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Team:Exeter/Results
From 2013.igem.org
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We have had marked success with our Green Light Module. Not only is the magenta pigment bright and evenly synthesised, but we have also managed to show magenta synthesis is inhibited by exposure to white light. We are currently working on showing that green light <i>specifically</i> switches off magenta production. | We have had marked success with our Green Light Module. Not only is the magenta pigment bright and evenly synthesised, but we have also managed to show magenta synthesis is inhibited by exposure to white light. We are currently working on showing that green light <i>specifically</i> switches off magenta production. | ||
| - | <gallery mode=packed heights=180px> | + | <gallery mode=packed heights=180px widths=200px> |
File:Centrifuged_universal_magenta.jpg|Universal showing our vibrant magenta pigment | File:Centrifuged_universal_magenta.jpg|Universal showing our vibrant magenta pigment | ||
File:Green_light_module_plates.jpg|Plates grown in the dark clearly show magenta pigment | File:Green_light_module_plates.jpg|Plates grown in the dark clearly show magenta pigment | ||
Revision as of 15:38, 30 September 2013
Green Light Module
We have had marked success with our Green Light Module. Not only is the magenta pigment bright and evenly synthesised, but we have also managed to show magenta synthesis is inhibited by exposure to white light. We are currently working on showing that green light specifically switches off magenta production.
 Universal showing our vibrant magenta pigment |
 Plates grown in the dark clearly show magenta pigment |
 Comparison of liquid cultures grown in dark vs. light |
Cyan pigment
Red Light Module output Brick with BglII and BamHI restriction sites
The two ends of GFP have been ligated together to form a complete, working protein in the successful Gibson assembly of our output bricks. GFP is constitutively expressed within DH5α cells as endogenous ENVZ phosporylates endogenous OmpR as a component of osmolarity sensing. Endogenous OmpR then binds to OmpF. Thus our output brick is working as expected. In order to test whether the output brick can be implemented into the Red light pathway and ENVZ- strain should be used to avoid false positives.
Post-Freeze Work
Blue Light Module
Red Light Module
(Cyan working in EnvZ deficient cells)
With many thanks to our sponsors:
