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Team:Bonn/Project/
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<img src="https://static.igem.org/mediawiki/2013/7/79/Bonn_sponsors_limes.gif" class="bottom-sponsor" height="50px" id="sponsor-limes"> | <img src="https://static.igem.org/mediawiki/2013/7/79/Bonn_sponsors_limes.gif" class="bottom-sponsor" height="50px" id="sponsor-limes"> | ||
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| - | <div class="subpage-head-link"><a href="javascript:history.back();">Back</a></br> Category: Project</div> | + | <div class="subpage-head-link"><a href="javascript:history.back();">Back</a></br> <span id="type">Category: Project</span></div> |
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= Overview = | = Overview = | ||
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| - | Making versatile control of biological machines easily possible for everybody we engineer a system for | + | Making versatile control of biological machines easily possible for everybody we engineer a system for |
light dependend control of protein activity per protein degradation.</br> | light dependend control of protein activity per protein degradation.</br> | ||
| - | Using the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused | + | Using the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused |
| - | protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is | + | protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is |
activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br> | activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br> | ||
| - | Modelling of the light dependency of protein degradation will even make simple and accurate control of | + | Modelling of the light dependency of protein degradation will even make simple and accurate control of |
protein activity for any desired level of activity possible.</br> | protein activity for any desired level of activity possible.</br> | ||
As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br> | As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br> | ||
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Revision as of 06:04, 1 October 2013
Back Category: Project
Overview
Making versatile control of biological machines easily possible for everybody we engineer a system for
light dependend control of protein activity per protein degradation.
Using the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused
protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation.
Modelling of the light dependency of protein degradation will even make simple and accurate control of
protein activity for any desired level of activity possible.
As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.
