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Kyoto:projectRNA/futureview
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==FutureView-assay== | ==FutureView-assay== | ||
===Assay=== | ===Assay=== | ||
| - | <1><p>inducible tetR aptamer to drive GFP-expression on plate. As negative controls, we will use RNA with antisense, attenuator, spinach, no-RNA and attenuator-tetR aptamer. As positive controls, we will also use GFP. As a consequence, we will expect the colony analyzing X-gal express the blue with &beta-gal, the negative control express the white, and that the positive control do the blue.</p> | + | <p>1. Qualitative assay of tetR</p> |
| + | <p>inducible tetR aptamer to drive GFP-expression on plate. As negative controls, we will use RNA with antisense, attenuator, spinach, no-RNA and attenuator-tetR aptamer. As positive controls, we will also use GFP. As a consequence, we will expect the colony analyzing X-gal express the blue with &beta-gal, the negative control express the white, and that the positive control do the blue.</p> | ||
<p>2. </p> | <p>2. </p> | ||
<p></p> | <p></p> | ||
| - | <p>3. | + | <p>3. Qualitative assay of spinach assay</p> |
<p>We will check that DFHBI fluorescence on a plate with spinach. We cultivate IPTG-inducible Spinach in a liquid culture under a shading condition, and add DFHBI. Then we check whether this sample fluorescence after centrifugation. We also check spinach-GFP and antisense-spinach.As a result, we expect the pellet fluorescence just with spinach and DFHBI.</p> | <p>We will check that DFHBI fluorescence on a plate with spinach. We cultivate IPTG-inducible Spinach in a liquid culture under a shading condition, and add DFHBI. Then we check whether this sample fluorescence after centrifugation. We also check spinach-GFP and antisense-spinach.As a result, we expect the pellet fluorescence just with spinach and DFHBI.</p> | ||
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| - | <iframe src='https://skydrive.live.com/embed?cid=35CDFBF147936D18&resid=35CDFBF147936D18%216990&authkey=ACQxfFDhXAszgko&em=2&wdAr=1.3333333333333333' width=' | + | <iframe src='https://skydrive.live.com/embed?cid=35CDFBF147936D18&resid=35CDFBF147936D18%216990&authkey=ACQxfFDhXAszgko&em=2&wdAr=1.3333333333333333' width='700px' height='570px' frameborder='0'>これは、<a target='_blank' href='http://office.com/webapps'>Office Web Apps</a> の機能を利用した、<a target='_blank' href='http://office.com'>Microsoft Office</a> の埋め込み型のプレゼンテーションです。</iframe> |
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{{Template:Kyoto/footer}} | {{Template:Kyoto/footer}} | ||
Revision as of 03:37, 28 September 2013
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FutureView-assay
Assay
1. Qualitative assay of tetR
inducible tetR aptamer to drive GFP-expression on plate. As negative controls, we will use RNA with antisense, attenuator, spinach, no-RNA and attenuator-tetR aptamer. As positive controls, we will also use GFP. As a consequence, we will expect the colony analyzing X-gal express the blue with &beta-gal, the negative control express the white, and that the positive control do the blue.
2.
3. Qualitative assay of spinach assay
We will check that DFHBI fluorescence on a plate with spinach. We cultivate IPTG-inducible Spinach in a liquid culture under a shading condition, and add DFHBI. Then we check whether this sample fluorescence after centrifugation. We also check spinach-GFP and antisense-spinach.As a result, we expect the pellet fluorescence just with spinach and DFHBI.
3.
4.
5.
6
