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Team:UNITN-Trento/Notebook/Labposts/08/54
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(Created page with "{ "date" : "2013-08-01", "author" : "gabriele", "title" : "pLAC to SAMsynthetase - Ep. III", "content" : "<html>I miniprepped the eight inocula from yesterday (great yields, ...")
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(Created page with "{ "date" : "2013-08-01", "author" : "gabriele", "title" : "pLAC to SAMsynthetase - Ep. III", "content" : "<html>I miniprepped the eight inocula from yesterday (great yields, ...")
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Revision as of 13:06, 2 October 2013
{ "date" : "2013-08-01", "author" : "gabriele", "title" : "pLAC to SAMsynthetase - Ep. III", "content" : "I miniprepped the eight inocula from yesterday (great yields, the minimum was 206.3 ng/µl) and screened them with EcoRI-HF and PstI-HF. Five inocula (11A-C, 12B-C-D) were positive!!!
Agarose Gel
![](\"https://static.igem.org/mediawiki/2013/4/42/Tn-20130801_ggcm_SAMsynthINpSB1C3withPlac_GOTITfuckyeah.jpg\")
One sample was sent to be sequenced.",
"tags" : "SAMsynthetase-pLac"
}
| Loading scheme |
|---|
| 11A |
| 11B |
| 11C |
| 11D |
| Empty |
| 1kb ladder |
| Empty |
| 12A |
| 12B |
| 12C |
| 12D |
