Team:UNITN-Trento/Notebook/Labposts/06/58

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-10",
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"date" : "2013-06-26",
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"author" : "fabio-caterina",
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"author" : "viola",
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"title" : "Purification of SAMsynthetase and pSB1C3.",
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"title" : "Adding the terminator to EFE: day 3",
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"content" : "<html>We purified the products of SAMsynthetase and pSB1C3 PCR performed by Gire and Pedro following the illustra™ GFX™ PCR DNA and Gel Band Purification Kit protocol. After the quantification with NanoDrop, we did the ligation reaction.</html>",
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"content" : "<html>i miniprepped the inocula from the transformation of B0015 both in psb1c3 and psb1ak3 and i made a 2% gel for screening the little terminator. I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/37/Tn-2013_Corsa20minPCR_B0015.jpg\" width=\"450px\" /></center></html>}}<html> on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.</html>",
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"tags" : "SAMsynthetase"
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"tags" : "EFE-B0015"
}
}

Latest revision as of 07:57, 3 October 2013

{ "date" : "2013-06-26", "author" : "viola", "title" : "Adding the terminator to EFE: day 3", "content" : "i miniprepped the inocula from the transformation of B0015 both in psb1c3 and psb1ak3 and i made a 2% gel for screening the little terminator. I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :

Gel image
on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.", "tags" : "EFE-B0015" }

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