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Exeter/7 July 2013
From 2013.igem.org
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(→Making standard liquid cultures of our transformed cells) |
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- 5 ml LB broth | - 5 ml LB broth | ||
| - | - 5 | + | - 5 µl antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone) |
| - | We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 | + | We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 µl of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes. |
We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube. | We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube. | ||
| - | The tubes were incubated overnight in a shaking incubator at | + | The tubes were incubated overnight in a shaking incubator at 37 °C. |
Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | ||
Latest revision as of 20:47, 1 October 2013
Making standard liquid cultures of our transformed cells
In each 10 ml Falcon, we need:
- 5 ml LB broth
- 5 µl antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 µl of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes.
We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube.
The tubes were incubated overnight in a shaking incubator at 37 °C.
Take me back to the notebook.
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