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Team:Groningen/9 July 2013
From 2013.igem.org
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spotted transmuted cells and put them in a 37 C shaker (at 11:35) | spotted transmuted cells and put them in a 37 C shaker (at 11:35) | ||
| + | Cells need to grow over night. | ||
| + | |||
| + | 15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low) | ||
Revision as of 13:03, 9 July 2013
Mirjam
PCR made for silk without strep-tags.
Gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.
Inne
spotted transmuted cells and put them in a 37 C shaker (at 11:35) Cells need to grow over night.
15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)
