"
Team:Groningen/8 July 2013
From 2013.igem.org
(Difference between revisions)
(Created page with "'''Mirjam''' <br/> Measured the concentration of the promoter: 20,1 ng/ul.") |
|||
| Line 1: | Line 1: | ||
'''Mirjam''' | '''Mirjam''' | ||
<br/> Measured the concentration of the promoter: 20,1 ng/ul. | <br/> Measured the concentration of the promoter: 20,1 ng/ul. | ||
| + | |||
| + | Made a restriction digestion for the promoter and the BBa_K823023 backbone. | ||
| + | <br/>The following protocol is used: 159-200 ng DNA, 0.5 ul BSA, 2.5 ul EcoRI buffer, 1 ul BamHI, 0.5 ul EcoRI, adjusted to 20 ul with MQ water. The reaction is incubated for 2 hours at 37 degrees Celsius. | ||
Revision as of 08:38, 8 July 2013
Mirjam
Measured the concentration of the promoter: 20,1 ng/ul.
Made a restriction digestion for the promoter and the BBa_K823023 backbone.
The following protocol is used: 159-200 ng DNA, 0.5 ul BSA, 2.5 ul EcoRI buffer, 1 ul BamHI, 0.5 ul EcoRI, adjusted to 20 ul with MQ water. The reaction is incubated for 2 hours at 37 degrees Celsius.
