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Future Directions
Alternative to ChIP.
- MaGellin can also be used to screen the binding specificity of transcription factors (TF) and other DNA binding domains, with methylation serving as an effective reporter for targeted binding.
- By fusing a TF to the methyltransferase, its binding with the plasmid becomes easily detected by the associated methylation
- In this situation, one would vary the “target site” to see which DNA sequences have the strongest binding to the TF
- This technique would be significantly faster, simpler, and cheaper than a ChIP-based method
