Team:Rutgers/Notebook

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<time class="cbp_tmtime" datetime="2013-06-01 18:30"><span>6/01/13</span> <span>8:00</span></time>
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<div class="cbp_tmicon cbp_tmicon-phone"></div>
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<h2>The Rutgers iGEM team sets out to find a lab.</h2>
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</div>
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</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-06-28 12:04"><span>6/28/13</span> <span>8:00</span></time>
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<div class="cbp_tmicon cbp_tmicon-screen"></div>
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<div class="cbp_tmlabel">
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<h2>Lab Found!</h2>
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<p>Dr. Sagar Khare in the Center for Integrative Proteomics Research offers us laboratory space and supplies to house the Rutgers iGEM team.</p>
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</div>
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</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-07-01 05:36"><span>7/01/13</span> <span>08:00</span></time>
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<div class="cbp_tmicon cbp_tmicon-mail"></div>
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<div class="cbp_tmlabel">
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<h2>First week of July</h2>
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<p>Lab safety training: Competent cell preparation, Plate preparation, Plate and cell negative control testing, Confirmation of plate antibiotics and negative controls , Acquisition of CRE recombinase protein from Dr. Pal Maliga at Waksman Institute of Microbiology.</p>
 +
<p>Transform the following: mRFP (I13521), GFP (I13522), CFP (I13600), mOrange (E2050), LuxR (C0062), LuxI (C0061), LuxBox (R0063), pSB1C3, CRE (in pET29 vector)</p>
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</div>
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</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-07-08 13:15"><span>7/08/13</span> <span>8:00</span></time>
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<div class="cbp_tmicon cbp_tmicon-phone"></div>
 +
<div class="cbp_tmlabel">
 +
<h2>Second week of July</h2>
 +
<p>Start overnights of transformations, Minipreps of genes, Digestions of LuxR and LuxBox, Gel Electrophoresis (failed digestion attempt), Start overnights of transformations, Minipreps of genes, Gel Electrophoresis, Ligation of LuxR and LuxBox, Transformation of L1 ( LuxR+LuxBox)</p>
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</div>
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</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-07-19 21:30"><span>7/19/13</span> <span>8:00</span></time>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
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<h2>July 15th-19th</h2>
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<p>Start overnight of L1, Miniprep L1, Review sequencing data, Failed ligation attempt, Start overnights of transformations, Minipreps of genes, Digestions of LuxR and LuxBox, Gel Electrophoresis (failed digestion attempt), Start overnights of transformations, Minipreps of genes, Gel Electrophoresis, Ligation of LuxR and LuxBox, Transformation of L1 ( LuxR+LuxBox)</p>
 +
</div>
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</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-17 12:11"><span>7/22/13</span> <span>8:00</span></time>
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<div class="cbp_tmicon cbp_tmicon-screen"></div>
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<div class="cbp_tmlabel">
 +
<h2>July 22nd-26th</h2>
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<p>PCR of mRFP loxp site addition, PCR of GFP loxp site addition, Gel electrophoresis confirmation, Digestion of LuxR and LuxBox</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-18 09:56"><span>7/29/13</span> <span>8:00</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-phone"></div>
 +
<div class="cbp_tmlabel">
 +
<h2>July's last days</h2>
 +
<p>Reconsider CRE recombinase project, Quorum sensing degradation design</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-18 09:56"><span>8/5/13</span> <span>8:00</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-phone"></div>
 +
<div class="cbp_tmlabel">
 +
<h2>August!</h2>
 +
<p>New plasmid design for LuxR, LuxI, qsdA degradation, gfp. Order from genscript</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-18 09:56"><span>9/2/13</span> <span>8:00</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-phone"></div>
 +
<div class="cbp_tmlabel">
 +
<h2>First week of September</h2>
 +
<p>Arrival of genscript plasmids, Transformation for BBa_K1206000, Miniprep BBa_K1206000, Sequence verification</p>
 +
</div>
 +
</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-04-18 09:56"><span>9/16/13</span> <span>8:00</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-phone"></div>
 +
<div class="cbp_tmlabel">
 +
<h2>Mid-July</h2>
 +
<p>Assay for fluorescence of GFP in project plasmid</p>
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</div>
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</li>
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</ul>
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</div>
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</div>
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//  Creating our button in JS for smaller screens
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace. 
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// http://tympanus.net/codrops/2013/05/08/responsive-retina-ready-menu/comment-page-2/#comment-438918
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!align="center"|[[Team:Rutgers|Home]]
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document.onclick = function(e) {
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!align="center"|[[Team:Rutgers/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Rutgers Official Team Profile]
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!align="center"|[[Team:Rutgers/Project|Project]]
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!align="center"|[[Team:Rutgers/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Rutgers/Modeling|Modeling]]
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!align="center"|[[Team:Rutgers/Notebook|Notebook]]
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!align="center"|[[Team:Rutgers/Safety|Safety]]
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!align="center"|[[Team:Rutgers/Attributions|Attributions]]
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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</script>
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</body>
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</html>

Revision as of 02:16, 28 September 2013

Rutgers notebook

  • The Rutgers iGEM team sets out to find a lab.

  • Lab Found!

    Dr. Sagar Khare in the Center for Integrative Proteomics Research offers us laboratory space and supplies to house the Rutgers iGEM team.

  • First week of July

    Lab safety training: Competent cell preparation, Plate preparation, Plate and cell negative control testing, Confirmation of plate antibiotics and negative controls , Acquisition of CRE recombinase protein from Dr. Pal Maliga at Waksman Institute of Microbiology.

    Transform the following: mRFP (I13521), GFP (I13522), CFP (I13600), mOrange (E2050), LuxR (C0062), LuxI (C0061), LuxBox (R0063), pSB1C3, CRE (in pET29 vector)

  • Second week of July

    Start overnights of transformations, Minipreps of genes, Digestions of LuxR and LuxBox, Gel Electrophoresis (failed digestion attempt), Start overnights of transformations, Minipreps of genes, Gel Electrophoresis, Ligation of LuxR and LuxBox, Transformation of L1 ( LuxR+LuxBox)

  • July 15th-19th

    Start overnight of L1, Miniprep L1, Review sequencing data, Failed ligation attempt, Start overnights of transformations, Minipreps of genes, Digestions of LuxR and LuxBox, Gel Electrophoresis (failed digestion attempt), Start overnights of transformations, Minipreps of genes, Gel Electrophoresis, Ligation of LuxR and LuxBox, Transformation of L1 ( LuxR+LuxBox)

  • July 22nd-26th

    PCR of mRFP loxp site addition, PCR of GFP loxp site addition, Gel electrophoresis confirmation, Digestion of LuxR and LuxBox

  • July's last days

    Reconsider CRE recombinase project, Quorum sensing degradation design

  • August!

    New plasmid design for LuxR, LuxI, qsdA degradation, gfp. Order from genscript

  • First week of September

    Arrival of genscript plasmids, Transformation for BBa_K1206000, Miniprep BBa_K1206000, Sequence verification

  • Mid-July

    Assay for fluorescence of GFP in project plasmid

Retrieved from "http://2013.igem.org/Team:Rutgers/Notebook"