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From 2013.igem.org

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Revision as of 10:38, 29 September 2013

MiniPrep

We Miniprepped the following parts:

• Promoter and RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002])

• Magenta pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012])

• Yellow pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592010 BBa_K592010])

• Yellow pigment with RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_S05058 BBa_S05058])

• Terminator ([http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015])

• RFP control ([http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450 BBa_KJ04450])

NanoDrop results

Part	ng/ul
RFP control	80.7
Yellow w/ RBS	60.8
Promoter and RBS	127.8
Magenta pigment	46.9
Terminator	89.9
Yellow pigment	59.0
KAN plasmid	23.6

Digestion

Part	ng/ul from NanoDrop	ul need to have 250ng DNA	16ul - required ul DNA (amount of water needed for digestion)
RFP control	80.7	3.10	12.9
Yellow w/ RBS	60.8	4.11	11.89
Promoter and RBS	127.8	2.0	14.0
Magenta pigment	46.9	5.33	10.67
Terminator	89.9	2.80	13.2
Yellow pigment	59.0	4.24	11.76
KAN plasmid	23.6	10.60	5.4