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Team:Heidelberg/Templates/Del week15 EF
From 2013.igem.org
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(Created page with "==08-08-2013== ===Amplification of FS_04 to FS_05; 6 kb=== [[File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png|150px|thumb|Amplifcation of DelAE and DelEF and DelOP; run a...") |
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==08-08-2013== | ==08-08-2013== | ||
| - | ===Amplification | + | ===Amplification from FS_04 to FS_05; 6 kb=== |
[[File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png|150px|thumb|Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)]] | [[File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png|150px|thumb|Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)]] | ||
Revision as of 23:41, 29 September 2013
08-08-2013
Amplification from FS_04 to FS_05; 6 kb
File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png
Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)
File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l cut.png
Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)
- Reaction
| what | µl |
|---|---|
| D. acidovorans SPH-1 | 1 |
| FS_04: (1/10) | 4 |
| FS_05: (1/10) | 4 |
| Phusion flash Master Mix | 10 |
- Conditions
| Biometra TProfessional Basic | ||
|---|---|---|
| Cycles | temperature [°C] | Time [s] |
| 1 | 98 | 10 |
| 30 | 98 | 1 |
| 58 | 5 | |
| 72 | 1:30 | |
| 1 | 72 | 7min |
| 1 | 12 | inf |
Results:
- Amplification of DelEF worked, though several side products and a smear occured
- bands were cut out and DNA purified using QIAquick Gel Extraction Kit
