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Team:NTU Taiwan/index.html
From 2013.igem.org
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<li> <a href="#Modeling">Modeling</a></li> | <li> <a href="#Modeling">Modeling</a></li> | ||
<li> <a href="#Protocol">Protocol</a> </li> | <li> <a href="#Protocol">Protocol</a> </li> | ||
| - | <li> <a href=" | + | <li> <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&group=NTU_Taiwan">Parts</a></li> |
<li> <a href="#ProjectResult">Result</a></li> | <li> <a href="#ProjectResult">Result</a></li> | ||
</ul> | </ul> | ||
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<div class="container divide essay"> | <div class="container divide essay"> | ||
<p> | <p> | ||
| - | Shuttle vector is a vector that can propagate in different species, used to make gene amplifications quickly in <i>E. coli</i>, mutagenesis, and PCR.[1] Generally, these plasmid vectors contain genetic material derived from the <i>E.coli</i>, origin of replication which enable them to be propagated in <i>E.coli</i> cells prior to transformation into yeast cells. | + | Shuttle vector is a vector that can propagate in different species, used to make gene amplifications quickly in <i>E. coli</i>, mutagenesis, and PCR.[1] Generally, these plasmid vectors contain genetic material derived from the <i>E.coli</i>, origin of replication which enable them to be propagated in <i>E.coli</i> cells prior to transformation into yeast cells, and a selectable marker (mainly the ß-lactamase gene, amp) for the bacterial host. |
</p><p> | </p><p> | ||
The most common shuttle vector is yeast shuttle which can be propagated in yeast and <i>E.coli</i>.There are four types of shuttle vectors.[2] | The most common shuttle vector is yeast shuttle which can be propagated in yeast and <i>E.coli</i>.There are four types of shuttle vectors.[2] | ||
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<div class="divide essay container"> | <div class="divide essay container"> | ||
<p><b>National Taiwan University</b></p> | <p><b>National Taiwan University</b></p> | ||
| + | <img src="http://www.bst.ntu.edu.tw/faculty/ChenYR/ChenYR1.files/image009.png" width=300> | ||
<p>Our supervisor Dr. Yen-rong Chen for making our adventure in iGEM possible.</p> | <p>Our supervisor Dr. Yen-rong Chen for making our adventure in iGEM possible.</p> | ||
| + | <img src="http://www.bst.ntu.edu.tw/faculty/HuangCT/HuangCT1.files/image010.png" width=300> | ||
| + | <p>Dr. Ching-Tsan Huang for providing us with the shuttle vectors pGAPZA plasmid.</p> | ||
| + | <img src="http://www.ym.edu.tw/bps/linjj.files/image001.jpg" width=300> | ||
<p>Dr. Jing-Jer Lin for providing us with the shuttle vectors, pRS424 and pRS423.</p> | <p>Dr. Jing-Jer Lin for providing us with the shuttle vectors, pRS424 and pRS423.</p> | ||
| + | <img src="http://www.bst.ntu.edu.tw/faculty/LinCH/LinCH1.files/image010.jpg" width=300> | ||
| + | <img src="http://www.ac.ntu.edu.tw/images/people/Kaiyin%20Lo.jpg" width=300> | ||
<p>Dr. Ching-Hsuan Lin, Dr. Kai-Yin Lo and Dr. Jing-Jer Lin for their advice with the yeast molecular techniques.</p> | <p>Dr. Ching-Hsuan Lin, Dr. Kai-Yin Lo and Dr. Jing-Jer Lin for their advice with the yeast molecular techniques.</p> | ||
| + | <img src="http://www.chem.sinica.edu.tw/faculty/photo/B/cherri.jpg" width=300> | ||
<p>Dr. Chao-Ping Hsu for her help with the modeling part of our project.</p> | <p>Dr. Chao-Ping Hsu for her help with the modeling part of our project.</p> | ||
| + | <img src="http://www.bst.ntu.edu.tw/faculty/LeeKT/LeeKT1.files/image010.png" width=300> | ||
<p>Dr. Kung-Ta Lee for his advice of how to measure the heat produce of YeasTherm.</p><br/> | <p>Dr. Kung-Ta Lee for his advice of how to measure the heat produce of YeasTherm.</p><br/> | ||
<p><b>Institute of Molecular Biology, Academia Sinica</b></p> | <p><b>Institute of Molecular Biology, Academia Sinica</b></p> | ||
| + | <img src="http://www.cctmf.org.tw/campcurie/2011/images/speakers/1-2.jpg" width=300> | ||
| + | <img src="http://www.imb.sinica.edu.tw/ch/adm-photo/jeng.jpg" width=200> | ||
<p>Lab N214 Dr. Michael M.C. Lai and Dr. King-Song Jeng for providing us with lab equipment and materials.</p><br/> | <p>Lab N214 Dr. Michael M.C. Lai and Dr. King-Song Jeng for providing us with lab equipment and materials.</p><br/> | ||
<p><b>Iwate University</b></p> | <p><b>Iwate University</b></p> | ||
| + | <img src="http://www.iwate-u.ac.jp/coe/en/images/kikuito.jpg" width=300> | ||
<p>Dr. Kikukatsu Ito for providing us the important gene, SrUCP DNA, for our project.<p><br/> | <p>Dr. Kikukatsu Ito for providing us the important gene, SrUCP DNA, for our project.<p><br/> | ||
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<div class="container" style="margin-top: 20px"> | <div class="container" style="margin-top: 20px"> | ||
<img class="pull-right img-responsive" src="https://static.igem.org/mediawiki/2013/1/1d/Pgapza.png" alt-src="./images/result/pgapza.png" width=550> | <img class="pull-right img-responsive" src="https://static.igem.org/mediawiki/2013/1/1d/Pgapza.png" alt-src="./images/result/pgapza.png" width=550> | ||
| - | <div class="col-md-4" style="margin-top: 140px"><p> This is the pGAPZa | + | <div class="col-md-4" style="margin-top: 140px"><p> This is the pGAPZa which had been digested by <i>Bgi</i>II.</p></div> |
</div> | </div> | ||
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</script> | </script> | ||
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Revision as of 02:37, 28 September 2013
