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Template:Team:Bonn:NetworkData
From 2013.igem.org
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case 10: | case 10: | ||
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content.i = 10; | content.i = 10; | ||
content.parents=[6]; | content.parents=[6]; | ||
content.childs=[]; | content.childs=[]; | ||
| - | content.titleShort = " | + | content.titleShort = "Knock-down"; |
| - | content.titleLong = ""; | + | content.titleLong = "Genetical knock-down by RNA-inference"; |
content.summary= ""; | content.summary= ""; | ||
| - | content.text= ""; | + | content.text= "The genetical "Knock Down" by RNAi does not affect directly transcription or DNA, but it is based on RNA level. Here a DNA construct, which codes for small double-stranded interfering RNAs (siRNA), is inserted into the cell. The siRNA is processed by the RNA-induced silencing complex (RISC), described by Pratt and MacRae in 2009. The siRNA is complementary to the target mRNA and therefore forms a double strand with it and the RISC. After the RISC localizes to the target mRNA, the RNA is cleaved by a ribonuclease. Hence the mRNA of the knockdown gene is degraded and no protein can be produced.<sup> <a href=#10.1>[10.1]</a> </sup></br><p> <a name=10.1>[10.1]</a> <a href='http://www.jbc.org/content/284/27/17897.long'>The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine, Ashley J. Pratt and Ian J. MacRae1 </a></p>"; |
content.type="Background"; | content.type="Background"; | ||
break; | break; | ||
Revision as of 22:37, 1 October 2013
