Team:UNITN-Trento/Notebook/Labposts/08/50

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(Difference between revisions)

Latest revision as of 09:16, 3 October 2013

{ "date" : "2013-08-19", "author" : "bruno", "title" : "Target acquired, update in progress, that the mutagenesis begin", "content" : "I used the specific primer degnigned and created to insert RBS in the K952003 part before the amilGFP protein. For doing that I phosphorylated the primer with PNK chinase. In addition I doing 2 PCR to mutagenize the sequence using 100pg and 1ng of DNA template. The condition how the PCR works are: 67 degree of anealing, 1.30 mins of extention time and 1.5 ul of DMSO. It took the life the part K1065302", "tags" : "Blue light-mutagenesis" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-25",
+	"date" : "2013-08-19",
-	"author" : "caterina",
+	"author" : "bruno",
-	"title" : "<html>The last clonation gives me no satisfaction</html>",
+	"title" : "<html>Target acquired, update in progress, that the mutagenesis begin</html>",
-	"content" : "<html>In these days I tried to clone EFE in the blue light device but unfortunately I failed more and more times. </html>",
+	"content" : "<html>I used the specific primer degnigned and created to insert RBS in the K952003 part before the amilGFP protein. For doing that I phosphorylated the primer with PNK chinase. In addition I doing 2 PCR to mutagenize the sequence using 100pg and 1ng of DNA template. The condition how the PCR works are: 67 degree of anealing, 1.30 mins of extention time and 1.5 ul of DMSO. It took the life the part K1065302</html>",
-	"tags" : "blue_light-ethylene"
+	"tags" : "Blue light-mutagenesis"
 }