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From 2013.igem.org

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Revision as of 13:06, 2 October 2013

{ "date" : "2013-07-30", "author" : "gabriele", "title" : "pLAC to SAMsynthetase", "content" : "I digested pSB1C3+pLac with PstI-HF and SpeI and SAMsynthetase (PCR insert) with PstI-HF and XbaI, using the 'short' protocol (1.5 µg of insert, 1 µg of plasmid, for 2h at 37°C). Then I treated as usual the plasmid with SAP and the insert with DpNI and purified them. The yields of the purification was too low, I was able to perform only Ctrl, 1:1 and 1:2 ligations.", "tags" : "SAMsynthetase-pLac" }