Kyoto:projectRNA/futureview

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==FutureView-construction and assay==
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==FutureView-assay==
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===Construction===
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===Assay===
===Assay===
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<1><p>inducible tetR aptamer to drive GFP-expression on plate. As negative controls, we will use RNA with antisense, attenuator, spinach, no-RNA and attenuator-tetR aptamer. As positive controls, we will also use GFP. As a consequence, we will expect the colony analyzing X-gal express the blue with &beta-gal, the negative control express the white, and that the positive control do the blue.</p>
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<p>2. </p>
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<p>3.qualitative assay of spinach assay</p>
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<p>We will check that DFHBI fluorescence on a plate with spinach. We cultivate IPTG-inducible Spinach in a liquid culture under a shading condition, and add DFHBI. Then we check whether this sample fluorescence after centrifugation. We also check spinach-GFP and antisense-spinach.As a result, we expect the pellet fluorescence just with spinach and DFHBI.</p>
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<p>3. </p>
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<p>4. </p>
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<p>5. </p>
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<iframe src='https://skydrive.live.com/embed?cid=35CDFBF147936D18&resid=35CDFBF147936D18%216990&authkey=ACQxfFDhXAszgko&em=2&wdAr=1.3333333333333333' width='350px' height='285px' frameborder='0'>これは、<a target='_blank' href='http://office.com/webapps'>Office Web Apps</a> の機能を利用した、<a target='_blank' href='http://office.com'>Microsoft Office</a> の埋め込み型のプレゼンテーションです。</iframe>
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Revision as of 03:35, 28 September 2013

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FutureView-assay

Assay

<1>

inducible tetR aptamer to drive GFP-expression on plate. As negative controls, we will use RNA with antisense, attenuator, spinach, no-RNA and attenuator-tetR aptamer. As positive controls, we will also use GFP. As a consequence, we will expect the colony analyzing X-gal express the blue with &beta-gal, the negative control express the white, and that the positive control do the blue.

2.

3.qualitative assay of spinach assay

We will check that DFHBI fluorescence on a plate with spinach. We cultivate IPTG-inducible Spinach in a liquid culture under a shading condition, and add DFHBI. Then we check whether this sample fluorescence after centrifugation. We also check spinach-GFP and antisense-spinach.As a result, we expect the pellet fluorescence just with spinach and DFHBI.

3.

4.

5.

6

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