class="c2"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>2</span></p></td><td class="c2"><p class="c0 c3"><a name="h.oof408vnrdpz"></a><span>5</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.ijxh2t3aix43"></a><span>E</span></p></td><td class="c2"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>0</span></p></td><td class="c2"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>1</span></p></td><td class="c2"><p class="c0 c3"><a name="h.apqd8k3r9z72"></a><span>0</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.kt1psnx4c65"></a><span>X</span></p></td><td class="c2"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>1</span></p></td><td class="c2"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>0</span></p></td><td class="c2"><p class="c0 c3"><a name="h.tyw1cb560oxs"></a><span>1</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.jqywe4o71ph5"></a><span>S</span></p></td><td class="c2"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>1</span></p></td><td class="c2"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>0</span></p></td><td class="c2"><p class="c0 c3"><a name="h.4zm3e1uc9al0"></a><span>1</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Water</span></p></td><td class="c2"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>13</span></p></td><td class="c2"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>12</span></p></td><td class="c2"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>28</span></p></td></tr><tr class="c14"><td class="c2"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Total</span></p></td><td class="c2"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>50</span></p></td><td class="c2"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c2"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>50</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.kjfq9gjbiujs"></a></p><p class="c0"><a name="h.fdk7q9mba35o"></a><span>These became i5 - 51 - 1/2/3</span></p><p class="c0 c11"><a name="h.en21lf1rn796"></a></p><p class="c0"><a name="h.d61fs12b3you"></a><span>I5-51-3 was dephosphorylated</span></p><p class="c0 c11"><a name="h.7crsn0vrdwue"></a></p><p class="c0"><a name="h.bsn9fco33fm3"></a><span>Transformation</span></p><p class="c0 c11"><a name="h.vvaq8ntbzg3i"></a></p><p class="c0"><a name="h.lkvxwkpi9inq"></a><span>I5-52-1 and 2 . Zero colonies due to incorrect antibiotic </span></p><p class="c0 c11"><a name="h.e5wyfuiodoc9"></a></p><p class="c0"><a name="h.dvzb3eumrern"></a><span>I5-50-1 Became I5-52-3 and had zero colonies</span></p><p class="c0 c11"><a name="h.jqoc3u99toh3"></a></p><p class="c0"><a name="h.kpi3hgrkk06a"></a><span>I5-50-2 became I5-52-4 and had 3 colonies which were picked and became I5 - 52 - 5/6</span></p><p class="c0 c11"><a name="h.l0914cu38wdx"></a></p><p class="c0 c11"><a name="h.tk6bwjsesddi"></a></p><p class="c0 c11"><a name="h.gdnwx3i2ia62"></a></p><p class="c0"><a name="h.o2qd218hcvia"></a><span>8/28</span></p><p class="c0 c11"><a name="h.p8m5u5hzys9l"></a></p><p class="c0"><a name="h.z7xzj4gx195z"></a><span>Ligation of Dex and “Vector”</span></p><p class="c0 c11"><a name="h.gu1mntpjg4r9"></a></p><p class="c0 c11"><a name="h.ybnm5ncfmeus"></a></p><a href="#" name="45eb7ec7fb742c604d3901f81755fd04ca6288e5"></a><a href="#" name="90"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr class="c14"><td class="c20"><p class="c0 c11 c3"><a name="h.cvocygaovlgl"></a></p></td><td class="c20"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>1:1</span></p></td><td class="c20"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>2:1</span></p></td><td class="c20"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>0:1</span></p></td><td class="c20"><p class="c0 c3"><a name="h.rdoglttqhd4"></a><span>1:0</span></p></td></tr><tr class="c14"><td class="c20"><p class="c0 c3"><a name="h.n12ylg36w345"></a><span>Dex</span></p></td><td class="c20"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>4</span></p></td><td class="c20"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>4</span></p></td><td class="c20"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>4</span></p></td><td class="c20"><p class="c0 c3"><a name="h.5pp98sgbef3p"></a><span>0</span></p></td></tr><tr class="c14"><td class="c20"><p class="c0 c3"><a name="h.3fn8x6u1x7sy"></a><span>Vector</span></p></td><td class="c20"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>2.5</span></p></td><td class="c20"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>5</span></p></td><td class="c20"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>0</span></p></td><td class="c20"><p class="c0 c3"><a name="h.oof408vnrdpz"></a><span>2.5</span></p></td></tr><tr class="c14"><td class="c20"><p class="c0 c3"><a name="h.ijxh2t3aix43"></a><span>Buffer</span></p></td><td class="c20"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>2</span></p></td><td class="c20"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>2</span></p></td><td class="c20"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>2</span></p></td><td class="c20"><p class="c0 c3"><a name="h.apqd8k3r9z72"></a><span>2</span></p></td></tr><tr class="c14"><td class="c20"><p class="c0 c3"><a name="h.kt1psnx4c65"></a><span>Ligase</span></p></td><td class="c20"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>1</span></p></td><td class="c20"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>1</span></p></td><td class="c20"><p class="c0 c3"><a name="h.t0cug9vmf5og"></a><span>1</span></p></td><td class="c20"><p class="c0 c3"><a name="h.tyw1cb560oxs"></a><span>1</span></p></td></tr><tr class="c14"><td class="c20"><p class="c0 c3"><a name="h.jqywe4o71ph5"></a><span>Water</span></p></td><td class="c20"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>10.5</span></p></td><td class="c20"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>8</span></p></td><td class="c20"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>13</span></p></td><td class="c20"><p class="c0 c3"><a name="h.4zm3e1uc9al0"></a><span>14.5</span></p></td></tr><tr class="c14"><td class="c20"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Total</span></p></td><td class="c20"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c20"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c20"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c20"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>20</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.f3pxnco3ymqd"></a></p><p class="c0 c11"><a name="h.708mc4d4i6qu"></a></p><p class="c0 c11"><a name="h.f0b71c997997"></a></p><p class="c0"><a name="h.yq1mt5ngoaik"></a><span>Glycerol stock and miniprep</span></p><p class="c0 c11"><a name="h.q46lag701pia"></a></p><p class="c0"><a name="h.bwfe5kefh6yq"></a><span>I5 - 52 -5 </span></p><p class="c0 c11"><a name="h.kv7sreltktda"></a></p><p class="c0"><a name="h.mhtj9jx85y47"></a><span>I5 - 52 - 6</span></p><p class="c0 c11"><a name="h.du7nl7obuf7j"></a></p><p class="c0"><a name="h.11290m459lpi"></a><span>Test DIgestion</span></p><p class="c0 c11"><a name="h.cgzllw1w5xq"></a></p><p class="c0 c11"><a name="h.ybnm5ncfmeus"></a></p><a href="#" name="e8387e70a1e5080ab6530e879b7e300fceba6ccb"></a><a href="#" name="91"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr class="c14"><td class="c1"><p class="c0 c11 c3"><a name="h.cvocygaovlgl"></a></p></td><td class="c1"><p class="c0 c3"><a name="h.uaabmyzdxdoz"></a><span>3I5 - 52 - 5</span></p></td><td class="c1"><p class="c0 c3"><a name="h.rdoglttqhd4"></a><span>I5 - 52 - 6</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.n12ylg36w345"></a><span>DNA</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5jrd8hc43isx"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.5pp98sgbef3p"></a><span>2</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.3fn8x6u1x7sy"></a><span>Buffer</span></p></td><td class="c1"><p class="c0 c3"><a name="h.63du1a6h1pr9"></a><span>2</span></p></td><td class="c1"><p class="c0 c3"><a name="h.oof408vnrdpz"></a><span>2</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.ijxh2t3aix43"></a><span>P</span></p></td><td class="c1"><p class="c0 c3"><a name="h.wtn9bfj3bkm0"></a><span>1</span></p></td><td class="c1"><p class="c0 c3"><a name="h.apqd8k3r9z72"></a><span>1</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.jqywe4o71ph5"></a><span>Water</span></p></td><td class="c1"><p class="c0 c3"><a name="h.tyn20marrm8l"></a><span>15</span></p></td><td class="c1"><p class="c0 c3"><a name="h.4zm3e1uc9al0"></a><span>15</span></p></td></tr><tr class="c14"><td class="c1"><p class="c0 c3"><a name="h.yv91lfqe7onv"></a><span>Total</span></p></td><td class="c1"><p class="c0 c3"><a name="h.c9zf9yk69c7q"></a><span>20</span></p></td><td class="c1"><p class="c0 c3"><a name="h.hcq4nhhd3a3y"></a><span>20</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.we08d2or53fg"></a></p><p class="c0 c11"><a name="h.du7nl7obuf7j"></a></p><p class="c0"><a name="h.fas6mntge4hv"></a><span>8/29</span></p><p class="c0"><a name="h.20xszn4oh76"></a><span>Replate</span></p><p class="c0"><a name="h.jigeqed11pyu"></a><span>I5 - 52 - 1 ----> 30+ colonies picked 2     I5 - 53 - 3 /4  </span></p><p class="c0 c11"><a name="h.2oe5q5nbc11x"></a></p><p class="c0"><a name="h.4ysas0nwzukk"></a><span>I5 - 52 - 2 -----> 2 colonies</span></p><p class="c0 c11"><a name="h.bx8zv1rnjii2"></a></p><p class="c0"><a name="h.al5641i72xkl"></a><span>+ control ----> to many to count</span></p><p class="c0 c11"><a name="h.8w63ffh4lms7"></a></p><p class="c0"><a name="h.2uyflmn7hzph"></a><span>2:1 ----> 6 colonies picked 2 I5 - 53 - 1 /2</span></p><p class="c0"><a name="h.fhckggg7zx9a"></a><span>1:1 ----> 5 colonies picked 1 I5 - 53 - 3</span></p><p class="c0 c11"><a name="h.424e8xr5c3j3"></a></p><p class="c0"><a name="h.88b9a4mqw4u2"></a><span>0:1 ----> 0 colonies</span></p><p class="c0 c11"><a name="h.k86k14js456r"></a></p><p class="c0"><a name="h.ia4hh2fc1wiw"></a><span>1:0 ----> 0 colonies </span></p><p class="c0 c11"><a name="h.6serjwtg0q97"></a></p><p class="c0"><a name="h.w1mqbcgc9x04"></a><span>8/30</span></p><p class="c0"><a name="h.t9s8v0s3mrbt"></a><span>Miniprep & glycerol stock</span></p><p class="c0"><a name="h.tr8obmqxvn8d"></a><span>i5-53-1 → 108.9 (Dex)</span></p><p class="c0"><a name="h.z6eevbn2vu48"></a><span>i5-53-2 → 68.2</span></p><p class="c0"><a name="h.7mbw71lwms"></a><span>i5-53-3 → 116.9</span></p><p class="c0"><a name="h.j97ebnmhdns"></a><span>i5-53-4 → 161.7</span></p><p class="c0"><a name="h.9l17k1vdd687"></a><span>i5-53-5 → 146.7</span></p><p class="c0 c11"><a name="h.i29b7rs5ktjh"></a></p><p class="c0"><a name="h.2xg96aqk56yf"></a><span>Test Digestion</span></p><p class="c0 c11"><a name="h.z2toe2trnogj"></a></p><a href="#" name="db734728ea7823958e8acec9b6438780d17c7a06"></a><a href="#" name="92"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c5"><p class="c0"><a name="h.jzxenyw7z10t"></a><span> </span></p></td><td class="c5"><p class="c0"><a name="h.4dsjfbhfrt2d"></a><span>i5-53-1</span></p></td><td class="c5"><p class="c0"><a name="h.hpqskj2yh0ls"></a><span> </span></p></td><td class="c5"><p class="c0"><a name="h.sdeeyuuas1kr"></a><span>i5-53-2</span></p></td><td class="c5"><p class="c0"><a name="h.z35qqmds4j7b"></a><span> </span></p></td><td class="c5"><p class="c0"><a name="h.xsrdkm6arlzc"></a><span>i5-53-1</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.8g8oecg33y1h"></a><span>DNA</span></p></td><td class="c5"><p class="c0"><a name="h.fsvgzvsveyp5"></a><span>3</span></p></td><td class="c5"><p class="c0"><a name="h.u4x4pwb32xyh"></a><span>3</span></p></td><td class="c5"><p class="c0"><a name="h.64o3enmaumpx"></a><span>4</span></p></td><td class="c5"><p class="c0"><a name="h.p8gha1wi5ftz"></a><span>4</span></p></td><td class="c5"><p class="c0"><a name="h.ble4gjdleyrh"></a><span>3</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.5judswqx5yh1"></a><span>Buffer</span></p></td><td class="c5"><p class="c0"><a name="h.9leaszgnui47"></a><span>2</span></p></td><td class="c5"><p class="c0"><a name="h.r3cg8c5fbcdj"></a><span>2</span></p></td><td class="c5"><p class="c0"><a name="h.1f4bcq5tl6y9"></a><span>2</span></p></td><td class="c5"><p class="c0"><a name="h.q5101progiyp"></a><span>2</span></p></td><td class="c5"><p class="c0"><a name="h.7yzz4x74c4ur"></a><span>2</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.z52ftdeypach"></a><span>E</span></p></td><td class="c5"><p class="c0"><a name="h.ulqqywcuqi1r"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.uzp2wjjw6jtb"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.1cip9iacbrxz"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.zh9gl3vu2odv"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.2iinh1xhd4x2"></a><span>0</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.ro9dtybg9nox"></a><span>X</span></p></td><td class="c5"><p class="c0"><a name="h.icx0i2i8duw9"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.2lxgr2dz6cmo"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.qvqcosvr4vcs"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.txtb1uhfaq1t"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.kjtybjtl8v3l"></a><span>0</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.sij17ar8gzgs"></a><span>S</span></p></td><td class="c5"><p class="c0"><a name="h.854p4hypd9g"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.uf87lbnoq2pi"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.lew0bb7boo9p"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.iq7b48no3h3o"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.lq82ityimcwu"></a><span>0</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.ok6cwya4xpni"></a><span>P</span></p></td><td class="c5"><p class="c0"><a name="h.l8r57jfbyf1k"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.nqoqcwja3ru"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.ou251ggef9qd"></a><span>0</span></p></td><td class="c5"><p class="c0"><a name="h.4aemxrr9k3s"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.74t2rwnwd4yd"></a><span>1</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.a1ie3z3bl7lz"></a><span>Water</span></p></td><td class="c5"><p class="c0"><a name="h.wln2xyrkqjfx"></a><span>13</span></p></td><td class="c5"><p class="c0"><a name="h.qjzd2k2bva2r"></a><span>14</span></p></td><td class="c5"><p class="c0"><a name="h.ro1sy7xs7j7w"></a><span>12</span></p></td><td class="c5"><p class="c0"><a name="h.eu4fw6737bny"></a><span>13</span></p></td><td class="c5"><p class="c0"><a name="h.32ls6ya5pqye"></a><span>14</span></p></td></tr><tr class="c124"><td class="c5"><p class="c0"><a name="h.8tn5qvylt28q"></a><span> </span></p></td><td class="c5"><p class="c0"><a name="h.5lx3npuz2iy7"></a><span class="c13">1</span></p></td><td class="c5"><p class="c0"><a name="h.3plt6owxzhh0"></a><span class="c13">2</span></p></td><td class="c5"><p class="c0"><a name="h.fnv7k6itrst2"></a><span class="c13">4</span></p></td><td class="c5"><p class="c0"><a name="h.dnapl3ccjii6"></a><span class="c13">5</span></p></td><td class="c5"><p class="c0"><a name="h.q81jqb7i1qs3"></a><span class="c13">3</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.ok7suyh20en7"></a></p><p class="c0"><a name="h.fcspzxtte3b"></a><span>Test Digestion</span></p><p class="c0 c11"><a name="h.f25zts15wtip"></a></p><p class="c0 c11"><a name="h.eil90x9ur9wq"></a></p><a href="#" name="7979965d9edaf8c097c62c76e50da1c67c9f9ad0"></a><a href="#" name="93"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c5"><p class="c0"><a name="h.eeyov5jivxat"></a><span> </span></p></td><td class="c5"><p class="c0"><a name="h.kl7kbbseapg"></a><span>i5-53-4</span></p></td><td class="c5"><p class="c0"><a name="h.s41l5rmf0bs4"></a><span>i5-53-5</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.fltgkpshrcbs"></a><span>DNA</span></p></td><td class="c5"><p class="c0"><a name="h.b98jpscwqwbk"></a><span>4</span></p></td><td class="c5"><p class="c0"><a name="h.aiqojitkw7vg"></a><span>4</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.x6xp5der2ug6"></a><span>Buffer</span></p></td><td class="c5"><p class="c0"><a name="h.3gqf5wvsnyaw"></a><span>2</span></p></td><td class="c5"><p class="c0"><a name="h.bb0mvrqei4rj"></a><span>2</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.8tcc2l1ta2d5"></a><span>P</span></p></td><td class="c5"><p class="c0"><a name="h.dpcty77txhfk"></a><span>12</span></p></td><td class="c5"><p class="c0"><a name="h.rw0lqg7rrew3"></a><span>12</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.us4t3thw066u"></a><span>Water</span></p></td><td class="c5"><p class="c0"><a name="h.luk7ndt8o8j1"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.z1rrnjiz6k0h"></a><span>1</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.ywqcom2kyyz8"></a><span>Total</span></p></td><td class="c5"><p class="c0"><a name="h.mubupcvrfmz8"></a><span>20</span></p></td><td class="c5"><p class="c0"><a name="h.rycyzuyt9e8t"></a><span>20</span></p></td></tr></tbody></table><p class="c0 c11"><a name="h.48giyt3lzmsv"></a></p><p class="c0"><a name="h.u4fh26gjgad5"></a><span>Test Digestion</span></p><p class="c0 c11"><a name="h.ywhzlpyplasp"></a></p><a href="#" name="04c1b713c906ae6a2a6d104d10cd639dd3340b88"></a><a href="#" name="94"></a><table class="c6" cellpadding="0" cellspacing="0"><tbody><tr><td class="c5"><p class="c0"><a name="h.rs3sueh9t6ge"></a><span> </span></p></td><td class="c5"><p class="c0"><a name="h.t1je2tbxp68i"></a><span>i5-52-5</span></p></td><td class="c5"><p class="c0"><a name="h.sfqg13l4zbcp"></a><span>i5-52-6</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.hz6jkau4rfs6"></a><span>DNA</span></p></td><td class="c5"><p class="c0"><a name="h.oimc6tdk15jl"></a><span>4</span></p></td><td class="c5"><p class="c0"><a name="h.5v3pzda74g8r"></a><span>4</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.3ijw5h97ghxu"></a><span>Buffer</span></p></td><td class="c5"><p class="c0"><a name="h.bzlfbrbu9tnj"></a><span>2</span></p></td><td class="c5"><p class="c0"><a name="h.eo2dmvwtebsg"></a><span>2</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.ye9tnj8vo3cp"></a><span>Water</span></p></td><td class="c5"><p class="c0"><a name="h.x1keh7vo5pkv"></a><span>13</span></p></td><td class="c5"><p class="c0"><a name="h.ugqtocesucsy"></a><span>13</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.93ewrgz4fbjg"></a><span>P</span></p></td><td class="c5"><p class="c0"><a name="h.3vv55o7v7onu"></a><span>1</span></p></td><td class="c5"><p class="c0"><a name="h.mqmyn3aias9t"></a><span>1</span></p></td></tr><tr><td class="c5"><p class="c0"><a name="h.3jz94te8z8mv"></a><span>Total</span></p></td><td class="c5"><p class="c0"><a name="h.svrvg9osp1eh"></a><span>20</span></p></td><td class="c5"><p class="c0"><a name="h.ebqv9yeqx72u"></a><span>20</span></p></td></tr></tbody></table>