Team:UNITN-Trento/Notebook/Labposts/06/10

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{
{
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"date" : "2013-06-07",
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"date" : "2013-06-10",
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"author" : "viola-fabio-emil",
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"author" : "michele-emil",
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"title" : "Purification and PCR of pSB1C3 linearized, gel",
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"title" : "Transformation of the product of ligation SAMsynthetase + psB1C3.",
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"content" : "<html>We purified with the Quick Reference purification kit(GE) and made a PCR of the linearized plasmid pSB1C3 using the prefix and suffix primers  and the Phusion polimerase following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Phusion-PCR\">its protocol</a>. Then we took the PCR samples and checked the results with an electrophoresis gel  using the ladder Generuler 1 kb Plus DNA Ladder of Fermentas:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><img id=\"post_img\" src=\" https://static.igem.org/mediawiki/2013/8/83/Tn-20130607-pSB1C3_linear.png\" width=\"550px\"/></html>}}<html>As we can see the plasmid, stopped at 2 kb ca (pSB1C3=2070kb).</html>",
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"content" : "<html>We took the ligation products (SAMsynthetase + pSB1C3 in two different rapports 2:1 and 1:!) done by Caterina and Fabio in the morning and we transformed it in NEB10 cells. We've also prepared some inocula from the plates of the devices extract in CiBio (BBa_J45700, BBa_J45320, BBa_J45119)in LB with the correct antibioticum to have a lot of materials to work on.</html>",
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"tags" : "pSB1C3"
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"tags" : "SAMsynthetase-PchB-PchBA-BSMT1"
}
}

Latest revision as of 07:41, 3 October 2013

{ "date" : "2013-06-10", "author" : "michele-emil", "title" : "Transformation of the product of ligation SAMsynthetase + psB1C3.", "content" : "We took the ligation products (SAMsynthetase + pSB1C3 in two different rapports 2:1 and 1:!) done by Caterina and Fabio in the morning and we transformed it in NEB10 cells. We've also prepared some inocula from the plates of the devices extract in CiBio (BBa_J45700, BBa_J45320, BBa_J45119)in LB with the correct antibioticum to have a lot of materials to work on.", "tags" : "SAMsynthetase-PchB-PchBA-BSMT1" }

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