Team:UNITN-Trento/Notebook/Labposts/06/45

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{
{
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"date" : "2013-06-20",
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"date" : "2013-06-21",
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"author" : "emil-gabriele",
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"author" : "thomas",
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"title" : "Linearization of psB1C3(Failed) and screening of SAM sinthethase",
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"title" : "Inocula of AraCpBAD + EFE in pSB1C3",
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"content" : "<html>We quantified yesterday inocula (1:1, 1:2, 1:4 of SAMsynthetase and R0010, both in pSB1C3).</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantification (ng/&micro;l)</th></tr><tr><td>1:1 A</td><td>293.0</td></tr><tr><td>1:1 B</td><td>135.7</td></tr><tr><td>1:1 C</td><td>178.0</td></tr><tr><td>1:1 D</td><td>139.3</td></tr><tr><td>1:1 E</td><td>262.0</td></tr><tr><td>1:1 F</td><td>147.5</td></tr><tr><td>1:1 G</td><td>212.5</td></tr><tr><td>1:1 H</td><td>210.2</td></tr><tr><td>1:2 A</td><td>141.0</td></tr><tr><td>1:2 B</td><td>123.6</td></tr><tr><td>1:4 A</td><td>207.8</td></tr><tr><td>1:4 B</td><td>127.2</td></tr><tr><td>R0010 A</td><td>131.7</td></tr><tr><td>R0010 B</td><td>119.6</td></tr><tr><td>R0010 C</td><td>371.0</td></tr><tr><td>R0010 D</td><td>307.9</td></tr><tr><td>R0010 E</td><td>313.8</td></tr></table></center></html>}}<html>Then, we screened the products of ligation (1:1, 1:2, 1:4 and R0010): digestion with EcoRI-HF and PstI-HF following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digestion protocol</a> and agarose gel 1%.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading Scheme</th></tr><tr><td><i>Empty</i></td></tr><tr><td>1kb ladder</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1:1 D</td></tr><tr><td>1:1 E</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1:2 B</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1:4 A</td></tr><tr><td><i>Empty</i></td></tr><tr><td>R0010 E</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/5/54/Tn-20130620-ET_GG_screening_SAM_R0010_Failed.jpg\" width=\"450px\" /> <!-- Sams primo screening--></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td>1kb ladder</td></tr><tr><td>R0010 A</td></tr><tr><td>R0010 B</td></tr><tr><td>R0010 C</td></tr><tr><td>R0010 D</td></tr><tr><td>R0010 E</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/1/1d/Tn-20130620-ET_screening_psB1C3SAM_FAILED.jpg\" width=\"450px\" /> <!-- Sams 1:1 and PCR(last 3) --></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td>R0010 B</td></tr><tr><td>R0010 C</td></tr><tr><td>R0010 D</td></tr><tr><td>1kb ladder</td></tr><tr><td>1:4 A</td></tr><tr><td>1:4 B</td></tr><tr><td>1:2 A</td></tr><tr><td>1:2 B</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/a/aa/Tn-20130620-GG_R0010_pSB1C3SAM_FAILED.jpg\" width=\"450px\" /> <!-- R0010 --></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1.5% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td>1kb ladder</td></tr><tr><td>R0010 A</td></tr><tr><td>R0010 B</td></tr><tr><td>R0010 C</td></tr><tr><td>R0010 D</td></tr><tr><td>R0010 E</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/8/8b/Tn-20130620-GG_R0010_screening_FAILED.jpg\" width=\"450px\" /> <!-- R0010 bis --></center></html>}}<html>In these images we can only see the band of the backbone and no insert!</html>",
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"content" : "I inoculated the previously transformed AraCpBAD + EFE in pSB1C3 into 5ml of LB containing Chloramphenicol.{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Inocula image|<html><center><img id=\"post_img\" src=\"https://static.igem.org/mediawiki/2013/2/2e/Tn-20130621-Inocula_TP.JPG\" width=\"400px\"/></center></html>}}As you can see, it seems that all the inocula grown. I will proceed with the purification and the screening test!",
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"tags" : "SAMsynthetase"
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"tags" : "EFE-AraCpBAD"
}
}

Latest revision as of 07:52, 3 October 2013

{ "date" : "2013-06-21", "author" : "thomas", "title" : "Inocula of AraCpBAD + EFE in pSB1C3", "content" : "I inoculated the previously transformed AraCpBAD + EFE in pSB1C3 into 5ml of LB containing Chloramphenicol.

Inocula image
As you can see, it seems that all the inocula grown. I will proceed with the purification and the screening test!", "tags" : "EFE-AraCpBAD" }

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