Team:DTU-Denmark/pBAD SPL

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(pBAD synthetic promoter library)
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== pBAD synthetic promoter library ==
== pBAD synthetic promoter library ==
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As a tool for expressing lethal proteins in ''E. coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL (link)].
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As a tool for expressing lethal proteins in ''E. coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the [https://2010.igem.org/Team:DTU-Denmark/SPL DTU iGEM team from 2010].
== Method ==
== Method ==

Revision as of 20:25, 30 September 2013

pBAD SPL

Contents

pBAD synthetic promoter library

As a tool for expressing lethal proteins in E. coli we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010.

Method

USER cloning for SPL

Results

Dtu-Fss-plot-col2.png Dtu-Fss-plot-col3.png Dtu-Fss-plot-col4.png Dtu-Fss-plot-col12.png Dtu-Fss-plot-col10.png Dtu-Fss-plot-col9.png Dtu-Fss-plot-col8.png Dtu-Fss-plot-col13.png Dtu-Fss-plot-col15.png Dtu-Fss-plot-col18.png Dtu-Fss-plot-col19.png Dtu-Fss-plot-col29.png Dut-Fss-plot-col26.png Dtu-Fss-plot-col22.png Dtu-Fss-plot-col33.png Dtu-Fss-plot-col34.png Dtu-Fss-plot-col25.png Dtu-Fss-plot-conref.png

Example of use

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See also "Hello World project".