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{

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1> Name of participants: Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> Assembly: 353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old) Trasformation: 111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP) 353. pSBLb4E15-red PCR-purification: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) DpnI-treatment: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'

+

}

+

else if(id == 'd201395')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1> Name of participants: Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 335. pSB3K3-J23101-HIs-STS <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201396')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> PCR-purification and recircularization: Mutagenesis'd pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30) O/N: 111.7 pSBLb4C15-red* *to do a new plasmid preparation since old may have corrupt red-cassette/contamination) Streak from frozen stock: 111.7 pSBLb4C15-red* * to test if red-cassette has been damaged, or possibly the stock contaminated. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201397')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1> Name of participants: Mikael Strandgren <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work:</h2> Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE) 355.2 pSB3K3-CP29-mCherry 355.3 pSB3K3-CP29-mCherry 357.3 pSB3K3-CP30-usp45-mCherry 357.4 pSB3K3-CP30-usp45-mCherry 358.1 pSB3K3-CP29-usp45-mCherry 358.2 pSB3K3-CP29-usp45-mCherry 363. pSB3K3-CP30-tspurple 364. pSB3K3-CP29-tspurple 365. pSB3K3-CP11-tspurple 366. pSB3K3-CP30-amilCP 367. pSB3K3-CP29-amilCP 368. pSB3K3- CP11-amilCP 369. pSB3K3-CP30-aeblue 370. pSB3K3-CP29-aeblue 371. pSB3K3-CP11-aeblue Assembly: 407. pSB1C3-CP29-Mcherry* 408. pSB1C3-CP30-usp45-mCherry* 409. pSB1C3-CP29-usp45-mCherry* 410. pSB1C3-CP30-tsPurple* 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP* 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue* 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old) * pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today. Transformation 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette. O/N: 111.7 pSBLb4C15-red <h2>Results: </h2> O/N: 111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today. Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl 355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl 355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl 357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl 357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl 358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl 358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl 363. pSB3K3-CP30-tspurple: 75.5 ng/µl 364. pSB3K3-CP29-tspurple: 43.9 ng/µl 365. pSB3K3-CP11-tspurple: 47.3 ng/µl 366. pSB3K3-CP30-amilCP: 74.5 ng/µl 367. pSB3K3-CP29-amilCP: 77.2 ng/µl 368. pSB3K3- CP11-amilCP: 34.3 ng/µl 369. pSB3K3-CP30-aeblue: 37.4 ng/µl 370. pSB3K3-CP29-aeblue: 60.3 ng/µl 371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'

+

}

+

else if(id == 'd201398')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-08</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work: </h2> Transformations: 411. pSB1C3-CP29-tsPurple * 412. pSB1C3-CP11-tsPurple * 414. pSB1C3-CP29-amilCP * 415. pSB1C3-CP11-amilCP * 417. pSB1C3-CP29-aeBlue * 418. pSB1C3-CP11-aeBlue * * The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good. ON: 353. pSBLB4E15-red <h2>Results: </h2> Transformation 411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today. 412. pSB1C3-CP11-tsPurple: “ 414. pSB1C3-CP29-amilCP “ 415. pSB1C3-CP11-amilCP “ 417. pSB1C3-CP29-aeBlue “ 418. pSB1C3-CP11-aeBlue “ 419. pSBLbEcE-red: Growth covering the plates, only white colonies. pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'

}

else if(id == 'd2013919')

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   {
 	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>Assembly:</b><br>353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old)<br><br><b>Trasformation:</b><br>111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP)<br>353. pSBLb4E15-red <br><br><b>PCR-purification:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)<br><br><b>DpnI-treatment:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'
+ }
+      else if(id == 'd201395')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1><br><b>Name of participants:</b> Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201396')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>PCR-purification and recircularization:</b><br>Mutagenesis'd pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30)<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red*<br><i>*to do a new plasmid preparation since old may have corrupt red-cassette/contamination)</i><br><br><b>Streak from frozen stock:</b><br>111.7 pSBLb4C15-red*<br><i>* to test if red-cassette has been damaged, or possibly the stock contaminated.</i><br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201397')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1><br><br><b>Name of participants:</b> Mikael Strandgren<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work:</h2> <br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE) <br>355.2 pSB3K3-CP29-mCherry<br>355.3 pSB3K3-CP29-mCherry<br>357.3 pSB3K3-CP30-usp45-mCherry<br>357.4 pSB3K3-CP30-usp45-mCherry<br>358.1 pSB3K3-CP29-usp45-mCherry<br>358.2 pSB3K3-CP29-usp45-mCherry<br>363. pSB3K3-CP30-tspurple<br>364. pSB3K3-CP29-tspurple<br>365. pSB3K3-CP11-tspurple<br>366. pSB3K3-CP30-amilCP<br>367. pSB3K3-CP29-amilCP<br>368. pSB3K3- CP11-amilCP<br>369. pSB3K3-CP30-aeblue<br>370. pSB3K3-CP29-aeblue<br>371. pSB3K3-CP11-aeblue<br><br><b>Assembly:</b><br>407. pSB1C3-CP29-Mcherry*<br>408. pSB1C3-CP30-usp45-mCherry*<br>409. pSB1C3-CP29-usp45-mCherry*<br>410. pSB1C3-CP30-tsPurple*<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP* <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue*<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old)<br>* pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today.<br><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>417. pSB1C3-CP29-aeBlue <br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red <br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette.<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red<br><br><h2>Results: </h2><br><b>O/N:</b><br>111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today.<br><br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl<br>355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl<br>355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl<br>357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl<br>357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl<br>358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl<br>358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl<br>363. pSB3K3-CP30-tspurple: 75.5 ng/µl<br>364. pSB3K3-CP29-tspurple: 43.9 ng/µl<br>365. pSB3K3-CP11-tspurple: 47.3 ng/µl<br>366. pSB3K3-CP30-amilCP: 74.5 ng/µl<br>367. pSB3K3-CP29-amilCP: 77.2 ng/µl<br>368. pSB3K3- CP11-amilCP: 34.3 ng/µl<br>369. pSB3K3-CP30-aeblue: 37.4 ng/µl<br>370. pSB3K3-CP29-aeblue: 60.3 ng/µl<br>371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'
+ }
+      else if(id == 'd201398')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-08</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work: </h2><br><b>Transformations:</b><br>411. pSB1C3-CP29-tsPurple *<br>412. pSB1C3-CP11-tsPurple *<br>414. pSB1C3-CP29-amilCP *<br>415. pSB1C3-CP11-amilCP *<br>417. pSB1C3-CP29-aeBlue *<br>418. pSB1C3-CP11-aeBlue *<br>* The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good.<br><br><b>ON:</b><br>353. pSBLB4E15-red<br><br><h2>Results: </h2><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today.<br>412. pSB1C3-CP11-tsPurple: 			“ <br>414. pSB1C3-CP29-amilCP 			“<br>415. pSB1C3-CP11-amilCP 			“<br>417. pSB1C3-CP29-aeBlue 			“<br>418. pSB1C3-CP11-aeBlue			“<br>419. pSBLbEcE-red: Growth covering the plates, only white colonies.<br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'
   }
   else if(id == 'd2013919')

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