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Team:Heidelberg/Templates/Del week11 pSB4K5
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(Created page with "==08-07-2013== ===Amplification from FS_01 to FS_16; 4.2 kb=== [[File:Heidelberg_20130708 DelAE DelBB DelG.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), ...")
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Revision as of 01:16, 30 September 2013
08-07-2013
Amplification from FS_01 to FS_16; 4.2 kb
- Reaction
(2x50µl)
| what | µl |
|---|---|
| Template pSB4K5 | 1 |
| FS_01: (1/10) | 2.5 |
| FS_16: (1/10) | 2.5 |
| Phusion Master Mix | 25 |
| DMSO | 2.5 |
| dd H2O | 16.5 |
- Conditions
| Biorad MyCycler | ||
|---|---|---|
| Cycles-PCR | temperature [°C] | Time [s] |
| 1 | 98 | 5 |
| 14 | 98 | 1 |
| 62 ↓ 0.5 | 5 | |
| 72 | 3:20 min | |
| 16 | 98 | 1 |
| 60 | 5 | |
| 72 | 3:20 min | |
| 1 | 72 | 12 min |
| 1 | 4 | inf |
Results:
- Amplification worked very well, we had bright bands on the right height.
- Bands were cut out and DNA purified using QIAquick Gel Extration Kit.
