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Team:WHU-China/templates/acknowledgement
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<h1 font-size=20px ><a name="attribution"></a><strong>Attribution</strong></h1> | <h1 font-size=20px ><a name="attribution"></a><strong>Attribution</strong></h1> | ||
| + | Based on the published researches about CRISPR system and tandem promoters, we are trying to explore multi-stage regulation through the combination of them.</br></br> | ||
| + | We got the plasmid containing dCas9 gene from Doudna Lab freely of Addgene.</br></br> | ||
We successfully express the dead Cas9(dCas9) BBa_k1081000 in standard backbone pSB1C3 and pSB1A2 in E.coli strain k12 and fused the dCas9 with omega subunit of RNA polymease.</br></br> | We successfully express the dead Cas9(dCas9) BBa_k1081000 in standard backbone pSB1C3 and pSB1A2 in E.coli strain k12 and fused the dCas9 with omega subunit of RNA polymease.</br></br> | ||
| + | Then we donated functional dCas9 Bio-Brick (BBa_k1081000) to BGI (Beijing genomics institution) 2013 iGEM team.</br></br> | ||
We constructed seven tandem double promoters BBa_k1081002, BBa_k1081003, BBa_k1081004, BBa_k1081005, BBa_k1081006, BBa_k1081007 and BBa_k1081008. </br></br> | We constructed seven tandem double promoters BBa_k1081002, BBa_k1081003, BBa_k1081004, BBa_k1081005, BBa_k1081006, BBa_k1081007 and BBa_k1081008. </br></br> | ||
| - | + | We constructed 7 guide RNA coding sequences and got them sequencced right.</br></br> | |
We proved the feasibility of multiple modulation. </br></br> | We proved the feasibility of multiple modulation. </br></br> | ||
</p> | </p> | ||
Revision as of 03:37, 28 September 2013
Acknowledgement
Participating in iGEM is a comprehensive work, which can not be done without the indispensible help from our friends and guiders. Hereby we want to sincerely thanks:- Kenji Adzuma, for his patient guiding of the enzymatic modeling.
- Yancheng Liu, for his marvelous programming and helps in processing data.
- Prof. David Liu and Vikram Pattanayak, for their generous providing of data for our Cas9 model.
- Kuanwei Sheng, Jiawei Hang, Wenxiong Zhou, Boxiang Wang, for their devotion in passing their experience and ideas about iGEM to us.
- Prof. Fenyong Liu and Xiangdong Chen, for their providing of special experiment facilities and resouce.
- Yao Yang, for his helpful assistance in revising the model. Zhongqiao Lin and Qiaolin He, for their timely help in art designing parts.
- Tengfei Ma, Shimeng Liu, Yun Huang, Liangliang Ji, Wenjia Gu, for their contributions to the brainstorm.
- Prof. Zhixiong Xie, Prof. Xiangdong Gao, Prof.Yu Chen and College of Life Science, WHU, for their continuous support.
