From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-06-26", | + | "date" : "2013-06-24", |
| | "author" : "emil-gabriele", | | "author" : "emil-gabriele", |
| - | "title" : "Ligation and transformation of R0010 + Sam sinthethase", | + | "title" : "Inocula of R0010", |
| - | "content" : "<html>Today we added 1µl of SAP to the o/n digestion and then incubated it for 1.5h.Then we performed the ligation of SAMynthetase (previously digested with XbaI and PstI), following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\">ligation protocol</a>.<br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Ligation mixes|<html><center><table class=\"tn-sp-table\"><tr><td style=\"border:none\"></td><th>Ctrl</th><th>1:1</th><th>1:3</th></tr><tr><td>Buffer</td><td colspan=\"3\">2.5µl</td></tr><tr><td>plasmid</td><td colspan=\"3\">5.73µl</td></tr><tr><td>insert</td><td>0</td><td>3.09µl</td><td>9.27µl</td></tr><tr><td>ligase</td><td colspan=\"3\">1µl</td></tr><tr><td>water</td><td>15.77µl</td><td>12.68µl</td><td>6.50µl</td></tr></table></center></html>}}<html>Afterwards, Gabriele purified the product following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel\">usual purification protocol</a> and then transformed it in NEB10β cells following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation protocol</a>.<br/><br/>Gabriele prepared also 4 inocula from the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Notebook#tn-post-2013-06-18-gabriele-emil\">pSB1C3+R0010 CM-plate</a>, just to be sure that it is (not) there.</html>", | + | "content" : "<html>We performed 5 inocula from the plate containing the colonies resulted from the transformation of R0010 (in pSB1A2).<br/>The LB medium contained Ampicillin (1:1000).</html>", |
| - | "tags" : "Plac-SAMsynthetase" | + | "tags" : "Plac" |
| | } | | } |
Latest revision as of 07:55, 3 October 2013
{
"date" : "2013-06-24",
"author" : "emil-gabriele",
"title" : "Inocula of R0010",
"content" : "We performed 5 inocula from the plate containing the colonies resulted from the transformation of R0010 (in pSB1A2).
The LB medium contained Ampicillin (1:1000).",
"tags" : "Plac"
}
"
