Template:Kyoto/Notebook/Aug 15

From 2013.igem.org

(Difference between revisions)
(LB Medium Plate)
(Miniprep)
 
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!DNA||concentration[µg/mL]||260/280||260/230  
!DNA||concentration[µg/mL]||260/280||260/230  
|-
|-
-
|BBa_B0034(1)||84.1 ||2.11 ||2.01
+
|BBa_B0034(1)||84.1||2.11||2.01
|-
|-
-
|BBa_B0034(2)||86.9 ||2.03 ||2.02
+
|BBa_B0034(2)||86.9||2.03||2.02
|-
|-
-
|BBa_R0011(1)||78.3 ||2.26 ||2.02
+
|BBa_R0011(1)||78.3||2.26||2.02
|-
|-
-
|BBa_R0011(2)||88.9 ||2.24 ||2.02
+
|BBa_R0011(2)||88.9||2.24||2.02
|-
|-
-
|BBa_P0412(1)||15.61 ||2.10 ||1.93
+
|BBa_P0412(1)||15.61||2.10||1.93
|-
|-
-
|BBa_P0412(2)||12.93 ||2.18 ||1.91
+
|BBa_P0412(2)||12.93||2.18||1.91
|}
|}
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Latest revision as of 03:42, 28 September 2013

Contents

Aug 15

Liquid Culture

Nakamoto

Samplemedium
8/10 BBa_B0034(1)LB (+Amp)
8/10 BBa_B0034(2)LB (+Amp)
8/10 BBa_R0011(1)LB (+CP)
8/10 BBa_R0011(2)LB (+CP)
8/10 BBa_P0412(1)LB (+CP)
8/10 BBa_P0412(2)LB (+CP)

Transformation

Nakamoto

NameSampleCompetent CellsTotalPlate
BBa_I0500(Pbad/araC)21719LB (+Kan)
BBa_F1610(RBS-luxI-DT)21719LB (+Amp)
BBa_R0061(Plux)21719LB (+CP)
BBa_I13504(RBS-GFP-DT)21719LB (+CP)
BBa_I0462(RBS-luxR-DT)21719LB (+Amp)
BBa_K117000(lysis1)21719LB (+CP)
BBa_K112806(lysis2)21719LB (+CP)
BBa_K748002(lysis3)21719LB (+CP)
BBa_B0015(DT)21719LB (+CP)
BBa_Q04400(RBS-tetR-DT-Ptet)21719LB (+Kan)
BBa_I732020(RBS-lacZα-DT)21719LB (+CP)
BBa_J2310021719LB (+Amp)

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
BBa_B0034(1)84.12.112.01
BBa_B0034(2)86.92.032.02
BBa_R0011(1)78.32.262.02
BBa_R0011(2)88.92.242.02
BBa_P0412(1)15.612.101.93
BBa_P0412(2)12.932.181.91