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Team:DTU-Denmark/pBAD SPL
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== pBAD synthetic promoter library == | == pBAD synthetic promoter library == | ||
| - | As a tool for expressing lethal proteins in ''E.coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL link]. | + | As a tool for expressing lethal proteins in ''E.coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL (link)]. |
| + | == Method == | ||
| + | === USER cloning for SPL === | ||
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| + | == Results == | ||
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| + | == Example of use == | ||
[[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]] | [[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]] | ||
[[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]] | [[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]] | ||
[[File:Graf.PNG|thumbnail|upright=2|left|alt=Alt text|Caption text]] | [[File:Graf.PNG|thumbnail|upright=2|left|alt=Alt text|Caption text]] | ||
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| + | See also [[HelloWorld]]. | ||
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Revision as of 19:00, 28 September 2013
pBAD SPL
Contents |
pBAD synthetic promoter library
As a tool for expressing lethal proteins in E.coli we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 (link).
Method
USER cloning for SPL
Results
Example of use
See also HelloWorld.
