Team:UNITN-Trento/Notebook/Labposts/06/68
From 2013.igem.org
(Difference between revisions)
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{ | { | ||
- | "date" : "2013-06- | + | "date" : "2013-06-28", |
- | "author" : "thomas | + | "author" : "thomas", |
- | "title" : " | + | "title" : "Ethylene detection through microGC!!!", |
- | "content" : " | + | "content" : "<html>In order to see if our EFE enzyme is effectively active and functional I made a gas-cromatography analysis.<br/> Starting from an overnight culture, I diluted it 1:100 and I incubated it until it reached 0,5 O.D.600. After that, I added 5mM of Arabinose and I incubated the culture at 37°C for about 4-5 hours. In the I connected the vial previously keeped ermetically closed at the micro GC and I took the measure. I did this work for three samples: negative control (not induced), 5mM Arabinose V=1,5ml and 5mM Arabinose V=3ml. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Results table|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Ethylene detected</th></tr><tr><td>Not induced</td><td>0±15 ppm </td></tr><tr><td>5mM Arabinose V=1,5ml</td><td>30±15 ppm </td></tr><tr><td>5mM Arabinose V=3ml</td><td>70±15 ppm </td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Apparatus image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/8/8c/Tn-20130628-Apparatus.JPG\" style =\"width: 450px\"></center></html>}}<html>As you can see, seems that the device is correct and functionally active! Definetely a good result!</html>", |
- | "tags" : " | + | "tags" : "EFE" |
} | } |
Latest revision as of 08:00, 3 October 2013
{
"date" : "2013-06-28",
"author" : "thomas",
"title" : "Ethylene detection through microGC!!!",
"content" : "In order to see if our EFE enzyme is effectively active and functional I made a gas-cromatography analysis.
Starting from an overnight culture, I diluted it 1:100 and I incubated it until it reached 0,5 O.D.600. After that, I added 5mM of Arabinose and I incubated the culture at 37°C for about 4-5 hours. In the I connected the vial previously keeped ermetically closed at the micro GC and I took the measure. I did this work for three samples: negative control (not induced), 5mM Arabinose V=1,5ml and 5mM Arabinose V=3ml.
Results table
As you can see, seems that the device is correct and functionally active! Definetely a good result!",
"tags" : "EFE"
}
Sample | Ethylene detected |
---|---|
Not induced | 0±15 ppm |
5mM Arabinose V=1,5ml | 30±15 ppm |
5mM Arabinose V=3ml | 70±15 ppm |