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content.i = 2;

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content.parents=[];

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content.parents=1;

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content.childs=[2,17];

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content.childs=[3,6];

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content.titleShort = "~~Proteinaktivität~~";

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content.titleShort = "Protein regulation";

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content.titleLong = "";

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content.titleLong = "Protein regulation mechanisms";

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content.summary= "";

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content.summary= "We compare different regulation systems, focused on advantages and disadvanteges for scienticif use";

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content.text= "";

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content.text= "To understand the role of a specific gene or DNA region is one of the big challenges in modern research. Our system, which allows the fast and convenient elimination of defined proteins, is a new improved technique, with many advantages. The following table compares methods, advantages and disadvantages of several popular regulation methods.<table><tr><td>Regulation system</td><td>Approach of regulation</td><td>Activating / repressing</td><td>advantage</td><td>disadvantage</td></tr><tr><td>Knock-In</td><td>Insert of DNA</td><td>activating</td><td>gain of function; high difference of activity</td><td>No ON/OFF system</td></tr><tr><td>Knock-Out</td><td>Deletion of DNA</td><td>desactivating</td><td>0% Protein in organism</td><td>no ON/OFF system</td></tr><tr><td>Knock-Down</td><td>Inhibition of RNA</td><td>Repressing</td><td>inducible</td><td>Eypensive; small activity difference</td></tr><tr><td>Riboswitches</td><td>mRNA structure; Transcription & Translation</td><td>Activating/ repressing/ degradation</td><td>Multiple aproaches and effects</td><td>harder to modulate<td></tr><tr><td>Zymogen-like</td><td>Protein structure</td><td>Activating</td><td>Inducible</td><td>No deactivation</td></tr><tr><td>Operon</td><td>Transcription</td><td>Inductive (substrate)/ repressing (product)</td><td>Self-regulating in organisms</td><td>Not usable for every protein</td></tr><tr><td>TALEs</td><td>Transcription</td><td>Both</td><td>Can bind several effectors</td><td>Very specific</td></tr><tr><td>Zinc finger<td>Transcription</td><td>Both</td><td>Can bind several effectors</td><td>Very specific</td></tr><tr><td>Direct regulation</td><td>Protein affinity</td><td>Both</td><td>Very fast</td><td>Too specific for easy, general use</td></tr><tr><td>ClpXP protease system</td><td>Protein degradation</td><td>'Repressing'</td><td>Very fast & transferable</td><td> No obvious disadvantage</td></tr></table></break>After the comparison of all the different Protein regulation mechanisms, our team decided to make use of a protein degradation system. The reason was that we wanted to create a system that has an immediate effect and can be used to investigate functions of every protein.";

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content.type="Background";

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content.type="Background";

break;

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case 3:

@@ Line 33: / Line 33: @@
 content.i = 2;
-content.parents=[];
+content.parents=1;
-content.childs=[2,17];
+content.childs=[3,6];
-content.titleShort = "Proteinaktivität";
+content.titleShort = "Protein regulation";
-content.titleLong = "";
+content.titleLong = "Protein regulation mechanisms";
-content.summary= "";
+content.summary= "We compare different regulation systems, focused on advantages and disadvanteges for scienticif use";
-content.text= "";
+content.text= "To understand the role of a specific gene or DNA region is one of the big challenges in modern research. Our system, which allows the <b>fast</b> and <b>convenient</b> elimination of defined proteins, is a new improved technique, with many advantages. The following table compares methods, advantages and disadvantages of several popular regulation methods.</br></br><table><tr><td><i>Regulation system</i></td><td><i>Approach of regulation</i></td><td><i>Activating / repressing</i></td><td><i>advantage</i></td><td><i>disadvantage</i></td></tr><tr><td><b>Knock-In</b></td><td>Insert of DNA</td><td>activating</td><td>gain of function; high difference of activity</td><td>No ON/OFF system</td></tr><tr><td><b>Knock-Out</b></td><td>Deletion of DNA</td><td>desactivating</td><td>0% Protein in organism</td><td>no ON/OFF system</td></tr><tr><td><b>Knock-Down<b></td><td>Inhibition of RNA</td><td>Repressing</td><td>inducible</td><td>Eypensive; small activity difference</td></tr><tr><td><b>Riboswitches</b></td><td>mRNA structure; Transcription & Translation</td><td>Activating/ repressing/ degradation</td><td>Multiple aproaches and effects</td><td>harder to modulate<td></tr><tr><td><b>Zymogen-like</b></td><td>Protein structure</td><td>Activating</td><td>Inducible</td><td>No deactivation</td></tr><tr><td><b>Operon</b></td><td>Transcription</td><td>Inductive (substrate)/ repressing (product)</td><td>Self-regulating in organisms</td><td>Not usable for every protein</td></tr><tr><td><b>TALEs</b></td><td>Transcription</td><td>Both</td><td>Can bind several effectors</td><td>Very specific</td></tr><tr><td><b>Zinc finger</b><td>Transcription</td><td>Both</td><td>Can bind several effectors</td><td>Very specific</td></tr><tr><td><b>Direct regulation</b></td><td>Protein affinity</td><td>Both</td><td>Very fast</td><td>Too specific for easy, general use</td></tr><tr><td><b>ClpXP protease system</b></td><td>Protein degradation</td><td>'Repressing'</td><td>Very fast & transferable</td><td> No obvious disadvantage</td></tr></table></break></br>After the comparison of all the different Protein regulation mechanisms, our team decided to make use of a protein degradation system. The reason was that we wanted to create a system that has an immediate effect and can be used to investigate functions of every protein.";
 content.type="Background";
 break;
 case 3:

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