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Team:UNITN-Trento/Notebook/Labposts/07/78
From 2013.igem.org
(Difference between revisions)
(Created page with "{ "date":"2013-07-06", "author":"viola", "title":"<html>cloning of EFE in pSPAC and pXyl vectors</html>", "content":"<html> Today i start the cloning to insert the ethylene formi...") |
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"author":"viola", | "author":"viola", | ||
"title":"<html>cloning of EFE in pSPAC and pXyl vectors</html>", | "title":"<html>cloning of EFE in pSPAC and pXyl vectors</html>", | ||
| - | "content":"<html> Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1| <html> <center> <table> <tr> <th>LIGATION</th> <th>EFE+B0015 in pSPAC</th> <th>EFE+B0015 in pXYL</th> </tr> <tr> <th>PLASMID CONC.</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>PLASMID LENGHT</th> <td>7082 BP</td> <td>3594 BP</td> </tr> <tr> <th>INSERT CONCENTRATION</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>INSERT LENGHT</th> <td>1251 BP</td> <td>1251 BP</td> </tr> <tr> <th>PLASMID USED</th> <td>50 ng/µl</td> <td>50 ng/µl</td> </tr> <tr> <th>REACTION VOLUME</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>BUFFER</th> <td>10 µl</td> <td>10 µl</td> </tr> </table> </center> </html>}} <html> </html> | + | "content":"<html> Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1| <html> <center> <table> <tr> <th>LIGATION</th> <th>EFE+B0015 in pSPAC</th> <th>EFE+B0015 in pXYL</th> </tr> <tr> <th>PLASMID CONC.</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>PLASMID LENGHT</th> <td>7082 BP</td> <td>3594 BP</td> </tr> <tr> <th>INSERT CONCENTRATION</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>INSERT LENGHT</th> <td>1251 BP</td> <td>1251 BP</td> </tr> <tr> <th>PLASMID USED</th> <td>50 ng/µl</td> <td>50 ng/µl</td> </tr> <tr> <th>REACTION VOLUME</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>BUFFER</th> <td>10 µl</td> <td>10 µl</td> </tr> </table> </center> </html>}} <html> </html>}} {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE PSPAC| <html> <center> <table> <tr> <th><font color=\"red\">EFE IN PSPAC</font></th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>INSERT (µl)</th> <td>0</td> <td>0.44</td> <td>0.88</td> <td>1.76</td> </tr> <tr> <th>WATER (µl)</th> <td>14.5 </td> <td>14.06</td> <td>13.62</td> <td>12.74</td> </tr> <tr> <th>LIGASE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <th>BUFFER (µl)</th> <td>2</td> <td>2</td> <td>2</td> <td>2</td> </tr> </table> </center> </html>}} {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE PXYL| <html> <center> <table> <tr> <th><font color=\"red\">EFE IN PXYL</font></th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>INSERT (µl)</th> <td>0</td> <td>0.87</td> <td>1.74</td> <td>3.48</td> </tr> <tr> <th>WATER (µl)</th> <td>14.5 </td> <td>13.63</td> <td>12.76</td> <td>11.02</td> </tr> <tr> <th>LIGASE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <th>BUFFER (µl)</th> <td>2</td> <td>2</td> <td>2</td> <td>2</td> </tr> </table> </center> </html>}} <html> </html>}} <html> </html>}} </html>", |
"tags":"BACILLUS" | "tags":"BACILLUS" | ||
} | } | ||
Revision as of 13:47, 29 September 2013
{ "date":"2013-07-06", "author":"viola", "title":"cloning of EFE in pSPAC and pXyl vectors", "content":" Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1|
| LIGATION | EFE+B0015 in pSPAC | EFE+B0015 in pXYL |
|---|---|---|
| PLASMID CONC. | 20 µl | 20 µl |
| PLASMID LENGHT | 7082 BP | 3594 BP |
| INSERT CONCENTRATION | 20 µl | 20 µl |
| INSERT LENGHT | 1251 BP | 1251 BP |
| PLASMID USED | 50 ng/µl | 50 ng/µl |
| REACTION VOLUME | 20 µl | 20 µl |
| BUFFER | 10 µl | 10 µl |
LIGATION TABLE PSPAC
| EFE IN PSPAC | CTRL | 1:1 | 1:2 | 1:4 |
|---|---|---|---|---|
| INSERT (µl) | 0 | 0.44 | 0.88 | 1.76 |
| WATER (µl) | 14.5 | 14.06 | 13.62 | 12.74 |
| LIGASE (µl) | 1 | 1 | 1 | 1 |
| PLASMID (µl) | 2.5 | 2.5 | 2.5 | 2.5 |
| BUFFER (µl) | 2 | 2 | 2 | 2 |
LIGATION TABLE PXYL
}} }} </html>",
"tags":"BACILLUS"
}
| EFE IN PXYL | CTRL | 1:1 | 1:2 | 1:4 |
|---|---|---|---|---|
| INSERT (µl) | 0 | 0.87 | 1.74 | 3.48 |
| WATER (µl) | 14.5 | 13.63 | 12.76 | 11.02 |
| LIGASE (µl) | 1 | 1 | 1 | 1 |
| PLASMID (µl) | 2.5 | 2.5 | 2.5 | 2.5 |
| BUFFER (µl) | 2 | 2 | 2 | 2 |
