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Team:DTU-Denmark/Notebook/8 July 2013
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(Created page with "=208= ==Main purpose== Run gel with 1 AMO, 10 AMO, 20 AMO genes. Purification and extraction of the gel for 10 AMO. Run 2nd gel with all the sample of 10 AMO. ==Who was in the l...")
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Revision as of 15:37, 8 July 2013
Contents |
208
Main purpose
Run gel with 1 AMO, 10 AMO, 20 AMO genes. Purification and extraction of the gel for 10 AMO. Run 2nd gel with all the sample of 10 AMO.
Who was in the lab
Kristian, Ariadni, Gosia
Procedure
Run 1st gel:
- 1ul of dye
- 5ul of sample
- 5ul Kb ladder
Purification of first gel for 10 AMO according to the protocol QIA gel extraction protocol. (given in the Kit)
Run 2nd gel:
- 9ul of dye
- around 20 ul of each sample
- 12 ul of ladder
Purification of second gel according to the same protocol.
Measure the concentration of the purified AMO using Nanodrop (pouring together both samples.
