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Team:Heidelberg/Templates/Del week11 AG
From 2013.igem.org
(Difference between revisions)
(Created page with "==12-07-2013== ===Amplification from FS_02 to FS_07; 16.4 kb=== [[File:20130712 DelAG.png|150px|thumb|PCR for amplification of DelAG (12.07;FS02+FS07); lane6=Ladder log2, lane7=...") |
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===Amplification from FS_02 to FS_07; 16.4 kb=== | ===Amplification from FS_02 to FS_07; 16.4 kb=== | ||
| - | [[File: | + | [[File:Heidelberg_20130712 DelAG.png|150px|thumb|PCR for amplification of DelAG (12.07;FS02+FS07); lane6=Ladder log2, lane7=Ladder 1kb base ruler, lane8=DelAG(12.07;FS02+FS_07) run at 100 V, 0.8 % gel (TAE)]] |
:'''Reaction''' | :'''Reaction''' | ||
| Line 9: | Line 9: | ||
! what !! µl | ! what !! µl | ||
|- | |- | ||
| - | | ''D. acidovorans'' || 1 | + | | ''D. acidovorans'' DSM-39 || 1 |
|- | |- | ||
| FS_02: (1/10) || 2 | | FS_02: (1/10) || 2 | ||
Revision as of 01:52, 30 September 2013
12-07-2013
Amplification from FS_02 to FS_07; 16.4 kb
- Reaction
| what | µl |
|---|---|
| D. acidovorans DSM-39 | 1 |
| FS_02: (1/10) | 2 |
| FS_07: (1/10) | 2 |
| Phusion flash Master Mix | 10 |
| DMSO | 1 |
| dd H2O | 4 |
- Conditions
| Biorad MyCycler | ||
|---|---|---|
| Cycles | temperature [°C] | Time [s] |
| 1 | 98 | 10 |
| 12 | 98 | 1 |
| 68 ↓ 0.5 | 5 | |
| 72 | 5:30 | |
| 18 | 98 | 1 |
| 66 | 5 | |
| 72 | 5:30 | |
| 1 | 72 | 15min |
| 1 | 12 | inf |
Results:
- only a smear occured, no specific product was amplified
- PCR will be repeated with a lower, constant annealing temperature
