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Team:DTU-Denmark/Notebook/9 July 2013
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==Procedure== | ==Procedure== | ||
| - | Run gel: | + | ===Run gel:=== |
* 1ul of dye | * 1ul of dye | ||
* 5ul of sample | * 5ul of sample | ||
* 5ul Kb ladder | * 5ul Kb ladder | ||
| - | Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit) | + | ===Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)=== |
| - | + | ||
| - | + | ||
| + | ===PCR=== | ||
==Results== | ==Results== | ||
Revision as of 15:33, 9 July 2013
Contents |
208
Main purpose
Run gel with 7 Nir operon from Pseudomonas aeruginosa and purified AMO, HAO and CYC from 08.07.2013.
Purification of Nir operon and Nanodrop measurement.
Who was in the lab
Ariadni,,Henrike,Julia
Procedure
Run gel:
- 1ul of dye
- 5ul of sample
- 5ul Kb ladder
Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)
PCR
Results
Nanodrop measurement:
- ng/ul (Nir)
- ng/ul (Nir)
- ng/ul (Nir)
