Team:Bielefeld-Germany/Biosafety/Biosafety Strain

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<h2>Biosafety-Strain</h2>
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<h1>Safety Strain</h1>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Abstract">Biosafety Overview</a></div>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosafety Strain#Theory">Theory</a></div>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosafety Strain#Genetic_Approach">Genetic Approach</a></div>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosafety Strain#Results">Results</a></div>
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==Overview==
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[[Image:IGEM Bielefeld 2913 Biosafety E.coli bewaffnet save ohne hintergrund.jpg|250px|left|thumb]]
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We produced a auxotrophic safety strain which was the basic for our biosafety system. The strain consists Δdadx and Δalr knockouts which prevents the expression of alanine racemase. Alanine racemase is an isomerase that catalyzes the chemical reaction from L-alanine to D-alanine.<br><br>
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We produced a auxotrophic safety strain which was the basic for our biosafety system. The strain consists Δdadx and Δalr knockouts which prevents the expression of alanine racemase. Alanine racemase is an isomerase that catalyzes the chemical reaction from L-alanine to D-alanine.
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D-alanine appears in peptidoglycan which is an important component of the bacterial cell wall. So the safety strain depends on the supplementation of alanine racemase on the plasmid which causes a good plasmid stability. If the bacteria cast the plasmid it will die because of the fact that L-alanine couldn’t be isomerized into D-alanine anymore and the cell wall wouldn’t be assemble in the correct way.
D-alanine appears in peptidoglycan which is an important component of the bacterial cell wall. So the safety strain depends on the supplementation of alanine racemase on the plasmid which causes a good plasmid stability. If the bacteria cast the plasmid it will die because of the fact that L-alanine couldn’t be isomerized into D-alanine anymore and the cell wall wouldn’t be assemble in the correct way.
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==Theory==
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==Genetic Approach==
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==Results==
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==References==
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Revision as of 21:54, 29 September 2013



Safety Strain


Overview

IGEM Bielefeld 2913 Biosafety E.coli bewaffnet save ohne hintergrund.jpg

We produced a auxotrophic safety strain which was the basic for our biosafety system. The strain consists Δdadx and Δalr knockouts which prevents the expression of alanine racemase. Alanine racemase is an isomerase that catalyzes the chemical reaction from L-alanine to D-alanine. D-alanine appears in peptidoglycan which is an important component of the bacterial cell wall. So the safety strain depends on the supplementation of alanine racemase on the plasmid which causes a good plasmid stability. If the bacteria cast the plasmid it will die because of the fact that L-alanine couldn’t be isomerized into D-alanine anymore and the cell wall wouldn’t be assemble in the correct way.











Theory

Genetic Approach

Results

References







Contents

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