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Team:Paris Saclay/Notebook/June/26
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Revision as of 20:53, 28 September 2013
Notebook : June 26
Lab work
- Design of the oligonucleotide for the amplification of Pbphr1 (intergenic sequence upstream) for Pseudomonas pseudoalcaligenes PbphD and KF707 (intergenic sequence upstream) for Burkholderia xenovorans LB400
- 500 ml Buffers (TB: the Transformation Buffer) prepared to make super competent bacteria, stored at 4°C
- 250 ml of culture of E.Coli DH5αZ1 in LB prepared for overnight culture, with an initial Do (Optical Density) of 0.04 at 17h30min
- Protocol of extraction of the genomic DNA from bacteria (E.Coli DH5α) written
- Documentation about the fnr activator (Pfnr+) in iGEM registry
- Team meeting and discussion with Munich team (Skype)
