Template:Team:Uppsala/JS/notebook

From 2013.igem.org

(Difference between revisions)
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   '<h2>Other experiments</h2><br>-<br></p></div>';  
   '<h2>Other experiments</h2><br>-<br></p></div>';  
 +
}
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  else if(id == 'd201382')
 +
{
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ds = '<div id="dairy-text"><h1>Friday 2013-08-02</h1><br><b>Name of participants: </b>Anton B, Christoffer A, Mikael S, Jens I, Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E, Magnus, Pontus, Sabri, Nils<br><br><h2>Ongoing constructs:</h2><br><b>E.coli:</b><br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>108. pSBA15-J23106-B0034-IspA<br>109. pSBA15-K206000-B0034-IspA<br>110. pSBA15-K206001-B0034-IspA<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>140. pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141. pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142. pSB3T5-CP1-B0034-UGTCs2-6xHis<br>163. pEL3S17-ZCD-His-B0034-UGTCs2-His<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>177. pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178. pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183. pEL3A15-B0034-CrtZ<br>184. pSB1C3-B0034-CrtZ<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>191. pSB1A2-fusionGFP<br>193. pSB1C3-amilGFP<br>194. pSB1C3-B0034-amilCP<br>195. pSB1C3-cjBlue<br>196. pSB1C3-eforRed<br>197. pSB1C3-aeBlue<br>198. pSB1C3-B0034-amilGFP<br>199. pSb1C3-amajCFP<br>200. pSB1C3-B0034-asCP<br>202. pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>204. pEL3S17-J23110-HIS-STS<br>205. pSBLB4C15-CP1-B0034-HIS-TAL<br>206. pSB1C3-J23101-B0034-TAL<br>207. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br><br><b>L. reuteri:</b><br>3. DSM 20016 pVS2<br>4. 100-23 no plasmid<br>12. DSM 20016 no plasmid<br>23. 100-23 pSBLb4C15-red<br>24. DSM 20016 pSBLb4C15-red<br>26. 100-23 pSBLbEcC2-red<br>27. DSM 20016 pSBLbEcC2-red<br>28. 100-23 pVS2<br><br><h2>Todays work</h2><br><b>Plasmid preparation: </b><br>139.1.1 pSB3K3-J23101-B0032-BFP <br>139.1.2 pSB3K3-J23101-B0032-BFP<br>191.1 pSB1A2-fusionGFP<br>191.2 pSB1A2-fusionGFP<br><br><b>Ligation:</b><br>4.pSB1A3-Red, 147.pSB1C3-B0034-Thioredoxin, 125.pSB1C3-ZCD-His-><br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br><br><b>Streaking of plasmid-free cells from frozen stock as control of C-resistance: </b><br>Lb4.1 reuteri. 100-23 no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak of construct:</b><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br><b>Screening:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Overnight:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Gel electrophoresis:</b><br>PCR of 152. pUC57-B0034-Exp-Linker-Miraculin-Linker-His to extract Miraculin with biobrick prefix and suffix<br><br><h2>Results</h2><br><b>Today:</b><br><b>Plasmid preparation:</b> <br>139.1.1 pSB3K3-J23101-B0032-BFP: 51.9 ng/µl <br>139.1.2 pSB3K3-J23101-B0032-BFP: 53.6 ng/µl<br>191.1 pSB1A2-fusionGFP: 78.9 ng/µl<br>191.2 pSB1A2-fusionGFP: 74.1 ng/µl</div>'
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}
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    else if(id == 'd201383')
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{
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ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>'
  }
  }
  else if(id == 'd2013919')
  else if(id == 'd2013919')

Revision as of 02:21, 29 September 2013

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