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Team:METU Turkey/index.html
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<title>Home</title> | <title>Home</title> | ||
<!-- CSS --> | <!-- CSS --> | ||
| - | <link rel="stylesheet" type="text/css" href="http://wiki.metuigem.com/css/site_global.css? | + | <link rel="stylesheet" type="text/css" href="http://wiki.metuigem.com/css/site_global.css?3837790900"/> |
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<li class="MenuItemContainer clearfix colelem" id="u4853"><!-- horizontal box --> | <li class="MenuItemContainer clearfix colelem" id="u4853"><!-- horizontal box --> | ||
<a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix grpelem" id="u4856" href="kill-switch.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u4858-4"><!-- content --><p>Kill Switch</p></div></a> | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix grpelem" id="u4856" href="kill-switch.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u4858-4"><!-- content --><p>Kill Switch</p></div></a> | ||
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| - | <iframe width="420" height="315" src="//www.youtube.com/embed/ | + | <iframe width="420" height="315" src="//www.youtube.com/embed/kJk4OpdEKWE" frameborder="0" allowfullscreen></iframe> |
</div> | </div> | ||
| - | < | + | <img class="grpelem" id="u5138-13" src="http://ghucuk.netii.net/images/u5138-13.png" alt="Our Project Description:
Taking a major role in pollination, bees are one of the most important organisms within an ecosystem. However their populations are in serious decline. Colony Collapse Disorder has been found as the most common cause of the disappearance of bees in large numbers. Some pathogens like parasitic fungi and a pesticide called imidacloprid are the leading reasons of CCD. In this study, our aim is to decrease the number of hives affected by chemical compounds such as imidacloprid. Our plan is to turn the mutualistic bacteria living in bees' guts into a shield mechanism to protect the bees against these factors. A protein CYP6G1 found in Drosophilia melanogaster has the ability to degrade imidacloprid into harmless substances. Moreover, coumaric acid increases the general immunity of bees against harmful components and parasitic organisms and we aim to increase the level of coumaric acid in bees' guts. The main objective of this study is the transformation of the genes coding for these two proteins to Bacillus subtilis, which mutualistically live in bees' guts." width="449" height="288"/><!-- rasterized frame --> |
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<div class="ContainerGroup clearfix" id="u3280"><!-- stack box --> | <div class="ContainerGroup clearfix" id="u3280"><!-- stack box --> | ||
<div class="Container clearfix grpelem" id="u3286"><!-- group --> | <div class="Container clearfix grpelem" id="u3286"><!-- group --> | ||
| - | <div class="clearfix grpelem" id="u3287- | + | <div class="clearfix grpelem" id="u3287-8"><!-- content --> |
| - | <p id="u3287-4"><span><span class="actAsDiv normal_text" id="u3288"><!-- content --><span class="actAsDiv museBGSize" id="u3289"><!-- image --></span></span></span><span> </span><span id="u3287-3"> | + | <p id="u3287-4"><span><span class="actAsDiv normal_text" id="u3288"><!-- content --><span class="actAsDiv museBGSize" id="u3289"><!-- image --></span></span></span><span> </span><span id="u3287-3">As a well-known fact, bees take the major part in pollination, which leads to give fruit, while preparing honey. During the last few decades, some powerful insecticides like imidacloprid were developed to fight with harmful insects; however, the ability to kill almost all insects started to affect other insects, which they must not have affected from the beginning.</span></p> |
| - | <p id="u3287- | + | <p id="u3287-6">This was the start point of our project. We, as a group formed by mostly biological sciences students, knew that no ecological system can maintain without bees. When we looked into the source of the problem, it was clearly revealed that we couldn’t stop usage of imidacloprid. Then, we came up with an idea to protect bees against existing imidacloprid. Having known that drosophila has some sort of immunity to this insecticide, we planned to use it.</p> |
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<div class="Container invi grpelem" id="u3281"><!-- simple frame --></div> | <div class="Container invi grpelem" id="u3281"><!-- simple frame --></div> | ||
<div class="Container invi clearfix grpelem" id="u3291"><!-- group --> | <div class="Container invi clearfix grpelem" id="u3291"><!-- group --> | ||
| - | <div class="clearfix grpelem" id="u3292- | + | <div class="clearfix grpelem" id="u3292-9"><!-- content --> |
| - | <p id="u3292-3"><span id="u3292"><span class="actAsDiv normal_text" id="u3293"><!-- content --><span class="actAsDiv museBGSize" id="u3294"><!-- image --></span></span></span><span id="u3292-2"> | + | <p id="u3292-3"><span id="u3292"><span class="actAsDiv normal_text" id="u3293"><!-- content --><span class="actAsDiv museBGSize" id="u3294"><!-- image --></span></span></span><span id="u3292-2"> In this year, in the kill switch part of our project, we use an mRNA interferase, MazF, which cleaves mRNA’s at specific sequences. In our kill switch, we used anti-sense RNA principle as a template. According to this principle, MazF, which is constantly produced via a constitutive promoter, is got inactivated by Anti-MazF construct. In order to trigger this mechanism, we used IPTG, a harmless molecule for the bee which at the same time does not appear in the honey too. When IPTG is present in the environment, LacI is inhibited by IPTG and therefore promoter gets activated. With the activated promoter of it, Anti-MazF is produced and inactive MazF. As long as IPTG exists, MazF gets inactivated continuously; therefore, the bacteria maintain their lives. On the other hand, if bacteria exists in a IPTG-free environment, Anti-MazF producing stops, which leads to MazF producing and bacteria get killed by MazF.</span></p> |
| - | <p id="u3292-5"> | + | <p id="u3292-5">In this circuit, we used the parts K143053 as a constitutive promoter and SpoVG RBS due to their more efficient works in Bacillus subtilis. Besides these two, we used the part I732820 to produce LacI, but we had to change the RBS of this system since RBSelowitz is less effective in Bacillus then RBSspoVG. With this change in the activity of the RBS, we can compete with the production of MazF.</p> |
| + | <p id="u3292-6"> </p> | ||
| + | <p id="u3292-7"> </p> | ||
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| + | <a class="twitter-timeline" href="https://twitter.com/iGEM_METU2013" data-widget-id="372850246176362496">Tweets by @iGEM_METU2013</a> | ||
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| + | <div class="SSSlideLink clearfix grpelem" id="u7427"><!-- image --> | ||
| + | <div id="u7427_clip"> | ||
| + | <img class="position_content" id="u7427_img" src="http://ghucuk.netii.net/images/img_991665x43.jpg" alt="" width="65" height="43"/> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="popup_anchor" id="u6679popup"> | ||
| + | <div class="SlideShowCaptionPanel clearfix" id="u6679"><!-- stack box --> | ||
| + | <div class="SSSlideCaption clearfix grpelem" id="u7304-4"><!-- content --> | ||
| + | <p>First meeting of METU iGEM '13 team</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7007-4"><!-- content --> | ||
| + | <p>It was a snowy day when we fell in love with iGEM.</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7016-4"><!-- content --> | ||
| + | <p>The aseptic conditions are satisfied.</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7025-4"><!-- content --> | ||
| + | <p>Researching for our project.</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7034-4"><!-- content --> | ||
| + | <p>Optimizations for lab work</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7043-4"><!-- content --> | ||
| + | <p>Collaboration with ITU iGEM team @ Istanbul</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7052-4"><!-- content --> | ||
| + | <p>Collaboration with ITU iGEM team @ Istanbul</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7061-4"><!-- content --> | ||
| + | <p>Seminar about synthetic biology given by us to METU College students</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7070-4"><!-- content --> | ||
| + | <p>Hanging out with Baskent Uni iGEM team</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7079-4"><!-- content --> | ||
| + | <p>Modelling team is working, not playing LoL.</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7088-4"><!-- content --> | ||
| + | <p>Participating in Treasure Hunt '13 in METU</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7097-4"><!-- content --> | ||
| + | <p>Modelling team saying goodbye to our captain, Buse Reis, who kept in touch with us in Cambridge.</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7106-4"><!-- content --> | ||
| + | <p>Strengthening our bonds by group activities</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7115-4"><!-- content --> | ||
| + | <p>The collaboration of all iGEM teams in Turkey. We presented our projects to each other and our advisors to improve them and to get feedback.</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7124-4"><!-- content --> | ||
| + | <p>Making an introduction to lab works for ITU MOBGAM team</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7142-4"><!-- content --> | ||
| + | <p>Making an introduction to lab works for ITU MOBGAM team</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7133-4"><!-- content --> | ||
| + | <p>Safety first :D</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7151-4"><!-- content --> | ||
| + | <p>#direnplate</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7160-4"><!-- content --> | ||
| + | <p>One of our meetings with Assoc. Prof. Dr. Mesut MUYAN</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7169-4"><!-- content --> | ||
| + | <p>Collaboration and education with mechanical engineers of METU</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7178-4"><!-- content --> | ||
| + | <p>Ancient autoclave found in METU Biological Sciences building</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7187-4"><!-- content --> | ||
| + | <p>Planning the circuits</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7196-4"><!-- content --> | ||
| + | <p>Hosting future iGEMers from Güven Eğitim & Sağlık Vakfı in METU</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7205-4"><!-- content --> | ||
| + | <p>Young iGEMers appreciating science</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7214-4"><!-- content --> | ||
| + | <p>Our captain after doing hard laboratory work :D</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7223-4"><!-- content --> | ||
| + | <p>SCIENCE B*TCH</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7241-4"><!-- content --> | ||
| + | <p>Enjoying our bee cookies aseptically in lab</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7286-4"><!-- content --> | ||
| + | <p>Thanks to Nihal'in Elinden for cookies</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7250-4"><!-- content --> | ||
| + | <p>Enjoying our bee cookies not so very aseptically in lab</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7509-4"><!-- content --> | ||
| + | <p>Falcon quality control :p</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7268-4"><!-- content --> | ||
| + | <p>Modelling team at work</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7277-4"><!-- content --> | ||
| + | <p>Raising awareness about synthetic biology in METU</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7295-4"><!-- content --> | ||
| + | <p>Brainstorming for the project</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7376-4"><!-- content --> | ||
| + | <p>SynBio Party poster</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7313-4"><!-- content --> | ||
| + | <p>SynBio Party cocktails</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7322-4"><!-- content --> | ||
| + | <p>SynBio Party people having fun</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7331-4"><!-- content --> | ||
| + | <p>SynBio Party</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7340-4"><!-- content --> | ||
| + | <p>SynBio Party 300 -500</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7349-4"><!-- content --> | ||
| + | <p>SynBio Party</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7358-4"><!-- content --> | ||
| + | <p>SynBio Party :D</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7367-4"><!-- content --> | ||
| + | <p>SynBio Party which made us some funds</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7385-4"><!-- content --> | ||
| + | <p>Synthetic Biology Day Crew</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7421-4"><!-- content --> | ||
| + | <p>Our beloved participants in Synthetic Biology day</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7439-4"><!-- content --> | ||
| + | <p>Synthetic Biology Day Crew and Participants</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7412-4"><!-- content --> | ||
| + | <p>iGEM Stand in METU</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7394-4"><!-- content --> | ||
| + | <p>iGEM stand in METU</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7403-4"><!-- content --> | ||
| + | <p>Keep Calm and Follow Us</p> | ||
| + | </div> | ||
| + | <div class="SSSlideCaption invi clearfix grpelem" id="u7430-4"><!-- content --> | ||
| + | <p>Hülooooğğğğ</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <img class="grpelem" id="u5739-4" src="http://ghucuk.netii.net/images/u5739-4.png" alt="Check Our Gallery" width="218" height="22"/><!-- rasterized frame --> | ||
| + | </div> | ||
| + | <img class="colelem" id="u9876-4" src="http://ghucuk.netii.net/images/u9876-4.png" alt="Thanks to our sponsors" width="247" height="19"/><!-- rasterized frame --> | ||
| + | <div class="colelem" id="u9877"><!-- image --> | ||
| + | <img class="block" id="u9877_img" src="http://ghucuk.netii.net/images/nanobiz.png" alt="" width="96" height="107"/> | ||
| + | </div> | ||
<div class="verticalspacer"></div> | <div class="verticalspacer"></div> | ||
<div class="clearfix colelem" id="u5143-3"><!-- content --> | <div class="clearfix colelem" id="u5143-3"><!-- content --> | ||
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Revision as of 20:42, 2 October 2013
As a well-known fact, bees take the major part in pollination, which leads to give fruit, while preparing honey. During the last few decades, some powerful insecticides like imidacloprid were developed to fight with harmful insects; however, the ability to kill almost all insects started to affect other insects, which they must not have affected from the beginning.
This was the start point of our project. We, as a group formed by mostly biological sciences students, knew that no ecological system can maintain without bees. When we looked into the source of the problem, it was clearly revealed that we couldn’t stop usage of imidacloprid. Then, we came up with an idea to protect bees against existing imidacloprid. Having known that drosophila has some sort of immunity to this insecticide, we planned to use it.
In this year, in the kill switch part of our project, we use an mRNA interferase, MazF, which cleaves mRNA’s at specific sequences. In our kill switch, we used anti-sense RNA principle as a template. According to this principle, MazF, which is constantly produced via a constitutive promoter, is got inactivated by Anti-MazF construct. In order to trigger this mechanism, we used IPTG, a harmless molecule for the bee which at the same time does not appear in the honey too. When IPTG is present in the environment, LacI is inhibited by IPTG and therefore promoter gets activated. With the activated promoter of it, Anti-MazF is produced and inactive MazF. As long as IPTG exists, MazF gets inactivated continuously; therefore, the bacteria maintain their lives. On the other hand, if bacteria exists in a IPTG-free environment, Anti-MazF producing stops, which leads to MazF producing and bacteria get killed by MazF.
In this circuit, we used the parts K143053 as a constitutive promoter and SpoVG RBS due to their more efficient works in Bacillus subtilis. Besides these two, we used the part I732820 to produce LacI, but we had to change the RBS of this system since RBSelowitz is less effective in Bacillus then RBSspoVG. With this change in the activity of the RBS, we can compete with the production of MazF.
Imidacloprid Immunity
Immune System Boost
Kill Switch
.jpg)
58x43.jpg){kind=small}
First meeting of METU iGEM '13 team
It was a snowy day when we fell in love with iGEM.
The aseptic conditions are satisfied.
Researching for our project.
Optimizations for lab work
Collaboration with ITU iGEM team @ Istanbul
Collaboration with ITU iGEM team @ Istanbul
Seminar about synthetic biology given by us to METU College students
Hanging out with Baskent Uni iGEM team
Modelling team is working, not playing LoL.
Participating in Treasure Hunt '13 in METU
Modelling team saying goodbye to our captain, Buse Reis, who kept in touch with us in Cambridge.
Strengthening our bonds by group activities
The collaboration of all iGEM teams in Turkey. We presented our projects to each other and our advisors to improve them and to get feedback.
Making an introduction to lab works for ITU MOBGAM team
Making an introduction to lab works for ITU MOBGAM team
Safety first :D
#direnplate
One of our meetings with Assoc. Prof. Dr. Mesut MUYAN
Collaboration and education with mechanical engineers of METU
Ancient autoclave found in METU Biological Sciences building
Planning the circuits
Hosting future iGEMers from Güven Eğitim & Sağlık Vakfı in METU
Young iGEMers appreciating science
Our captain after doing hard laboratory work :D
SCIENCE B*TCH
Enjoying our bee cookies aseptically in lab
Thanks to Nihal'in Elinden for cookies
Enjoying our bee cookies not so very aseptically in lab
Falcon quality control :p
Modelling team at work
Raising awareness about synthetic biology in METU
Brainstorming for the project
SynBio Party poster
SynBio Party cocktails
SynBio Party people having fun
SynBio Party
SynBio Party 300 -500
SynBio Party
SynBio Party :D
SynBio Party which made us some funds
Synthetic Biology Day Crew
Our beloved participants in Synthetic Biology day
Synthetic Biology Day Crew and Participants
iGEM Stand in METU
iGEM stand in METU
Keep Calm and Follow Us
Hülooooğğğğ
