Team:UNITN-Trento/Notebook/Labposts/08/56

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(Difference between revisions)

Latest revision as of 09:18, 3 October 2013

{ "date":"2013-08-31", "author":"fabio", "title":" the blue circuit doesn’t always work!! ", "content":" I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination. ", "tags":"blue_light-EFE" }

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 {
-	"date" : "2013-08-03",
+	"date":"2013-08-31",
-	"author" : "gabriele",
+	"author":"fabio",
-	"title" : "Wintergreen with pLac-SAMsynthetase: Ep. II",
+	"title":" <html> the blue circuit doesn’t always work!!</html> ",
-	"content" : "<html>I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/&micro;l) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!! </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>13A</td></tr><tr><td>13B</td></tr><tr><td>13C</td></tr><tr><td><i>Empty</i></td></tr><tr><td>14A</td></tr><tr><td>14B</td></tr><tr><td>14C</td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/f/f7/Tn-20130803_SAMinWGREEN_check_2.jpg\" /></html>}}<html>Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>1kb ladder</td></tr><tr><td>13A</td></tr><tr><td>Ctrl</td></tr><tr><td><i>14B</i></td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/7/76/Tn-20130803_SAMinWGREEN_check_3.jpg\" /></html>}}<html></html>",
+	"content":" <html> I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination.</html> ",
-	"tags" : "Wintergreen-SAMsynthetase-pLac"
+	"tags":"blue_light-EFE"
 }