"

From 2013.igem.org

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Revision as of 12:03, 29 September 2013

{ "date":"2013-07-02", "author":"viola", "title":"EFE+B0015", "content":"today i digested B015 in pSB1C3 with the enzymes E and X and EFE in pUC57 with E and S. I started a short cloning protocol with this ligation quantities:

LIGATION	CTRL	1:1	1:2	1:4
BUFFER T4	2 µl	2 µl	2 µl	2 µl
PLASMID (B0015)	2.5 µl	2.5 µl	2.5 µl	2.5 µl
INSEERT (EFE)	0 µl	1.28 µl	2.56 µl	5.12 µl
LIGASE T4	1 µl	1 µl	1 µl	1 µl
H2O	14.5 µl	13.2 µl	11.9 µl	10.6 µl

", "tags":"EFE" }