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Team:Groningen/Project/Backbone
From 2013.igem.org
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| - | To transform <i>B. subtilis</i> with our developed biobricks we have improved the <i>amyE</i> intergrational backbone from Munich's iGEM team 2012 (<a href="http://parts.igem.org/Part:BBa_K823023">BBa_K823023</a>) that had no inducible promoter. We've added the HyperSpank IPTG inducible promoter from Rudner's Lab 2004. This promoter is placed right in front of the prefix, so any biobrick can be inserted in this backbone (<a href="http://parts.igem.org/Part:BBa_K1085014">BBa_K1085014</a>). | + | To transform <i>B. subtilis</i> with our developed biobricks we have improved the <i>amyE</i> intergrational backbone from Munich's iGEM team 2012 (<a href="http://parts.igem.org/Part:BBa_K823023">BBa_K823023</a>) that had no inducible promoter. We've added the HyperSpank IPTG inducible promoter from Rudner's Lab 2004. This promoter is placed right in front of the prefix, so any biobrick can be inserted in this backbone (<a href="http://parts.igem.org/Part:BBa_K1085014">BBa_K1085014</a>)and can be induced with IPTG.<p> |
| + | In figure 1 a close-up of the promoter is shown. Two lacO operaters | ||
Revision as of 19:40, 30 September 2013
Backbone
To transform B. subtilis with our developed biobricks we have improved the amyE intergrational backbone from Munich's iGEM team 2012 (BBa_K823023) that had no inducible promoter. We've added the HyperSpank IPTG inducible promoter from Rudner's Lab 2004. This promoter is placed right in front of the prefix, so any biobrick can be inserted in this backbone (BBa_K1085014)and can be induced with IPTG.
In figure 1 a close-up of the promoter is shown. Two lacO operaters
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| Figure 1: Hy_Spank promoter |
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| Figure 2: |
Promoter activity
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Figure 3: The intensity in of ~40 cells in the GFP-channel, were analyzed from two pictures.
The average intensity (AU) from the cells are plotted above with the standard deviation. |
GFPmg and GFP (BBa_E0840)
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Figure 4: |
