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Team:British Columbia/Notebook
From 2013.igem.org
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!align="center"|[[Team:British_Columbia/Attributions|Attributions]] | !align="center"|[[Team:British_Columbia/Attributions|Attributions]] | ||
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| + | ==Team CRISPR== | ||
| + | List of BioBricks that are/will be on the way: (CAM PLEASE CONFIRM) | ||
| + | |||
| + | Cas9: | ||
| + | 1) Cas9 - Joe and Anna | ||
| + | 2) pTet - | ||
| + | 3) const. promoter + Cas9 - Joe | ||
| + | 4) promoter + Cas9 + Leader + R + spacer + R (on Kan) | ||
| + | 5) L + R + spacer + R | ||
| + | |||
| + | Target: | ||
| + | 1) tracerRNA | ||
| + | 2) repeat + spacer + repeat - Vanins*2 + underlings | ||
| + | 3) leader - Ray, Fisal, Dan | ||
| + | |||
| + | Decoy: | ||
| + | 1) PAM + spacer (on Amp) | ||
| + | |||
==Team Caffeine== | ==Team Caffeine== | ||
Revision as of 01:18, 4 July 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Team CRISPR
List of BioBricks that are/will be on the way: (CAM PLEASE CONFIRM)
Cas9: 1) Cas9 - Joe and Anna 2) pTet - 3) const. promoter + Cas9 - Joe 4) promoter + Cas9 + Leader + R + spacer + R (on Kan) 5) L + R + spacer + R
Target: 1) tracerRNA 2) repeat + spacer + repeat - Vanins*2 + underlings 3) leader - Ray, Fisal, Dan
Decoy: 1) PAM + spacer (on Amp)
Team Caffeine
June 20
Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.
We're making a start - what are the other groups up to?
--grace.yi
