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Team:DTU-Denmark/HelloWorld
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[[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]] | [[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]] | ||
[[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]] | [[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]] | ||
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Here is a detailed guide on how to direct GFP to the periplasm ([https://2013.igem.org/Team:DTU-Denmark/Methods/Visualizing_GFP_in_the_periplasm link]). | Here is a detailed guide on how to direct GFP to the periplasm ([https://2013.igem.org/Team:DTU-Denmark/Methods/Visualizing_GFP_in_the_periplasm link]). | ||
Revision as of 13:21, 29 September 2013
Hello World Pilot Project
Contents |
Introduction
‘Hello World!’ are the first words a programmer prints when learning a new programming language. In analogy to this our team decided to do a ‘Hello World’ project in order to familiarize ourselves with lab techniques that we used later on to construct plasmids. Specifically we were performing PCR with uracil-containing primers, purifying PCR products and ligating them by means of USER cloning.
Since we are working with many periplasmic proteins, we wanted to try to target proteins to the periplasm. To do this, we used periplasmic signal peptides from the TAT and Sec pathways, and with a translational fusion of the signal peptide to GFP, we expressed GFP in the periplasm. Simultaneously, we expressed RFP in the cytoplasm.
Results
Here is a detailed guide on how to direct GFP to the periplasm (link).
Conclusions
Biobrick BBa_K1067009 successfully directs proteins to the periplasm in E. coli.
