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Template:Team:Uppsala/JS/notebook
From 2013.igem.org
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ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>' | ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>' | ||
| + | } | ||
| + | else if(id == 'd201383') | ||
| + | { | ||
| + | ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>' | ||
| + | } | ||
| + | else if(id == 'd201384') | ||
| + | { | ||
| + | ds = '<div id="dairy-text"><h1>Sunday 2013-08-04</h1><br><b>Name of participants: </b>Emil M, Magnus, Pontus<br><br><h2>Ongoing constructs:</h2><br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br><br><h2>Todays work:</h2><br><b>Overnight:</b><br>10. D5α<br><br><h2>Characterization</h2><br><b>Detection of TAL activity through spectroscopy (day 1)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2Other experiments:</b><br><b>Competent cell test</b><br><br></div>' | ||
| + | } | ||
| + | else if(id == 'd201385') | ||
| + | { | ||
| + | ds = '<div id="dairy-text"><h1>Monday 2013-08-05</h1><br><b>Name of participants: </b>Anton B, Viktor T, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri:</b><br>23. 100-23 pSBLb4C15-red<br>25. 100-23 pSBLbC2-red<br>28. 100-23 pVS2<br><br><b>E. coli:</b><br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Todays work</h2><br><b>Competent cells</b><br>Lb4.1 reuteri 100-23 no plasmid<br><br><b>Lambda red transformation:</b><br>Crt<br><br><b>Transformation:</b><br>reuteri 100-23 pSB1C3-red (1 tube, to see if it would work in Lb, died in electroporation)<br>Lb23. reuteri 100-23 pSBLb4C15-red (5 tubes, 1 died in electroporation)<br>Lb25. reuteri 100-23 pSBLbC2-red (1 tube)<br>Lb28. reuteri 100-23 pVS2 (2 tubes, 1 died in electroporation)<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Screening:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br><br><h2>Characterization</h2><br><b>Detection of TAL activity through spectroscopy (day 2)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2>Other experiments<h2><br><b>Casting LB-agar plates: </b><br>Kanamycin, 63 plates<br>Chloramphenicol, 61 plates<br>Spectinomycin, 65 plates<br>Tetracycline, 32 plates<br>Ampicillin, 30 plates<br><br><b>Competent cells (D5α)</b></div>' | ||
| + | } | ||
| + | else if(id == 'd201386') | ||
| + | { | ||
| + | ds = '<div id="dairy-text"><h1>Tuesday 2013-08-06</h1><br><b>Name of participants:</b> Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Anton B, Mikael S, Viktor T<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>57.3.pSB1C3-B0034-Idi<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>6. pSB1K3-red<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>111. pSBLb4C15-red<br>139. pSB3K3-J23101-B0032-BFP<br>165. pSB1C3-RBS-tsPurle<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>194. pSB1C3-B0034-amilCP<br>198. pSB1C3-B0034-amilGFP<br><br><b>L. reuteri:</b><br>23. 100-23 pSBLb4C15-red<br>25. 100-23 pSBLbC2-red<br>28. 100-23 pVS2 <br><br><h2>Todays work:</h2><br><b>Transformation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Restreak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>115. pSB3K5-J23101-B0034-His-TAL<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Digest:</b><br>6. pSB1K3-red<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>68.2 pSB3K3-CP1-B0032-BFP (EcoRI + PstI)<br>69.4 pSB3K3-CP8-B0032-BFP (EcoRI + PstI)<br>70.3 pSB3K3-CP11-B0032-BFP (EcoRI + PstI)<br>71.1 pSB3K3-CP29-B0032-BFP (EcoRI + PstI)<br>72.2 pSB3K3-CP30-B0032-BFP (EcoRI + PstI)<br>73.4 pSB3K3-CP41-B0032-BFP (EcoRI + PstI)<br>74. pSB3K3-CP44-B0032-BFP (EcoRI + PstI)<br>111. pSBLb4C15-red (XbaI + PstI)<br>165. pSB1C3-RBS-tsPurle (XbaI + PstI)<br>166. pSB1C3-RBS-scOrange (XbaI + PstI)<br>167. pSB1C3-RBS-fwYellow (XbaI + PstI)<br>194. pSB1C3-B0034-amilCP (XbaI + PstI)<br>198. pSB1C3-B0034-amilGFP (XbaI + PstI)<br><br><b>Ligation:</b><br>B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin<br>B0034-Thioredoxin, 1. pSB1C3-red (X, P) -> 147. pSB1C3-B0034-Thioredoxin<br><br><b>Gel: </b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br><br><b>Screening:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br>204.(1-4) pEL3S17-J23110-B0034-HIS-STS<br>171(1-4) pEL3S17-J23110-B0034-4CL-B0034-STS<br><br><b>Mutagenesis:</b><br>43.7 pSB1C3-B0034-TAL<br>57.3.pSB1C3-B0034-Idi -> 129.pSB1C3-Idi(mut)<br><br><v>Sequencing preparation:</b><br>139.1 pSB3K3-J23101-B0032-BFP<br>185.9 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>185.10 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>O/N:</b><br>204.2 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br>111.7 pSBLb4C15-red<br><br><h2>Results:</h2><br><b>Gel:</b> <br>164.E.Coli Nissle-CrtY (clones from transformation plate) failed<br>201.E.Coli Nissle-CrtY-CrtI failed<br><br><b>Previous day:</b><br><b>Transformation: </b><br>No visual growth. Keeping them on growth for one more day.<br><br><h2>Characterization:</h2><br><b>Detection of TAL activity through spectroscopy (day 3)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2>Other Experiments:</h2><br>10.D5Alpha competence test<br><br><b>Preparation of buffers for Western Blot</b><br><br><b>Casting LB-agar plates:</b><br>Kanamycin, 33 plates<br>Spectinomycin, 31 plates<br><br></div>' | ||
} | } | ||
else if(id == 'd2013919') | else if(id == 'd2013919') | ||
Revision as of 06:44, 29 September 2013
