Team:UNITN-Trento/Notebook/Labposts/06/22

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-14",
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"date" : "2013-06-13",
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"author" : "bruno",
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"author" : "caterina-michele",
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"title" : "Transformation efficiency kit results",
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"title" : " A Long Day",
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"content" : "<html>The day after transformation I have check the grow of the our cell line and I have find only 4 colony in the more concentrated plate with DNA. This results is probably due to the very low concentration of DNA insert in the cell line (only 50pg of DNA). However, the presence of cell indicates that the efficiency of the cell is sufficient for our experiments.</html>",
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"content" : " <html>We took 4 inocula of <b>pSB1C3 </b> and we performed the purification (Wizard Plus SV Miniprep DNA purification system). Then we performed a digestion with EcoRI and PstI of this 4 minipreps, SAM (another time because we were not satisfied of the previous results!) and two pSB1C3. After the digestion we did a gel to understand if it was our lucky day. As you can see from the picture, evidently not! </html> {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><img id=\"post_img\" src=\"https://static.igem.org/mediawiki/2013/0/0a/Tn-20130613-Super_Gel_screening_8_cose.jpg\" width=\"500px\"/> </html>}}<html>In the afternoon we prepared two PCR reactions: one for J45700 (from the miniprep = it’s the complete device!) and one to linearize and to amplify the pSB1C3 vector. Both the reactions failed and we were too disappointed to take a picture of the gel.Finally we made another digestion for J45319, J45119 and Cate’s pSB1C3  with EcoRI and PstI. </html>",
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"tags" : "RFP"
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"tags" : "PchBA-PchA-PchB"
}
}

Latest revision as of 07:44, 3 October 2013

{ "date" : "2013-06-13", "author" : "caterina-michele", "title" : " A Long Day", "content" : " We took 4 inocula of pSB1C3 and we performed the purification (Wizard Plus SV Miniprep DNA purification system). Then we performed a digestion with EcoRI and PstI of this 4 minipreps, SAM (another time because we were not satisfied of the previous results!) and two pSB1C3. After the digestion we did a gel to understand if it was our lucky day. As you can see from the picture, evidently not!

Gel Image
In the afternoon we prepared two PCR reactions: one for J45700 (from the miniprep = it’s the complete device!) and one to linearize and to amplify the pSB1C3 vector. Both the reactions failed and we were too disappointed to take a picture of the gel.Finally we made another digestion for J45319, J45119 and Cate’s pSB1C3 with EcoRI and PstI. ", "tags" : "PchBA-PchA-PchB" }

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