"
Team:DTU-Denmark/Experiments
From 2013.igem.org
| Line 1: | Line 1: | ||
{{:Team:DTU-Denmark/Templates/StartPage|Experiments}} | {{:Team:DTU-Denmark/Templates/StartPage|Experiments}} | ||
==Toxicity of Intermediates== | ==Toxicity of Intermediates== | ||
| - | + | Determine whether the ions nitrite, nitrate and ammonium are toxic to ''E. coli'', and at what concentration. This experiment will use the Biolector to grow an ''E. coli'' strain that constitutively expresses RFP in the presence of these ions (at a range of concentrations) to determine the OD and fluorescence of the cells as a proxy for growth. | |
| - | + | ||
[[Team:DTU-Denmark/ToxicityExperiment|Toxicity Experiment]] | [[Team:DTU-Denmark/ToxicityExperiment|Toxicity Experiment]] | ||
| Line 8: | Line 7: | ||
== Verify nitrite stability in anaerobic, untransformed ''E. coli'' == | == Verify nitrite stability in anaerobic, untransformed ''E. coli'' == | ||
| - | + | Grow ''E. coli'' anaerobically in the presence of varying concentrations of nitrite; measure the amount of ammonia and nitrite present. | |
[[Team:DTU-Denmark/Experiment1a|Experiment 1a]] | [[Team:DTU-Denmark/Experiment1a|Experiment 1a]] | ||
| Line 14: | Line 13: | ||
== Measure production of N<sub>2</sub>O from Nitrite NO<sub>2</sub><sup>-</sup> anaerobically == | == Measure production of N<sub>2</sub>O from Nitrite NO<sub>2</sub><sup>-</sup> anaerobically == | ||
| - | + | Grow ''E. coli'' and ''P.aeroginosa'' anaerobically in the presence of varying concentrations of nitrite; measure the amount of nitrous oxide produced. | |
[[Team:DTU-Denmark/Experiment2|Experiment 2]] | [[Team:DTU-Denmark/Experiment2|Experiment 2]] | ||
| - | == Growth of Nir in Bioreactor == | + | == Measure consumption of Ammonium in Mutant 1 transformants compared to native ''E. coli''. |
| + | |||
| + | Grow AMO and HAO transformants aerobically in the presence of ammonia; measure concentration of ammonia. | ||
| + | |||
| + | [[Team:DTU-Denmark/Experiment4|Experiment 4]] | ||
| + | |||
| + | == Growth of Nir transformant in Bioreactor == | ||
[[Team:DTU-Denmark/Bioreactor|Bioreactor]] | [[Team:DTU-Denmark/Bioreactor|Bioreactor]] | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 15:25, 28 September 2013
Experiments
Contents |
Toxicity of Intermediates
Determine whether the ions nitrite, nitrate and ammonium are toxic to E. coli, and at what concentration. This experiment will use the Biolector to grow an E. coli strain that constitutively expresses RFP in the presence of these ions (at a range of concentrations) to determine the OD and fluorescence of the cells as a proxy for growth.
Verify nitrite stability in anaerobic, untransformed E. coli
Grow E. coli anaerobically in the presence of varying concentrations of nitrite; measure the amount of ammonia and nitrite present.
Measure production of N2O from Nitrite NO2- anaerobically
Grow E. coli and P.aeroginosa anaerobically in the presence of varying concentrations of nitrite; measure the amount of nitrous oxide produced.
== Measure consumption of Ammonium in Mutant 1 transformants compared to native E. coli.
Grow AMO and HAO transformants aerobically in the presence of ammonia; measure concentration of ammonia.
Growth of Nir transformant in Bioreactor
