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Team:UNITN-Trento/Notebook/Labposts/08/46
From 2013.igem.org
(Difference between revisions)
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"title" : "<html>Target acquired, update in progress, that the mutagenesis begin</html>", | "title" : "<html>Target acquired, update in progress, that the mutagenesis begin</html>", | ||
"content" : "<html>I used the specific primer degnigned and created to insert RBS in the K952003 part before the amilGFP protein. For doing that I phosphorylated the primer with PNK chinase. In addition I doing 2 PCR to mutagenize the sequence using 100pg and 1ng of DNA template. The condition how the PCR works are: 67 degree of anealing, 1.30 mins of extention time and 1.5 ul of DMSO. It took the life the part K1065302</html>", | "content" : "<html>I used the specific primer degnigned and created to insert RBS in the K952003 part before the amilGFP protein. For doing that I phosphorylated the primer with PNK chinase. In addition I doing 2 PCR to mutagenize the sequence using 100pg and 1ng of DNA template. The condition how the PCR works are: 67 degree of anealing, 1.30 mins of extention time and 1.5 ul of DMSO. It took the life the part K1065302</html>", | ||
| - | "tags" : "Blue light | + | "tags" : "Blue light-mutagenesis" |
} | } | ||
Revision as of 08:43, 29 September 2013
{ "date" : "2013-08-19", "author" : "bruno", "title" : "Target acquired, update in progress, that the mutagenesis begin", "content" : "I used the specific primer degnigned and created to insert RBS in the K952003 part before the amilGFP protein. For doing that I phosphorylated the primer with PNK chinase. In addition I doing 2 PCR to mutagenize the sequence using 100pg and 1ng of DNA template. The condition how the PCR works are: 67 degree of anealing, 1.30 mins of extention time and 1.5 ul of DMSO. It took the life the part K1065302", "tags" : "Blue light-mutagenesis" }
