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Team:TU Darmstadt/protocols
From 2013.igem.org
(Difference between revisions)
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | ||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest] | ||
| - | |||
* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | ||
| + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification Wizard SV Gel and PCR Clean-Up System] | ||
=== [http://en.wikipedia.org/wiki/Protein Protein] === | === [http://en.wikipedia.org/wiki/Protein Protein] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page Spectra measurement] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page Spectra measurement] | ||
Revision as of 14:24, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- Protein expression of LssmOrange and mKate
In vitro
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- PCR
- PureYield Plasmid Miniprep System
- Restriction digest
- [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA]
- Wizard SV Gel and PCR Clean-Up System
