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Team:Groningen/8 July 2013
From 2013.igem.org
(Difference between revisions)
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Made a restriction digestion for the promoter and the BBa_K823023 backbone. | Made a restriction digestion for the promoter and the BBa_K823023 backbone. | ||
<br/>The following protocol is used: 159-200 ng DNA, 0.5 ul BSA, 2.5 ul EcoRI buffer, 1 ul BamHI, 0.5 ul EcoRI, adjusted to 20 ul with MQ water. The reaction is incubated for 2 hours at 37 degrees Celsius. | <br/>The following protocol is used: 159-200 ng DNA, 0.5 ul BSA, 2.5 ul EcoRI buffer, 1 ul BamHI, 0.5 ul EcoRI, adjusted to 20 ul with MQ water. The reaction is incubated for 2 hours at 37 degrees Celsius. | ||
| + | |||
| + | Run a gel for 10 minutes 90V to reveal if the compounds are digested. | ||
Revision as of 10:37, 8 July 2013
Mirjam
Measured the concentration of the promoter: 20,1 ng/ul.
Made a restriction digestion for the promoter and the BBa_K823023 backbone.
The following protocol is used: 159-200 ng DNA, 0.5 ul BSA, 2.5 ul EcoRI buffer, 1 ul BamHI, 0.5 ul EcoRI, adjusted to 20 ul with MQ water. The reaction is incubated for 2 hours at 37 degrees Celsius.
Run a gel for 10 minutes 90V to reveal if the compounds are digested.
